##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AocI AscI Asp700I
Asp718I BbeI BcgI BclI BfrI
BglII BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsiWI BspEI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII CpoI DraI Eco81I
EcoT22I FbaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AgeI 1156 ACCGGT A^CCGGT
Alw44I 438 GTGCAC G^TGCAC
ApaLI 438 GTGCAC G^TGCAC
AseI 3203 ATTAAT AT^TAAT
AsnI 3203 ATTAAT AT^TAAT
AviII 3176 TGCGCA TGC^GCA
BamHI 2879 GGATCC G^GATCC
BbrPI 1470 CACGTG CAC^GTG
BsaAI 1470 CACGTG [CT]AC^GT[GA]
BsiEI 83 CGGCCG CG[GA][CT]^CG
BsmBI 966 GAGACG CGTCTC(1/5)
BspDI 192 ATCGAT AT^CGAT
BspHI 4585 TCATGA T^CATGA
BspLU11I 3021 ACATGT A^CATGT
ClaI 192 ATCGAT AT^CGAT
DraIII 1746 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 83 CGGCCG C^GGCCG
EclXI 83 CGGCCG C^GGCCG
Eco52I 83 CGGCCG C^GGCCG
EcoRI 1835 GAATTC G^AATTC
EcoRV 2037 GATATC GAT^ATC
FseI 84 GGCCGGCC GGCCGG^CC
FspI 3176 TGCGCA TGC^GCA
HgaI 738 GACGC GACGC(5/10)
NheI 2898 GCTAGC G^CTAGC
NspI 3021 ACATGT [GA]CATG^[CT]
PaeR7I 1336 CTCGAG C^TCGAG
PinAI 1156 ACCGGT A^CCGGT
PmaCI 1470 CACGTG CAC^GTG
PmlI 1470 CACGTG CAC^GTG
PshBI 3203 ATTAAT AT^TAAT
RcaI 4585 TCATGA T^CATGA
SapI 1241 GAAGAGC GCTCTTC(1/4)
XhoI 1336 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 1873 ACGTGT A^C[GA][CT]GT
3021 ACATGT
ApaI 305 GGGCCC GGGCC^C
3964 GGGCCC
AvrII 2676 CCTAGG C^CTAGG
3078 CCTAGG
BciVI 2466 GGATAC GTATCC(6/5)
4248 GGATAC
BglI 2933 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3076 GCCCTAGGGGC
BlnI 2676 CCTAGG C^CTAGG
3078 CCTAGG
Bsp1407I 1262 TGTACA T^GTACA
2575 TGTACA
BsrBI 278 GAGCGG CCGCTC(-3/-3)
3030 GAGCGG
BsrGI 1262 TGTACA T^GTACA
2575 TGTACA
BstAPI 3667 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3711 GCAGAGCCTGC
BstXI 2134 CCACCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3539 CCACGACATTGG
DrdI 1080 GACCGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1992 GACCCTGTGGTC
HincII 2922 GTTGAC GT[CT]^[GA]AC
4045 GTCAAC
HindII 2922 GTTGAC GT[CT]^[GA]AC
4045 GTCAAC
HindIII 3472 AAGCTT A^AGCTT
4230 AAGCTT
PflMI 1756 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2722 CCAGCAGGTGG
SexAI 634 ACCTGGT A^CC[AT]GGT
1813 ACCTGGT
SspBI 1262 TGTACA T^GTACA
2575 TGTACA
Van91I 1756 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2722 CCAGCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI Aor51HI ApoI
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco47III
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflFI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmaCI
XmaI