################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccIII AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AvrII BbeI BcgI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII BstBI BstEII BstPI CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I FseI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1292 GACGTC GACGT^C AclI 485 AACGTT AA^CGTT AcyI 1292 GACGTC G[GA]^CG[CT]C AflII 2083 CTTAAG C^TTAAG AhdI 3888 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2780 GTGCAC G^TGCAC AocI 101 CCTGAGG CC^T[ACGT]AGG ApaLI 2780 GTGCAC G^TGCAC Asp700I 692 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3888 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1115 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3972 GGATCC G^GATCC BanI 4429 GGCACC G^G[CT][GA]CC BciVI 1101 GGATAC GTATCC(6/5) BclI 1230 TGATCA T^GATCA BfrI 2083 CTTAAG C^TTAAG BglI 1872 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1292 GACGTC G[GA]^CG[CT]C BsaWI 3969 ACCGGA [AT]^CCGG[AT] BspHI 1617 TCATGA T^CATGA BssSI 680 CACGAG CACGAG(-5/-1) Bst1107I 88 GTATAC GTA^TAC BstZ17I 88 GTATAC GTA^TAC Bsu36I 101 CCTGAGG CC^T[ACGT]AGG DraI 4479 TTTAAA TTT^AAA DraIII 3950 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3888 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 101 CCTGAGG CC^T[ACGT]AGG EcoRV 2384 GATATC GAT^ATC EcoT22I 2435 ATGCAT ATGCA^T FbaI 1230 TGATCA T^GATCA HaeII 3660 AGCGCC [GA]GCGC^[CT] Hin1I 1292 GACGTC G[GA]^CG[CT]C HincII 1072 GTCAAC GT[CT]^[GA]AC HindII 1072 GTCAAC GT[CT]^[GA]AC HindIII 2801 AAGCTT A^AGCTT NcoI 2259 CCATGG C^CATGG NsiI 2435 ATGCAT ATGCA^T PflFI 1115 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 485 AACGTT AA^CGTT RcaI 1617 TCATGA T^CATGA ScaI 2307 AGTACT AGT^ACT SmaI 1779 CCCGGG CCC^GGG Tth111I 1115 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1779 CCCGGG C^CCGGG XmaI 1779 CCCGGG C^CCGGG XmnI 692 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 88 GTATAC GT^[AC][GT]AC 1481 GTCTAC AcsI 311 GAATTC [GA]^AATT[CT] 347 GAATTC ApoI 311 GAATTC [GA]^AATT[CT] 347 GAATTC AvaI 740 CCCGAG C^[CT]CG[GA]G 1779 CCCGGG BalI 1311 TGGCCA TGG^CCA 3114 TGGCCA BbrPI 3871 CACGTG CAC^GTG 3921 CACGTG BsmI 4168 GAATGC GAATGC(1/-1) 3747 GCATTC BsoBI 740 CCCGAG C^[CT]CG[GA]G 1779 CCCGGG BspLU11I 2253 ACATGT A^CATGT 3854 ACATGT BsrBI 327 CCGCTC CCGCTC(-3/-3) 1439 GAGCGG BsrDI 1828 CATTGC GCAATG(2/0) 1465 GCAATG EcoRI 311 GAATTC G^AATTC 347 GAATTC MluNI 1311 TGGCCA TGG^CCA 3114 TGGCCA MscI 1311 TGGCCA TGG^CCA 3114 TGGCCA PflMI 1297 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3643 CCAGCTGATGG PmaCI 3871 CACGTG CAC^GTG 3921 CACGTG PmlI 3871 CACGTG CAC^GTG 3921 CACGTG SacI 716 GAGCTC GAGCT^C 2195 GAGCTC SbfI 2134 CCTGCAGG CCTGCA^GG 3589 CCTGCAGG Sse8387I 2134 CCTGCAGG CCTGCA^GG 3589 CCTGCAGG Van91I 1297 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3643 CCAGCTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaII AviII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII