################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AgeI Alw44I AocI Aor51HI ApaLI AscI Asp718I AviII BbeI BcgI BlpI Bpu1102I BsaBI BsiWI BspLU11I BsrFI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FseI FspI HindIII KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PvuI SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 1390 TCCGGA T^CCGGA AclI 3264 AACGTT AA^CGTT AflII 3691 CTTAAG C^TTAAG AflIII 2293 ACACGT A^C[GA][CT]GT AseI 3307 ATTAAT AT^TAAT AsnI 3307 ATTAAT AT^TAAT BamHI 1976 GGATCC G^GATCC BclI 959 TGATCA T^GATCA BfrI 3691 CTTAAG C^TTAAG BglII 904 AGATCT A^GATCT BseAI 1390 TCCGGA T^CCGGA BsmBI 1540 CGTCTC CGTCTC(1/5) Bsp1407I 3115 TGTACA T^GTACA BspEI 1390 TCCGGA T^CCGGA BsrGI 3115 TGTACA T^GTACA BssSI 2082 CTCGTG CACGAG(-5/-1) CpoI 18 CGGACCG CG^G[AT]CCG DrdI 1380 GACATTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 459 GATATC GAT^ATC FbaI 959 TGATCA T^GATCA HpaI 3881 GTTAAC GTT^AAC MroI 1390 TCCGGA T^CCGGA NdeI 2658 CATATG CA^TATG NruI 1166 TCGCGA TCG^CGA PshBI 3307 ATTAAT AT^TAAT Psp1406I 3264 AACGTT AA^CGTT RsrII 18 CGGACCG CG^G[AT]CCG SapI 3915 GAAGAGC GCTCTTC(1/4) SbfI 689 CCTGCAGG CCTGCA^GG ScaI 991 AGTACT AGT^ACT SmaI 188 CCCGGG CCC^GGG SphI 4037 GCATGC GCATG^C Sse8387I 689 CCTGCAGG CCTGCA^GG SspBI 3115 TGTACA T^GTACA StuI 2531 AGGCCT AGG^CCT XmaCI 188 CCCGGG C^CCGGG XmaI 188 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 1965 GTAGAC GT^[AC][GT]AC 4098 GTCTAC AhdI 644 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 813 GACCGGGTGTC Asp700I 296 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1284 GAAAGCATTC AspEI 644 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 813 GACCGGGTGTC AspI 2061 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2879 GACTGGGTC BbrPI 7 CACGTG CAC^GTG 1095 CACGTG BbvCI 1490 CCTCAGC CCTCAGC(-5/-2) 672 GCTGAGG BciVI 1535 GGATAC GTATCC(6/5) 3798 GTATCC BglI 938 GCCCGTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2682 GCCCCCATGGC BsaI 1170 GAGACC GGTCTC(1/5) 3909 GAGACC BsaWI 1390 TCCGGA [AT]^CCGG[AT] 1873 ACCGGA BsiEI 701 CGGTCG CG[GA][CT]^CG 1871 CGACCG BsmI 4226 GAATGC GAATGC(1/-1) 1288 GCATTC BspDI 1635 ATCGAT AT^CGAT 1803 ATCGAT BspHI 865 TCATGA T^CATGA 2842 TCATGA BsrBI 226 CCGCTC CCGCTC(-3/-3) 2256 GAGCGG BstAPI 1215 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1349 GCAGCAGGTGC BstEII 641 GGTGACC G^GT[ACGT]ACC 3937 GGTGACC BstPI 641 GGTGACC G^GT[ACGT]ACC 3937 GGTGACC ClaI 1635 ATCGAT AT^CGAT 1803 ATCGAT DraI 3336 TTTAAA TTT^AAA 4076 TTTAAA DsaI 1672 CCATGG C^C[GA][CT]GG 2686 CCATGG Eam1105I 644 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 813 GACCGGGTGTC EarI 1770 CTCTTC CTCTTC(1/4) 3915 GAAGAG EcoO65I 641 GGTGACC G^GT[ACGT]ACC 3937 GGTGACC HaeII 985 AGCGCC [GA]GCGC^[CT] 1931 AGCGCC HincII 3881 GTTAAC GT[CT]^[GA]AC 4177 GTCAAC HindII 3881 GTTAAC GT[CT]^[GA]AC 4177 GTCAAC Ksp632I 1770 CTCTTC CTCTTC(1/4) 3915 GAAGAG NcoI 1672 CCATGG C^CATGG 2686 CCATGG NheI 668 GCTAGC G^CTAGC 2896 GCTAGC PflFI 2061 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2879 GACTGGGTC PmaCI 7 CACGTG CAC^GTG 1095 CACGTG PmlI 7 CACGTG CAC^GTG 1095 CACGTG PstI 690 CTGCAG CTGCA^G 2031 CTGCAG RcaI 865 TCATGA T^CATGA 2842 TCATGA SacI 423 GAGCTC GAGCT^C 2229 GAGCTC Tth111I 2061 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2879 GACTGGGTC XmnI 296 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1284 GAAAGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII