##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI BamHI BbeI BbrPI
BbvCI BcgI BfrI BsaAI BsaHI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII BssSI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI HaeII Hin1I HindIII
HpaI KasI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SfiI SfoI SgrAI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
Acc65I 919 GGTACC G^GTACC
AccI 3651 GTATAC GT^[AC][GT]AC
AccII 5 CGCG CG^CG
AccIII 1312 TCCGGA T^CCGGA
Asp718I 919 GGTACC G^GTACC
AspI 3494 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3939 TGCGCA TGC^GCA
AvrII 248 CCTAGG C^CTAGG
BalI 526 TGGCCA TGG^CCA
BciVI 596 GTATCC GTATCC(6/5)
BclI 3827 TGATCA T^GATCA
BglI 848 GCCGAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3425 AGATCT A^GATCT
BlnI 248 CCTAGG C^CTAGG
BlpI 1188 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1188 GCTGAGC GC^T[ACGT]AGC
BsaBI 772 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1312 TCCGGA [AT]^CCGG[AT]
BseAI 1312 TCCGGA T^CCGGA
BsmI 1587 GCATTC GAATGC(1/-1)
BspEI 1312 TCCGGA T^CCGGA
BspMI 585 ACCTGC ACCTGC(4/8)
BsrDI 1215 GCAATG GCAATG(2/0)
BsrFI 681 ACCGGC [GA]^CCGG[CT]
Bst1107I 3651 GTATAC GTA^TAC
BstAPI 423 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 671 TTCGAA TT^CGAA
BstEII 3446 GGTCACC G^GT[ACGT]ACC
BstPI 3446 GGTCACC G^GT[ACGT]ACC
BstUI 5 CGCG CG^CG
BstYI 3425 AGATCT [GA]^GATC[CT]
BstZ17I 3651 GTATAC GTA^TAC
CelII 1188 GCTGAGC GC^T[ACGT]AGC
CfoI 3940 GCGC GCG^C
Cfr10I 681 ACCGGC [GA]^CCGG[CT]
DraI 1569 TTTAAA TTT^AAA
DraIII 796 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 248 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3446 GGTCACC G^GT[ACGT]ACC
EcoRI 3766 GAATTC G^AATTC
FbaI 3827 TGATCA T^GATCA
FspI 3939 TGCGCA TGC^GCA
HgaI 872 GCGTC GACGC(5/10)
HhaI 3940 GCGC GCG^C
HinP1I 3940 GCGC G^CGC
KpnI 919 GGTACC GGTAC^C
KspI 4 CCGCGG CCGC^GG
MamI 772 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3425 AGATCT [GA]^GATC[CT]
MluNI 526 TGGCCA TGG^CCA
MroI 1312 TCCGGA T^CCGGA
MscI 526 TGGCCA TGG^CCA
MvnI 5 CGCG CG^CG
NcoI 1375 CCATGG C^CATGG
NspV 671 TTCGAA TT^CGAA
PflFI 3494 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 4 CCGCGG CCGC^GG
SapI 1358 GAAGAGC GCTCTTC(1/4)
SbfI 586 CCTGCAGG CCTGCA^GG
ScaI 1467 AGTACT AGT^ACT
SexAI 3442 ACCAGGT A^CC[AT]GGT
SfuI 671 TTCGAA TT^CGAA
SmaI 105 CCCGGG CCC^GGG
SpeI 3958 ACTAGT A^CTAGT
SphI 1646 GCATGC GCATG^C
Sse8387I 586 CCTGCAGG CCTGCA^GG
Tth111I 3494 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 3425 AGATCT [GA]^GATC[CT]
XmaCI 105 CCCGGG C^CCGGG
XmaI 105 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1851 GAATTT [GA]^AATT[CT]
3766 GAATTC
ApoI 1851 GAATTT [GA]^AATT[CT]
3766 GAATTC
AvaI 105 CCCGGG C^[CT]CG[GA]G
210 CCCGAG
BanI 919 GGTACC G^G[CT][GA]CC
2163 GGCACC
BcnI 105 CCCGG CC^[GC]GG
106 CCGGG
BseRI 443 GAGGAG GAGGAG(10/8)
2491 GAGGAG
BsoBI 105 CCCGGG C^[CT]CG[GA]G
210 CCCGAG
BspHI 768 TCATGA T^CATGA
2684 TCATGA
BspLU11I 644 ACATGT A^CATGT
3655 ACATGT
DraII 14 GGGTCCC [GA]G^G[ACGT]CC[CT]
3799 AGGACCT
EaeI 526 TGGCCA [CT]^GGCC[GA]
1977 TGGCCG
EcoO109I 14 GGGTCCC [GA]G^G[ACGT]CC[CT]
3799 AGGACCT
EcoT22I 421 ATGCAT ATGCA^T
3587 ATGCAT
HincII 3021 GTTGAC GT[CT]^[GA]AC
3562 GTTGAC
HindII 3021 GTTGAC GT[CT]^[GA]AC
3562 GTTGAC
MfeI 1502 CAATTG C^AATTG
2661 CAATTG
MunI 1502 CAATTG C^AATTG
2661 CAATTG
NciI 105 CCCGG CC^[GC]GG
106 CCGGG
NsiI 421 ATGCAT ATGCA^T
3587 ATGCAT
PflMI 532 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1577 CCAAAGTGTGG
PpuMI 14 GGGTCCC [GA]G^G[AT]CC[CT]
3799 AGGACCT
PvuII 180 CAGCTG CAG^CTG
2974 CAGCTG
RcaI 768 TCATGA T^CATGA
2684 TCATGA
SmlI 948 CTTGAG C^T[CT][GA]AG
2845 CTCAAG
SspI 1046 AATATT AAT^ATT
2396 AATATT
Van91I 532 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1577 CCAAAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaLI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I Cfr13I DdeI DpnII
DsaI EarI Eco81I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XmnI