##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AgeI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AviII
BbeI BcgI BlpI Bpu1102I BsaBI
BsiWI BspLU11I BsrFI BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FseI FspI HindIII
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NotI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 1390 TCCGGA T^CCGGA
AclI 3264 AACGTT AA^CGTT
AflII 3691 CTTAAG C^TTAAG
AflIII 2293 ACACGT A^C[GA][CT]GT
AseI 3307 ATTAAT AT^TAAT
AsnI 3307 ATTAAT AT^TAAT
BamHI 1976 GGATCC G^GATCC
BclI 959 TGATCA T^GATCA
BfrI 3691 CTTAAG C^TTAAG
BglII 904 AGATCT A^GATCT
BseAI 1390 TCCGGA T^CCGGA
BsmBI 1540 CGTCTC CGTCTC(1/5)
Bsp1407I 3115 TGTACA T^GTACA
BspEI 1390 TCCGGA T^CCGGA
BsrGI 3115 TGTACA T^GTACA
BssSI 2082 CTCGTG CACGAG(-5/-1)
CpoI 18 CGGACCG CG^G[AT]CCG
DrdI 1380 GACATTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 459 GATATC GAT^ATC
FbaI 959 TGATCA T^GATCA
HpaI 3881 GTTAAC GTT^AAC
MroI 1390 TCCGGA T^CCGGA
NdeI 2658 CATATG CA^TATG
NruI 1166 TCGCGA TCG^CGA
PshBI 3307 ATTAAT AT^TAAT
Psp1406I 3264 AACGTT AA^CGTT
RsrII 18 CGGACCG CG^G[AT]CCG
SapI 3915 GAAGAGC GCTCTTC(1/4)
SbfI 689 CCTGCAGG CCTGCA^GG
ScaI 991 AGTACT AGT^ACT
SmaI 188 CCCGGG CCC^GGG
SphI 4037 GCATGC GCATG^C
Sse8387I 689 CCTGCAGG CCTGCA^GG
SspBI 3115 TGTACA T^GTACA
StuI 2531 AGGCCT AGG^CCT
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1965 GTAGAC GT^[AC][GT]AC
4098 GTCTAC
AhdI 644 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
813 GACCGGGTGTC
Asp700I 296 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1284 GAAAGCATTC
AspEI 644 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
813 GACCGGGTGTC
AspI 2061 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2879 GACTGGGTC
BbrPI 7 CACGTG CAC^GTG
1095 CACGTG
BbvCI 1490 CCTCAGC CCTCAGC(-5/-2)
672 GCTGAGG
BciVI 1535 GGATAC GTATCC(6/5)
3798 GTATCC
BglI 938 GCCCGTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2682 GCCCCCATGGC
BsaI 1170 GAGACC GGTCTC(1/5)
3909 GAGACC
BsaWI 1390 TCCGGA [AT]^CCGG[AT]
1873 ACCGGA
BsiEI 701 CGGTCG CG[GA][CT]^CG
1871 CGACCG
BsmI 4226 GAATGC GAATGC(1/-1)
1288 GCATTC
BspDI 1635 ATCGAT AT^CGAT
1803 ATCGAT
BspHI 865 TCATGA T^CATGA
2842 TCATGA
BsrBI 226 CCGCTC CCGCTC(-3/-3)
2256 GAGCGG
BstAPI 1215 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1349 GCAGCAGGTGC
BstEII 641 GGTGACC G^GT[ACGT]ACC
3937 GGTGACC
BstPI 641 GGTGACC G^GT[ACGT]ACC
3937 GGTGACC
ClaI 1635 ATCGAT AT^CGAT
1803 ATCGAT
DraI 3336 TTTAAA TTT^AAA
4076 TTTAAA
DsaI 1672 CCATGG C^C[GA][CT]GG
2686 CCATGG
Eam1105I 644 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
813 GACCGGGTGTC
EarI 1770 CTCTTC CTCTTC(1/4)
3915 GAAGAG
EcoO65I 641 GGTGACC G^GT[ACGT]ACC
3937 GGTGACC
HaeII 985 AGCGCC [GA]GCGC^[CT]
1931 AGCGCC
HincII 3881 GTTAAC GT[CT]^[GA]AC
4177 GTCAAC
HindII 3881 GTTAAC GT[CT]^[GA]AC
4177 GTCAAC
Ksp632I 1770 CTCTTC CTCTTC(1/4)
3915 GAAGAG
NcoI 1672 CCATGG C^CATGG
2686 CCATGG
NheI 668 GCTAGC G^CTAGC
2896 GCTAGC
PflFI 2061 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2879 GACTGGGTC
PmaCI 7 CACGTG CAC^GTG
1095 CACGTG
PmlI 7 CACGTG CAC^GTG
1095 CACGTG
PstI 690 CTGCAG CTGCA^G
2031 CTGCAG
RcaI 865 TCATGA T^CATGA
2842 TCATGA
SacI 423 GAGCTC GAGCT^C
2229 GAGCTC
Tth111I 2061 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2879 GACTGGGTC
XmnI 296 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1284 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII