##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI BamHI BcgI BfrI
BglI BglII BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI BstEII BstPI BstXI
CelII ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I FseI
HpaI KspI MamI MfeI MluI
MroI MunI NheI NotI NruI
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 204 GGTACC G^GTACC
AccII 1899 CGCG CG^CG
AocI 2551 CCTTAGG CC^T[ACGT]AGG
AseI 4174 ATTAAT AT^TAAT
AsnI 4174 ATTAAT AT^TAAT
Asp700I 4087 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 204 GGTACC G^GTACC
AspI 2039 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4616 TGCGCA TGC^GCA
AvrII 2816 CCTAGG C^CTAGG
BbeI 1213 GGCGCC GGCGC^C
BbvCI 1259 GCTGAGG CCTCAGC(-5/-2)
BciVI 3195 GTATCC GTATCC(6/5)
BclI 351 TGATCA T^GATCA
BlnI 2816 CCTAGG C^CTAGG
BsaWI 1955 ACCGGA [AT]^CCGG[AT]
BsmBI 2419 CGTCTC CGTCTC(1/5)
BspLU11I 1146 ACATGT A^CATGT
BspMI 533 ACCTGC ACCTGC(4/8)
BsrBI 767 GAGCGG CCGCTC(-3/-3)
BstAPI 249 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1899 CGCG CG^CG
BstYI 4491 AGATCC [GA]^GATC[CT]
Bsu36I 2551 CCTTAGG CC^T[ACGT]AGG
DsaI 4449 CCATGG C^C[GA][CT]GG
Eco81I 2551 CCTTAGG CC^T[ACGT]AGG
EcoRI 611 GAATTC G^AATTC
EcoRV 4894 GATATC GAT^ATC
FbaI 351 TGATCA T^GATCA
FspI 4616 TGCGCA TGC^GCA
HaeII 1213 GGCGCC [GA]GCGC^[CT]
HincII 1588 GTCAAC GT[CT]^[GA]AC
HindII 1588 GTCAAC GT[CT]^[GA]AC
HindIII 4287 AAGCTT A^AGCTT
KasI 1213 GGCGCC G^GCGCC
KpnI 204 GGTACC GGTAC^C
MflI 4491 AGATCC [GA]^GATC[CT]
MvnI 1899 CGCG CG^CG
NaeI 3757 GCCGGC GCC^GGC
NarI 1213 GGCGCC GG^CGCC
NcoI 4449 CCATGG C^CATGG
NdeI 3401 CATATG CA^TATG
NgoMIV 3757 GCCGGC G^CCGGC
PflFI 2039 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 503 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4174 ATTAAT AT^TAAT
SacI 344 GAGCTC GAGCT^C
SapI 3556 GAAGAGC GCTCTTC(1/4)
SfoI 1213 GGCGCC GGC^GCC
SphI 811 GCATGC GCATG^C
Tth111I 2039 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4804 TCTAGA T^CTAGA
XhoII 4491 AGATCC [GA]^GATC[CT]
XmnI 4087 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 2342 GATCC GGATC(4/5)
4492 GATCC
AlwNI 1485 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
2850 CAGTAGCTG
BalI 646 TGGCCA TGG^CCA
999 TGGCCA
BsmI 1768 GAATGC GAATGC(1/-1)
4776 GAATGC
Bsp1407I 759 TGTACA T^GTACA
3218 TGTACA
BspHI 3773 TCATGA T^CATGA
3835 TCATGA
BsrFI 207 ACCGGC [GA]^CCGG[CT]
3757 GCCGGC
BsrGI 759 TGTACA T^GTACA
3218 TGTACA
Bst1107I 4151 GTATAC GTA^TAC
4244 GTATAC
BstBI 1644 TTCGAA TT^CGAA
4886 TTCGAA
BstZ17I 4151 GTATAC GTA^TAC
4244 GTATAC
Cfr10I 207 ACCGGC [GA]^CCGG[CT]
3757 GCCGGC
DraIII 1728 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3709 CACCTTGTG
EcoNI 644 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4883 CCTTTCGAAGG
MluNI 646 TGGCCA TGG^CCA
999 TGGCCA
MscI 646 TGGCCA TGG^CCA
999 TGGCCA
MslI 1439 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4752 CACTGTGATG
NspV 1644 TTCGAA TT^CGAA
4886 TTCGAA
RcaI 3773 TCATGA T^CATGA
3835 TCATGA
SfuI 1644 TTCGAA TT^CGAA
4886 TTCGAA
SmaI 27 CCCGGG CCC^GGG
1235 CCCGGG
SspBI 759 TGTACA T^GTACA
3218 TGTACA
XmaCI 27 CCCGGG C^CCGGG
1235 CCCGGG
XmaI 27 CCCGGG C^CCGGG
1235 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I ApaI
ApaLI ApoI AspEI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI Eam1105I EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI