##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AspI BamHI
BbeI BcgI BsaHI BsiEI BsiWI
BspHI BspLU11I BssHII Bst1107I BstZ17I
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoT22I FseI Hin1I HincII
HindII HpaI KasI KpnI KspI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 2419 GTAGAC GT^[AC][GT]AC
AccII 156 CGCG CG^CG
AccIII 162 TCCGGA T^CCGGA
AflII 829 CTTAAG C^TTAAG
Alw44I 3267 GTGCAC G^TGCAC
Aor51HI 2877 AGCGCT AGC^GCT
ApaLI 3267 GTGCAC G^TGCAC
AvaI 184 CCCGAG C^[CT]CG[GA]G
AviII 624 TGCGCA TGC^GCA
BciVI 2969 GGATAC GTATCC(6/5)
BfrI 829 CTTAAG C^TTAAG
BglI 283 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 63 AGATCT A^GATCT
BlpI 1211 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1211 GCTAAGC GC^T[ACGT]AGC
BsaBI 64 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 162 TCCGGA [AT]^CCGG[AT]
BseAI 162 TCCGGA T^CCGGA
BsgI 3729 CTGCAC GTGCAG(16/14)
BsoBI 184 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3821 TGTACA T^GTACA
BspDI 598 ATCGAT AT^CGAT
BspEI 162 TCCGGA T^CCGGA
BsrBI 482 GAGCGG CCGCTC(-3/-3)
BsrGI 3821 TGTACA T^GTACA
BssSI 1224 CTCGTG CACGAG(-5/-1)
BstAPI 2849 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 887 TTCGAA TT^CGAA
BstEII 732 GGTCACC G^GT[ACGT]ACC
BstPI 732 GGTCACC G^GT[ACGT]ACC
BstUI 156 CGCG CG^CG
CelII 1211 GCTAAGC GC^T[ACGT]AGC
ClaI 598 ATCGAT AT^CGAT
DraI 3151 TTTAAA TTT^AAA
DraIII 1142 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2877 AGCGCT AGC^GCT
EcoO65I 732 GGTCACC G^GT[ACGT]ACC
EcoRI 1063 GAATTC G^AATTC
EcoRV 1628 GATATC GAT^ATC
FspI 624 TGCGCA TGC^GCA
HaeII 2877 AGCGCT [GA]GCGC^[CT]
MamI 64 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 162 TCCGGA T^CCGGA
MvnI 156 CGCG CG^CG
NcoI 2029 CCATGG C^CATGG
NspV 887 TTCGAA TT^CGAA
PflMI 1141 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI 887 TTCGAA TT^CGAA
SmlI 829 CTTAAG C^T[CT][GA]AG
SpeI 1920 ACTAGT A^CTAGT
SphI 1278 GCATGC GCATG^C
SspBI 3821 TGTACA T^GTACA
StuI 2255 AGGCCT AGG^CCT
Van91I 1141 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 1828 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3840 GAAGCATTTC
AvrII 1991 CCTAGG C^CTAGG
2218 CCTAGG
BanI 965 GGCACC G^G[CT][GA]CC
2153 GGCACC
BbrPI 1467 CACGTG CAC^GTG
2067 CACGTG
BcnI 403 CCGGG CC^[GC]GG
969 CCGGG
BlnI 1991 CCTAGG C^CTAGG
2218 CCTAGG
BsaAI 1467 CACGTG [CT]AC^GT[GA]
2067 CACGTG
BsmI 2601 GAATGC GAATGC(1/-1)
2912 GCATTC
BspMI 130 GCAGGT ACCTGC(4/8)
1790 GCAGGT
BsrDI 487 GCAATG GCAATG(2/0)
3832 GCAATG
BsrFI 139 GCCGGT [GA]^CCGG[CT]
460 ACCGGC
Cfr10I 139 GCCGGT [GA]^CCGG[CT]
460 ACCGGC
DsaI 1146 CCGTGG C^C[GA][CT]GG
2029 CCATGG
EcoNI 2144 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2157 CCTTTGATAGG
FauI 114 GCGGG CCCGC(4/6)
266 GCGGG
MfeI 573 CAATTG C^AATTG
1911 CAATTG
MunI 573 CAATTG C^AATTG
1911 CAATTG
NciI 403 CCGGG CC^[GC]GG
969 CCGGG
PmaCI 1467 CACGTG CAC^GTG
2067 CACGTG
PmlI 1467 CACGTG CAC^GTG
2067 CACGTG
PstI 128 CTGCAG CTGCA^G
2899 CTGCAG
PvuII 649 CAGCTG CAG^CTG
757 CAGCTG
SacI 187 GAGCTC GAGCT^C
560 GAGCTC
SfaNI 244 GATGC GCATC(5/9)
399 GATGC
XmnI 1828 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3840 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
SapI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII