################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AcyI AflIII AgeI AhdI Aor51HI AscI AspEI AviII BamHI BbeI BbrPI BciVI BglI BglII BsaAI BsaHI BsaI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BssHII BssSI BstAPI BstBI ClaI DrdI Eam1105I Eco47III EcoRV EcoT22I FseI FspI Hin1I KasI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 4332 GGTACC G^GTACC Alw44I 3751 GTGCAC G^TGCAC AocI 3243 CCTTAGG CC^T[ACGT]AGG ApaI 61 GGGCCC GGGCC^C ApaLI 3751 GTGCAC G^TGCAC AseI 1617 ATTAAT AT^TAAT AsnI 1617 ATTAAT AT^TAAT Asp718I 4332 GGTACC G^GTACC AspI 1146 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 717 CTCGGG C^[CT]CG[GA]G AvrII 3255 CCTAGG C^CTAGG BbvCI 2346 CCTCAGC CCTCAGC(-5/-2) BlnI 3255 CCTAGG C^CTAGG BlpI 4272 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4272 GCTGAGC GC^T[ACGT]AGC BsaBI 2229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 744 GAGACG CGTCTC(1/5) BsoBI 717 CTCGGG C^[CT]CG[GA]G BspMI 2287 ACCTGC ACCTGC(4/8) BsrFI 460 GCCGGT [GA]^CCGG[CT] Bst1107I 4032 GTATAC GTA^TAC BstZ17I 4032 GTATAC GTA^TAC Bsu36I 3243 CCTTAGG CC^T[ACGT]AGG CelII 4272 GCTGAGC GC^T[ACGT]AGC Cfr10I 460 GCCGGT [GA]^CCGG[CT] CpoI 1 CGGTCCG CG^G[AT]CCG DraIII 1492 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 3243 CCTTAGG CC^T[ACGT]AGG EcoNI 1940 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2594 GAATTC G^AATTC HindIII 1135 AAGCTT A^AGCTT HpaI 1092 GTTAAC GTT^AAC KpnI 4332 GGTACC GGTAC^C MamI 2229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1747 CCATGG C^CATGG PacI 1825 TTAATTAA TTAAT^TAA PflFI 1146 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1617 ATTAAT AT^TAAT PstI 3234 CTGCAG CTGCA^G RsrII 1 CGGTCCG CG^G[AT]CCG ScaI 138 AGTACT AGT^ACT SexAI 1832 ACCTGGT A^CC[AT]GGT SpeI 3039 ACTAGT A^CTAGT SspI 3457 AATATT AAT^ATT Tth111I 1146 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4018 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 2594 GAATTC [GA]^AATT[CT] 3682 GAATTT ApoI 2594 GAATTC [GA]^AATT[CT] 3682 GAATTT Asp700I 862 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1180 GAAGCCTTTC BcgI 2389 CGATCACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2451 GCAAGTATGTCG BclI 292 TGATCA T^GATCA 4262 TGATCA BsgI 167 GTGCAG GTGCAG(16/14) 2397 GTGCAG Bsp1407I 562 TGTACA T^GTACA 3721 TGTACA BsrDI 1502 CATTGC GCAATG(2/0) 3592 CATTGC BsrGI 562 TGTACA T^GTACA 3721 TGTACA BstEII 463 GGTCACC G^GT[ACGT]ACC 1836 GGTGACC BstPI 463 GGTCACC G^GT[ACGT]ACC 1836 GGTGACC EagI 27 CGGCCG C^GGCCG 178 CGGCCG EclXI 27 CGGCCG C^GGCCG 178 CGGCCG Eco52I 27 CGGCCG C^GGCCG 178 CGGCCG EcoO65I 463 GGTCACC G^GT[ACGT]ACC 1836 GGTGACC FbaI 292 TGATCA T^GATCA 4262 TGATCA NheI 3485 GCTAGC G^CTAGC 3489 GCTAGC NspI 2742 GCATGT [GA]CATG^[CT] 3169 ACATGC PflMI 256 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1575 CCAGTCCCTGG SspBI 562 TGTACA T^GTACA 3721 TGTACA Van91I 256 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1575 CCAGTCCCTGG XmnI 862 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1180 GAAGCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AccII AciI AfaI AflII AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV PleI PpuMI PvuII RcaI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII