##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AvrII
BbeI BcgI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BstBI BstEII BstPI CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I FseI HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1292 GACGTC GACGT^C
AclI 485 AACGTT AA^CGTT
AcyI 1292 GACGTC G[GA]^CG[CT]C
AflII 2083 CTTAAG C^TTAAG
AhdI 3888 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2780 GTGCAC G^TGCAC
AocI 101 CCTGAGG CC^T[ACGT]AGG
ApaLI 2780 GTGCAC G^TGCAC
Asp700I 692 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3888 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1115 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3972 GGATCC G^GATCC
BanI 4429 GGCACC G^G[CT][GA]CC
BciVI 1101 GGATAC GTATCC(6/5)
BclI 1230 TGATCA T^GATCA
BfrI 2083 CTTAAG C^TTAAG
BglI 1872 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1292 GACGTC G[GA]^CG[CT]C
BsaWI 3969 ACCGGA [AT]^CCGG[AT]
BspHI 1617 TCATGA T^CATGA
BssSI 680 CACGAG CACGAG(-5/-1)
Bst1107I 88 GTATAC GTA^TAC
BstZ17I 88 GTATAC GTA^TAC
Bsu36I 101 CCTGAGG CC^T[ACGT]AGG
DraI 4479 TTTAAA TTT^AAA
DraIII 3950 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3888 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 101 CCTGAGG CC^T[ACGT]AGG
EcoRV 2384 GATATC GAT^ATC
EcoT22I 2435 ATGCAT ATGCA^T
FbaI 1230 TGATCA T^GATCA
HaeII 3660 AGCGCC [GA]GCGC^[CT]
Hin1I 1292 GACGTC G[GA]^CG[CT]C
HincII 1072 GTCAAC GT[CT]^[GA]AC
HindII 1072 GTCAAC GT[CT]^[GA]AC
HindIII 2801 AAGCTT A^AGCTT
NcoI 2259 CCATGG C^CATGG
NsiI 2435 ATGCAT ATGCA^T
PflFI 1115 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 485 AACGTT AA^CGTT
RcaI 1617 TCATGA T^CATGA
ScaI 2307 AGTACT AGT^ACT
SmaI 1779 CCCGGG CCC^GGG
Tth111I 1115 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1779 CCCGGG C^CCGGG
XmaI 1779 CCCGGG C^CCGGG
XmnI 692 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 88 GTATAC GT^[AC][GT]AC
1481 GTCTAC
AcsI 311 GAATTC [GA]^AATT[CT]
347 GAATTC
ApoI 311 GAATTC [GA]^AATT[CT]
347 GAATTC
AvaI 740 CCCGAG C^[CT]CG[GA]G
1779 CCCGGG
BalI 1311 TGGCCA TGG^CCA
3114 TGGCCA
BbrPI 3871 CACGTG CAC^GTG
3921 CACGTG
BsmI 4168 GAATGC GAATGC(1/-1)
3747 GCATTC
BsoBI 740 CCCGAG C^[CT]CG[GA]G
1779 CCCGGG
BspLU11I 2253 ACATGT A^CATGT
3854 ACATGT
BsrBI 327 CCGCTC CCGCTC(-3/-3)
1439 GAGCGG
BsrDI 1828 CATTGC GCAATG(2/0)
1465 GCAATG
EcoRI 311 GAATTC G^AATTC
347 GAATTC
MluNI 1311 TGGCCA TGG^CCA
3114 TGGCCA
MscI 1311 TGGCCA TGG^CCA
3114 TGGCCA
PflMI 1297 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3643 CCAGCTGATGG
PmaCI 3871 CACGTG CAC^GTG
3921 CACGTG
PmlI 3871 CACGTG CAC^GTG
3921 CACGTG
SacI 716 GAGCTC GAGCT^C
2195 GAGCTC
SbfI 2134 CCTGCAGG CCTGCA^GG
3589 CCTGCAGG
Sse8387I 2134 CCTGCAGG CCTGCA^GG
3589 CCTGCAGG
Van91I 1297 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3643 CCAGCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII AviII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII