################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AflII Aor51HI ApaI AscI Asp700I AspI AvrII BamHI BclI BfrI BglI BlnI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FbaI FseI HindIII HpaI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PmeI PshAI PvuI RsrII SalI SapI ScaI SexAI SfiI SfuI SnaBI SpeI SphI SspBI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 3051 GACGTC GACGT^C Acc65I 1535 GGTACC G^GTACC AccI 454 GTCTAC GT^[AC][GT]AC AclI 2946 AACGTT AA^CGTT AhdI 2172 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1842 GTGCAC G^TGCAC ApaLI 1842 GTGCAC G^TGCAC AseI 2598 ATTAAT AT^TAAT AsnI 2598 ATTAAT AT^TAAT Asp718I 1535 GGTACC G^GTACC AspEI 2172 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 1427 GGCGCC GGCGC^C BbvCI 29 GCTGAGG CCTCAGC(-5/-2) BcgI 3272 GCACTGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 480 GTATCC GTATCC(6/5) BlpI 1061 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1061 GCTGAGC GC^T[ACGT]AGC BsaBI 2784 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1025 GAGACG CGTCTC(1/5) BspHI 1711 TCATGA T^CATGA BspLU11I 1924 ACATGT A^CATGT BssSI 1989 CACGAG CACGAG(-5/-1) CelII 1061 GCTGAGC GC^T[ACGT]AGC DraIII 2579 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2172 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 3305 GATATC GAT^ATC EcoT22I 3041 ATGCAT ATGCA^T HaeII 1427 GGCGCC [GA]GCGC^[CT] HincII 819 GTCAAC GT[CT]^[GA]AC HindII 819 GTCAAC GT[CT]^[GA]AC KasI 1427 GGCGCC G^GCGCC KpnI 1535 GGTACC GGTAC^C KspI 1882 CCGCGG CCGC^GG MamI 2784 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1427 GGCGCC GG^CGCC NsiI 3041 ATGCAT ATGCA^T PaeR7I 1951 CTCGAG C^TCGAG PshBI 2598 ATTAAT AT^TAAT Psp1406I 2946 AACGTT AA^CGTT RcaI 1711 TCATGA T^CATGA SacII 1882 CCGCGG CCGC^GG SbfI 119 CCTGCAGG CCTGCA^GG SfoI 1427 GGCGCC GGC^GCC SgrAI 2182 CGCCGGTG C[GA]^CCGG[CT]G SmaI 2354 CCCGGG CCC^GGG Sse8387I 119 CCTGCAGG CCTGCA^GG XcmI 3375 CCACCCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1951 CTCGAG C^TCGAG XmaCI 2354 CCCGGG C^CCGGG XmaI 2354 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1437 AAATTC [GA]^AATT[CT] 2280 AAATTC AlwNI 577 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1089 CAGAGCCTG AocI 639 CCTAAGG CC^T[ACGT]AGG 1250 CCTGAGG ApoI 1437 AAATTC [GA]^AATT[CT] 2280 AAATTC AviII 919 TGCGCA TGC^GCA 3210 TGCGCA BalI 595 TGGCCA TGG^CCA 1823 TGGCCA BbrPI 1839 CACGTG CAC^GTG 1890 CACGTG BglII 796 AGATCT A^GATCT 1322 AGATCT BsaAI 1839 CACGTG [CT]AC^GT[GA] 1890 CACGTG BsmI 3043 GCATTC GAATGC(1/-1) 3220 GCATTC Bsu36I 639 CCTAAGG CC^T[ACGT]AGG 1250 CCTGAGG EaeI 595 TGGCCA [CT]^GGCC[GA] 1823 TGGCCA Eco81I 639 CCTAAGG CC^T[ACGT]AGG 1250 CCTGAGG FspI 919 TGCGCA TGC^GCA 3210 TGCGCA MluNI 595 TGGCCA TGG^CCA 1823 TGGCCA MscI 595 TGGCCA TGG^CCA 1823 TGGCCA PflMI 1278 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3379 CCATCCAATGG PmaCI 1839 CACGTG CAC^GTG 1890 CACGTG PmlI 1839 CACGTG CAC^GTG 1890 CACGTG SspI 3181 AATATT AAT^ATT 3325 AATATT StuI 1694 AGGCCT AGG^CCT 3315 AGGCCT Tsp45I 2001 GTCAC ^GT[GC]AC 2798 GTGAC Van91I 1278 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3379 CCATCCAATGG XbaI 950 TCTAGA T^CTAGA 3097 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AgeI AluI AlwI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PinAI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII