##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AflII Aor51HI ApaI AscI
Asp700I AspI AvrII BamHI BclI
BfrI BglI BlnI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FbaI
FseI HindIII HpaI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PmeI PshAI PvuI
RsrII SalI SapI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
SspBI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3051 GACGTC GACGT^C
Acc65I 1535 GGTACC G^GTACC
AccI 454 GTCTAC GT^[AC][GT]AC
AclI 2946 AACGTT AA^CGTT
AhdI 2172 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1842 GTGCAC G^TGCAC
ApaLI 1842 GTGCAC G^TGCAC
AseI 2598 ATTAAT AT^TAAT
AsnI 2598 ATTAAT AT^TAAT
Asp718I 1535 GGTACC G^GTACC
AspEI 2172 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1427 GGCGCC GGCGC^C
BbvCI 29 GCTGAGG CCTCAGC(-5/-2)
BcgI 3272 GCACTGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 480 GTATCC GTATCC(6/5)
BlpI 1061 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1061 GCTGAGC GC^T[ACGT]AGC
BsaBI 2784 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1025 GAGACG CGTCTC(1/5)
BspHI 1711 TCATGA T^CATGA
BspLU11I 1924 ACATGT A^CATGT
BssSI 1989 CACGAG CACGAG(-5/-1)
CelII 1061 GCTGAGC GC^T[ACGT]AGC
DraIII 2579 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2172 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3305 GATATC GAT^ATC
EcoT22I 3041 ATGCAT ATGCA^T
HaeII 1427 GGCGCC [GA]GCGC^[CT]
HincII 819 GTCAAC GT[CT]^[GA]AC
HindII 819 GTCAAC GT[CT]^[GA]AC
KasI 1427 GGCGCC G^GCGCC
KpnI 1535 GGTACC GGTAC^C
KspI 1882 CCGCGG CCGC^GG
MamI 2784 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1427 GGCGCC GG^CGCC
NsiI 3041 ATGCAT ATGCA^T
PaeR7I 1951 CTCGAG C^TCGAG
PshBI 2598 ATTAAT AT^TAAT
Psp1406I 2946 AACGTT AA^CGTT
RcaI 1711 TCATGA T^CATGA
SacII 1882 CCGCGG CCGC^GG
SbfI 119 CCTGCAGG CCTGCA^GG
SfoI 1427 GGCGCC GGC^GCC
SgrAI 2182 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2354 CCCGGG CCC^GGG
Sse8387I 119 CCTGCAGG CCTGCA^GG
XcmI 3375 CCACCCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1951 CTCGAG C^TCGAG
XmaCI 2354 CCCGGG C^CCGGG
XmaI 2354 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1437 AAATTC [GA]^AATT[CT]
2280 AAATTC
AlwNI 577 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1089 CAGAGCCTG
AocI 639 CCTAAGG CC^T[ACGT]AGG
1250 CCTGAGG
ApoI 1437 AAATTC [GA]^AATT[CT]
2280 AAATTC
AviII 919 TGCGCA TGC^GCA
3210 TGCGCA
BalI 595 TGGCCA TGG^CCA
1823 TGGCCA
BbrPI 1839 CACGTG CAC^GTG
1890 CACGTG
BglII 796 AGATCT A^GATCT
1322 AGATCT
BsaAI 1839 CACGTG [CT]AC^GT[GA]
1890 CACGTG
BsmI 3043 GCATTC GAATGC(1/-1)
3220 GCATTC
Bsu36I 639 CCTAAGG CC^T[ACGT]AGG
1250 CCTGAGG
EaeI 595 TGGCCA [CT]^GGCC[GA]
1823 TGGCCA
Eco81I 639 CCTAAGG CC^T[ACGT]AGG
1250 CCTGAGG
FspI 919 TGCGCA TGC^GCA
3210 TGCGCA
MluNI 595 TGGCCA TGG^CCA
1823 TGGCCA
MscI 595 TGGCCA TGG^CCA
1823 TGGCCA
PflMI 1278 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3379 CCATCCAATGG
PmaCI 1839 CACGTG CAC^GTG
1890 CACGTG
PmlI 1839 CACGTG CAC^GTG
1890 CACGTG
SspI 3181 AATATT AAT^ATT
3325 AATATT
StuI 1694 AGGCCT AGG^CCT
3315 AGGCCT
Tsp45I 2001 GTCAC ^GT[GC]AC
2798 GTGAC
Van91I 1278 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3379 CCATCCAATGG
XbaI 950 TCTAGA T^CTAGA
3097 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AgeI AluI AlwI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PinAI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII