##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII Aor51HI
AscI AseI AsnI Asp718I AvrII
BbeI BcgI BciVI BclI BfrI
BlnI BsiWI Bsp1407I BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Eco47III EcoO65I EcoT22I
FbaI FseI HaeII HpaI KasI
KpnI MfeI MunI NarI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 721 TCCGGA T^CCGGA
AcyI 939 GACGCC G[GA]^CG[CT]C
AgeI 2583 ACCGGT A^CCGGT
AhdI 4496 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1505 GTGCAC G^TGCAC
ApaLI 1505 GTGCAC G^TGCAC
Asp700I 1073 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4496 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1548 CTCGAG C^[CT]CG[GA]G
AviII 580 TGCGCA TGC^GCA
BalI 2351 TGGCCA TGG^CCA
BbrPI 2724 CACGTG CAC^GTG
BsaBI 983 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 939 GACGCC G[GA]^CG[CT]C
BseAI 721 TCCGGA T^CCGGA
BsiEI 3 CGGCCG CG[GA][CT]^CG
BsmBI 519 GAGACG CGTCTC(1/5)
BsoBI 1548 CTCGAG C^[CT]CG[GA]G
BspDI 891 ATCGAT AT^CGAT
BspEI 721 TCCGGA T^CCGGA
BspHI 1816 TCATGA T^CATGA
ClaI 891 ATCGAT AT^CGAT
CpoI 83 CGGACCG CG^G[AT]CCG
DraI 4093 TTTAAA TTT^AAA
DrdI 1803 GACCATAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3 CGGCCG C^GGCCG
Eam1105I 4496 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3 CGGCCG C^GGCCG
Eco52I 3 CGGCCG C^GGCCG
EcoRI 4344 GAATTC G^AATTC
EcoRV 480 GATATC GAT^ATC
FspI 580 TGCGCA TGC^GCA
Hin1I 939 GACGCC G[GA]^CG[CT]C
KspI 241 CCGCGG CCGC^GG
MamI 983 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4477 ACGCGT A^CGCGT
MluNI 2351 TGGCCA TGG^CCA
MroI 721 TCCGGA T^CCGGA
MscI 2351 TGGCCA TGG^CCA
NcoI 2718 CCATGG C^CATGG
NdeI 3075 CATATG CA^TATG
NheI 2343 GCTAGC G^CTAGC
NotI 2 GCGGCCGC GC^GGCCGC
NspI 2110 GCATGT [GA]CATG^[CT]
PaeR7I 1548 CTCGAG C^TCGAG
PinAI 2583 ACCGGT A^CCGGT
PmaCI 2724 CACGTG CAC^GTG
PmlI 2724 CACGTG CAC^GTG
RcaI 1816 TCATGA T^CATGA
RsrII 83 CGGACCG CG^G[AT]CCG
SacII 241 CCGCGG CCGC^GG
SalI 265 GTCGAC G^TCGAC
SpeI 3282 ACTAGT A^CTAGT
StuI 740 AGGCCT AGG^CCT
XbaI 3474 TCTAGA T^CTAGA
XhoI 1548 CTCGAG C^TCGAG
XmnI 1073 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 265 GTCGAC GT^[AC][GT]AC
2494 GTAGAC
AcsI 1889 GAATTT [GA]^AATT[CT]
4344 GAATTC
AflIII 2725 ACGTGT A^C[GA][CT]GT
4477 ACGCGT
AocI 1929 CCTCAGG CC^T[ACGT]AGG
2935 CCTGAGG
ApaI 2370 GGGCCC GGGCC^C
4418 GGGCCC
ApoI 1889 GAATTT [GA]^AATT[CT]
4344 GAATTC
AspI 1668 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2927 GACATGGTC
BamHI 982 GGATCC G^GATCC
4567 GGATCC
BbsI 869 GAAGAC GAAGAC(2/6)
1812 GTCTTC
BglI 237 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4354 GCCGCTGAGGC
BlpI 3530 GCTGAGC GC^T[ACGT]AGC
4409 GCTGAGC
Bpu1102I 3530 GCTGAGC GC^T[ACGT]AGC
4409 GCTGAGC
BpuAI 869 GAAGAC GAAGAC(2/6)
1812 GTCTTC
BsaAI 2724 CACGTG [CT]AC^GT[GA]
3022 CACGTA
BsgI 3390 GTGCAG GTGCAG(16/14)
4329 GTGCAG
BsmI 757 GAATGC GAATGC(1/-1)
4014 GAATGC
Bsu36I 1929 CCTCAGG CC^T[ACGT]AGG
2935 CCTGAGG
CelII 3530 GCTGAGC GC^T[ACGT]AGC
4409 GCTGAGC
Eco81I 1929 CCTCAGG CC^T[ACGT]AGG
2935 CCTGAGG
EcoNI 2571 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3457 CCTGAGACAGG
HincII 265 GTCGAC GT[CT]^[GA]AC
825 GTCAAC
HindII 265 GTCGAC GT[CT]^[GA]AC
825 GTCAAC
MslI 1988 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2045 CATACCAATG
NaeI 2485 GCCGGC GCC^GGC
3101 GCCGGC
NgoMIV 2485 GCCGGC G^CCGGC
3101 GCCGGC
PflFI 1668 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2927 GACATGGTC
PflMI 2232 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4038 CCATTGTGTGG
SacI 1944 GAGCTC GAGCT^C
2092 GAGCTC
SexAI 2462 ACCAGGT A^CC[AT]GGT
3768 ACCAGGT
Tth111I 1668 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2927 GACATGGTC
Van91I 2232 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4038 CCATTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII