##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI AspEI
AvaI AviII AvrII BbeI BbvCI
BfrI BglI BlnI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI FseI FspI Hin1I
KasI KspI MamI MfeI MluI
MroI MunI MvnI NarI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3377 GGTACC G^GTACC
Aor51HI 802 AGCGCT AGC^GCT
Asp700I 1578 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3377 GGTACC G^GTACC
AspI 3194 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 596 TGGCCA TGG^CCA
BamHI 3170 GGATCC G^GATCC
BanI 3377 GGTACC G^G[CT][GA]CC
BbrPI 592 CACGTG CAC^GTG
BcgI 2605 GCAAGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2419 GGATAC GTATCC(6/5)
BclI 1185 TGATCA T^GATCA
BglII 1563 AGATCT A^GATCT
BseRI 2549 GAGGAG GAGGAG(10/8)
BspLU11I 3639 ACATGT A^CATGT
BspMI 1042 ACCTGC ACCTGC(4/8)
BstEII 673 GGTGACC G^GT[ACGT]ACC
BstPI 673 GGTGACC G^GT[ACGT]ACC
CfoI 803 GCGC GCG^C
Eco47III 802 AGCGCT AGC^GCT
EcoO65I 673 GGTGACC G^GT[ACGT]ACC
FauI 51 CCCGC CCCGC(4/6)
FbaI 1185 TGATCA T^GATCA
HaeII 802 AGCGCT [GA]GCGC^[CT]
HgaI 1917 GCGTC GACGC(5/10)
HhaI 803 GCGC GCG^C
HinP1I 803 GCGC G^CGC
HindIII 3829 AAGCTT A^AGCTT
KpnI 3377 GGTACC GGTAC^C
MluNI 596 TGGCCA TGG^CCA
MscI 596 TGGCCA TGG^CCA
NaeI 1673 GCCGGC GCC^GGC
NcoI 599 CCATGG C^CATGG
NgoMIV 1673 GCCGGC G^CCGGC
PflFI 3194 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 762 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 592 CACGTG CAC^GTG
PmlI 592 CACGTG CAC^GTG
PshAI 434 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4025 GAGCTC GAGCT^C
SalI 1570 GTCGAC G^TCGAC
SapI 395 GAAGAGC GCTCTTC(1/4)
SphI 1323 GCATGC GCATG^C
StuI 640 AGGCCT AGG^CCT
Tth111I 3194 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 762 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 379 CCAACTATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1578 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 1895 ACGTGT A^C[GA][CT]GT
3639 ACATGT
AspHI 2225 GTGCTC G[AT]GC[AT]^C
4025 GAGCTC
BcnI 321 CCCGG CC^[GC]GG
1722 CCCGG
BsaI 1253 GAGACC GGTCTC(1/5)
1467 GAGACC
BsiHKAI 2225 GTGCTC G[AT]GC[AT]^C
4025 GAGCTC
BsmI 352 GAATGC GAATGC(1/-1)
3836 GCATTC
Bsp1407I 2432 TGTACA T^GTACA
3155 TGTACA
BsrDI 2900 GCAATG GCAATG(2/0)
3959 GCAATG
BsrFI 484 GCCGGT [GA]^CCGG[CT]
1673 GCCGGC
BsrGI 2432 TGTACA T^GTACA
3155 TGTACA
BstXI 1985 CCACCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2322 CCAACCCCCTGG
Cfr10I 484 GCCGGT [GA]^CCGG[CT]
1673 GCCGGC
EaeI 596 TGGCCA [CT]^GGCC[GA]
1834 CGGCCA
HpaI 684 GTTAAC GTT^AAC
3007 GTTAAC
NciI 321 CCCGG CC^[GC]GG
1722 CCCGG
SmlI 134 CTCAAG C^T[CT][GA]AG
3208 CTCAAG
SspBI 2432 TGTACA T^GTACA
3155 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaII
BanII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII