##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AcsI AgeI ApoI
AscI AspI BbrPI BcgI BclI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV EcoT22I FbaI FseI HpaI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SfiI SfuI SgrAI
SnaBI SspI SwaI TaqI Tth111I
TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 4527 GACGTC GACGT^C
AccII 101 CGCG CG^CG
AflII 859 CTTAAG C^TTAAG
AflIII 1488 ACATGT A^C[GA][CT]GT
AhdI 3271 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3735 GTGCAC G^TGCAC
Aor51HI 635 AGCGCT AGC^GCT
ApaLI 3735 GTGCAC G^TGCAC
AseI 2583 ATTAAT AT^TAAT
AsnI 2583 ATTAAT AT^TAAT
Asp700I 9 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3271 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3113 CCCGGG C^[CT]CG[GA]G
AvrII 1808 CCTAGG C^CTAGG
BfrI 859 CTTAAG C^TTAAG
BlnI 1808 CCTAGG C^CTAGG
BlpI 4461 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4461 GCTGAGC GC^T[ACGT]AGC
BsaAI 3140 TACGTG [CT]AC^GT[GA]
BsaWI 3513 TCCGGT [AT]^CCGG[AT]
BsiEI 33 CGGCCG CG[GA][CT]^CG
BsmBI 518 GAGACG CGTCTC(1/5)
BsmI 619 GAATGC GAATGC(1/-1)
BsoBI 3113 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3581 TGTACA T^GTACA
BspLU11I 1488 ACATGT A^CATGT
BsrGI 3581 TGTACA T^GTACA
BstEII 1684 GGTTACC G^GT[ACGT]ACC
BstPI 1684 GGTTACC G^GT[ACGT]ACC
BstUI 101 CGCG CG^CG
CelII 4461 GCTGAGC GC^T[ACGT]AGC
EagI 33 CGGCCG C^GGCCG
Eam1105I 3271 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 33 CGGCCG C^GGCCG
Eco47III 635 AGCGCT AGC^GCT
Eco52I 33 CGGCCG C^GGCCG
EcoO65I 1684 GGTTACC G^GT[ACGT]ACC
HincII 3526 GTCAAC GT[CT]^[GA]AC
HindII 3526 GTCAAC GT[CT]^[GA]AC
HindIII 862 AAGCTT A^AGCTT
KspI 100 CCGCGG CCGC^GG
MvnI 101 CGCG CG^CG
NcoI 808 CCATGG C^CATGG
PshBI 2583 ATTAAT AT^TAAT
SacII 100 CCGCGG CCGC^GG
SbfI 4595 CCTGCAGG CCTGCA^GG
ScaI 2428 AGTACT AGT^ACT
SexAI 4127 ACCAGGT A^CC[AT]GGT
SmaI 3113 CCCGGG CCC^GGG
SpeI 3548 ACTAGT A^CTAGT
SphI 1222 GCATGC GCATG^C
Sse8387I 4595 CCTGCAGG CCTGCA^GG
SspBI 3581 TGTACA T^GTACA
XbaI 1721 TCTAGA T^CTAGA
XmaCI 3113 CCCGGG C^CCGGG
XmaI 3113 CCCGGG C^CCGGG
XmnI 9 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 3795 GGTACC G^GTACC
4138 GGTACC
Asp718I 3795 GGTACC G^GTACC
4138 GGTACC
AviII 1023 TGCGCA TGC^GCA
2700 TGCGCA
BalI 891 TGGCCA TGG^CCA
3376 TGGCCA
BbeI 497 GGCGCC GGCGC^C
677 GGCGCC
BbsI 1309 GAAGAC GAAGAC(2/6)
3842 GAAGAC
BciVI 1462 GGATAC GTATCC(6/5)
1670 GGATAC
BpuAI 1309 GAAGAC GAAGAC(2/6)
3842 GAAGAC
BsrBI 604 GAGCGG CCGCTC(-3/-3)
1559 GAGCGG
DraIII 2964 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3241 CACTCTGTG
DrdI 75 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3230 GACACAGGGGTC
FauI 50 GCGGG CCCGC(4/6)
69 GCGGG
FspI 1023 TGCGCA TGC^GCA
2700 TGCGCA
KasI 497 GGCGCC G^GCGCC
677 GGCGCC
KpnI 3795 GGTACC GGTAC^C
4138 GGTACC
MluNI 891 TGGCCA TGG^CCA
3376 TGGCCA
MscI 891 TGGCCA TGG^CCA
3376 TGGCCA
NaeI 31 GCCGGC GCC^GGC
1218 GCCGGC
NarI 497 GGCGCC GG^CGCC
677 GGCGCC
NgoMIV 31 GCCGGC G^CCGGC
1218 GCCGGC
NheI 4076 GCTAGC G^CTAGC
4274 GCTAGC
PshAI 323 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2013 GACAGCTGTC
SfoI 497 GGCGCC GGC^GCC
677 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaII BamHI BanI BanII BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII