##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI Aor51HI AscI AspEI
AviII BamHI BbeI BbrPI BciVI
BglI BglII BsaAI BsaHI BsaI
BsaWI BseAI BsiWI BspDI BspEI
BspLU11I BssHII BssSI BstAPI BstBI
ClaI DrdI Eam1105I Eco47III EcoRV
EcoT22I FseI FspI Hin1I KasI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4332 GGTACC G^GTACC
Alw44I 3751 GTGCAC G^TGCAC
AocI 3243 CCTTAGG CC^T[ACGT]AGG
ApaI 61 GGGCCC GGGCC^C
ApaLI 3751 GTGCAC G^TGCAC
AseI 1617 ATTAAT AT^TAAT
AsnI 1617 ATTAAT AT^TAAT
Asp718I 4332 GGTACC G^GTACC
AspI 1146 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 717 CTCGGG C^[CT]CG[GA]G
AvrII 3255 CCTAGG C^CTAGG
BbvCI 2346 CCTCAGC CCTCAGC(-5/-2)
BlnI 3255 CCTAGG C^CTAGG
BlpI 4272 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4272 GCTGAGC GC^T[ACGT]AGC
BsaBI 2229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 744 GAGACG CGTCTC(1/5)
BsoBI 717 CTCGGG C^[CT]CG[GA]G
BspMI 2287 ACCTGC ACCTGC(4/8)
BsrFI 460 GCCGGT [GA]^CCGG[CT]
Bst1107I 4032 GTATAC GTA^TAC
BstZ17I 4032 GTATAC GTA^TAC
Bsu36I 3243 CCTTAGG CC^T[ACGT]AGG
CelII 4272 GCTGAGC GC^T[ACGT]AGC
Cfr10I 460 GCCGGT [GA]^CCGG[CT]
CpoI 1 CGGTCCG CG^G[AT]CCG
DraIII 1492 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3243 CCTTAGG CC^T[ACGT]AGG
EcoNI 1940 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2594 GAATTC G^AATTC
HindIII 1135 AAGCTT A^AGCTT
HpaI 1092 GTTAAC GTT^AAC
KpnI 4332 GGTACC GGTAC^C
MamI 2229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1747 CCATGG C^CATGG
PacI 1825 TTAATTAA TTAAT^TAA
PflFI 1146 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1617 ATTAAT AT^TAAT
PstI 3234 CTGCAG CTGCA^G
RsrII 1 CGGTCCG CG^G[AT]CCG
ScaI 138 AGTACT AGT^ACT
SexAI 1832 ACCTGGT A^CC[AT]GGT
SpeI 3039 ACTAGT A^CTAGT
SspI 3457 AATATT AAT^ATT
Tth111I 1146 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4018 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 2594 GAATTC [GA]^AATT[CT]
3682 GAATTT
ApoI 2594 GAATTC [GA]^AATT[CT]
3682 GAATTT
Asp700I 862 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1180 GAAGCCTTTC
BcgI 2389 CGATCACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2451 GCAAGTATGTCG
BclI 292 TGATCA T^GATCA
4262 TGATCA
BsgI 167 GTGCAG GTGCAG(16/14)
2397 GTGCAG
Bsp1407I 562 TGTACA T^GTACA
3721 TGTACA
BsrDI 1502 CATTGC GCAATG(2/0)
3592 CATTGC
BsrGI 562 TGTACA T^GTACA
3721 TGTACA
BstEII 463 GGTCACC G^GT[ACGT]ACC
1836 GGTGACC
BstPI 463 GGTCACC G^GT[ACGT]ACC
1836 GGTGACC
EagI 27 CGGCCG C^GGCCG
178 CGGCCG
EclXI 27 CGGCCG C^GGCCG
178 CGGCCG
Eco52I 27 CGGCCG C^GGCCG
178 CGGCCG
EcoO65I 463 GGTCACC G^GT[ACGT]ACC
1836 GGTGACC
FbaI 292 TGATCA T^GATCA
4262 TGATCA
NheI 3485 GCTAGC G^CTAGC
3489 GCTAGC
NspI 2742 GCATGT [GA]CATG^[CT]
3169 ACATGC
PflMI 256 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCAGTCCCTGG
SspBI 562 TGTACA T^GTACA
3721 TGTACA
Van91I 256 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCAGTCCCTGG
XmnI 862 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1180 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AfaI AflII
AluI AlwI AlwNI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV PleI PpuMI PvuII RcaI
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII