################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AocI AscI Asp700I Asp718I BbeI BcgI BclI BfrI BglII BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsiWI BspEI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CpoI DraI Eco81I EcoT22I FbaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AgeI 1156 ACCGGT A^CCGGT Alw44I 438 GTGCAC G^TGCAC ApaLI 438 GTGCAC G^TGCAC AseI 3203 ATTAAT AT^TAAT AsnI 3203 ATTAAT AT^TAAT AviII 3176 TGCGCA TGC^GCA BamHI 2879 GGATCC G^GATCC BbrPI 1470 CACGTG CAC^GTG BsaAI 1470 CACGTG [CT]AC^GT[GA] BsiEI 83 CGGCCG CG[GA][CT]^CG BsmBI 966 GAGACG CGTCTC(1/5) BspDI 192 ATCGAT AT^CGAT BspHI 4585 TCATGA T^CATGA BspLU11I 3021 ACATGT A^CATGT ClaI 192 ATCGAT AT^CGAT DraIII 1746 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 83 CGGCCG C^GGCCG EclXI 83 CGGCCG C^GGCCG Eco52I 83 CGGCCG C^GGCCG EcoRI 1835 GAATTC G^AATTC EcoRV 2037 GATATC GAT^ATC FseI 84 GGCCGGCC GGCCGG^CC FspI 3176 TGCGCA TGC^GCA HgaI 738 GACGC GACGC(5/10) NheI 2898 GCTAGC G^CTAGC NspI 3021 ACATGT [GA]CATG^[CT] PaeR7I 1336 CTCGAG C^TCGAG PinAI 1156 ACCGGT A^CCGGT PmaCI 1470 CACGTG CAC^GTG PmlI 1470 CACGTG CAC^GTG PshBI 3203 ATTAAT AT^TAAT RcaI 4585 TCATGA T^CATGA SapI 1241 GAAGAGC GCTCTTC(1/4) XhoI 1336 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 1873 ACGTGT A^C[GA][CT]GT 3021 ACATGT ApaI 305 GGGCCC GGGCC^C 3964 GGGCCC AvrII 2676 CCTAGG C^CTAGG 3078 CCTAGG BciVI 2466 GGATAC GTATCC(6/5) 4248 GGATAC BglI 2933 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3076 GCCCTAGGGGC BlnI 2676 CCTAGG C^CTAGG 3078 CCTAGG Bsp1407I 1262 TGTACA T^GTACA 2575 TGTACA BsrBI 278 GAGCGG CCGCTC(-3/-3) 3030 GAGCGG BsrGI 1262 TGTACA T^GTACA 2575 TGTACA BstAPI 3667 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3711 GCAGAGCCTGC BstXI 2134 CCACCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3539 CCACGACATTGG DrdI 1080 GACCGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1992 GACCCTGTGGTC HincII 2922 GTTGAC GT[CT]^[GA]AC 4045 GTCAAC HindII 2922 GTTGAC GT[CT]^[GA]AC 4045 GTCAAC HindIII 3472 AAGCTT A^AGCTT 4230 AAGCTT PflMI 1756 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2722 CCAGCAGGTGG SexAI 634 ACCTGGT A^CC[AT]GGT 1813 ACCTGGT SspBI 1262 TGTACA T^GTACA 2575 TGTACA Van91I 1756 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2722 CCAGCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AfaI AhdI AluI AlwI AlwNI Aor51HI ApoI AspEI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco47III EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflFI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmaCI XmaI