################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AccIII AclI AcsI AgeI ApoI AscI AspI BbrPI BcgI BclI BsaBI BseAI BsiWI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EcoRI EcoRV EcoT22I FbaI FseI HpaI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SalI SfiI SfuI SgrAI SnaBI SspI SwaI TaqI Tth111I TthHB8I XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 4527 GACGTC GACGT^C AccII 101 CGCG CG^CG AflII 859 CTTAAG C^TTAAG AflIII 1488 ACATGT A^C[GA][CT]GT AhdI 3271 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3735 GTGCAC G^TGCAC Aor51HI 635 AGCGCT AGC^GCT ApaLI 3735 GTGCAC G^TGCAC AseI 2583 ATTAAT AT^TAAT AsnI 2583 ATTAAT AT^TAAT Asp700I 9 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3271 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 3113 CCCGGG C^[CT]CG[GA]G AvrII 1808 CCTAGG C^CTAGG BfrI 859 CTTAAG C^TTAAG BlnI 1808 CCTAGG C^CTAGG BlpI 4461 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4461 GCTGAGC GC^T[ACGT]AGC BsaAI 3140 TACGTG [CT]AC^GT[GA] BsaWI 3513 TCCGGT [AT]^CCGG[AT] BsiEI 33 CGGCCG CG[GA][CT]^CG BsmBI 518 GAGACG CGTCTC(1/5) BsmI 619 GAATGC GAATGC(1/-1) BsoBI 3113 CCCGGG C^[CT]CG[GA]G Bsp1407I 3581 TGTACA T^GTACA BspLU11I 1488 ACATGT A^CATGT BsrGI 3581 TGTACA T^GTACA BstEII 1684 GGTTACC G^GT[ACGT]ACC BstPI 1684 GGTTACC G^GT[ACGT]ACC BstUI 101 CGCG CG^CG CelII 4461 GCTGAGC GC^T[ACGT]AGC EagI 33 CGGCCG C^GGCCG Eam1105I 3271 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 33 CGGCCG C^GGCCG Eco47III 635 AGCGCT AGC^GCT Eco52I 33 CGGCCG C^GGCCG EcoO65I 1684 GGTTACC G^GT[ACGT]ACC HincII 3526 GTCAAC GT[CT]^[GA]AC HindII 3526 GTCAAC GT[CT]^[GA]AC HindIII 862 AAGCTT A^AGCTT KspI 100 CCGCGG CCGC^GG MvnI 101 CGCG CG^CG NcoI 808 CCATGG C^CATGG PshBI 2583 ATTAAT AT^TAAT SacII 100 CCGCGG CCGC^GG SbfI 4595 CCTGCAGG CCTGCA^GG ScaI 2428 AGTACT AGT^ACT SexAI 4127 ACCAGGT A^CC[AT]GGT SmaI 3113 CCCGGG CCC^GGG SpeI 3548 ACTAGT A^CTAGT SphI 1222 GCATGC GCATG^C Sse8387I 4595 CCTGCAGG CCTGCA^GG SspBI 3581 TGTACA T^GTACA XbaI 1721 TCTAGA T^CTAGA XmaCI 3113 CCCGGG C^CCGGG XmaI 3113 CCCGGG C^CCGGG XmnI 9 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Acc65I 3795 GGTACC G^GTACC 4138 GGTACC Asp718I 3795 GGTACC G^GTACC 4138 GGTACC AviII 1023 TGCGCA TGC^GCA 2700 TGCGCA BalI 891 TGGCCA TGG^CCA 3376 TGGCCA BbeI 497 GGCGCC GGCGC^C 677 GGCGCC BbsI 1309 GAAGAC GAAGAC(2/6) 3842 GAAGAC BciVI 1462 GGATAC GTATCC(6/5) 1670 GGATAC BpuAI 1309 GAAGAC GAAGAC(2/6) 3842 GAAGAC BsrBI 604 GAGCGG CCGCTC(-3/-3) 1559 GAGCGG DraIII 2964 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3241 CACTCTGTG DrdI 75 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3230 GACACAGGGGTC FauI 50 GCGGG CCCGC(4/6) 69 GCGGG FspI 1023 TGCGCA TGC^GCA 2700 TGCGCA KasI 497 GGCGCC G^GCGCC 677 GGCGCC KpnI 3795 GGTACC GGTAC^C 4138 GGTACC MluNI 891 TGGCCA TGG^CCA 3376 TGGCCA MscI 891 TGGCCA TGG^CCA 3376 TGGCCA NaeI 31 GCCGGC GCC^GGC 1218 GCCGGC NarI 497 GGCGCC GG^CGCC 677 GGCGCC NgoMIV 31 GCCGGC G^CCGGC 1218 GCCGGC NheI 4076 GCTAGC G^CTAGC 4274 GCTAGC PshAI 323 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2013 GACAGCTGTC SfoI 497 GGCGCC GGC^GCC 677 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaII BamHI BanI BanII BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII