##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BsrFI BstBI CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
FspI HaeII KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2326 GGTACC G^GTACC
AcyI 2306 GACGCC G[GA]^CG[CT]C
AlwNI 206 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 666 CCTGAGG CC^T[ACGT]AGG
ApaI 1950 GGGCCC GGGCC^C
Asp718I 2326 GGTACC G^GTACC
AspHI 5201 GAGCAC G[AT]GC[AT]^C
AvaI 1807 CTCGGG C^[CT]CG[GA]G
BsaHI 2306 GACGCC G[GA]^CG[CT]C
BsaI 1900 GAGACC GGTCTC(1/5)
BsiHKAI 5201 GAGCAC G[AT]GC[AT]^C
BsoBI 1807 CTCGGG C^[CT]CG[GA]G
BspMI 4950 ACCTGC ACCTGC(4/8)
Bst1107I 2713 GTATAC GTA^TAC
BstAPI 276 GCAAACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2207 GGTAACC G^GT[ACGT]ACC
BstPI 2207 GGTAACC G^GT[ACGT]ACC
BstZ17I 2713 GTATAC GTA^TAC
Bsu36I 666 CCTGAGG CC^T[ACGT]AGG
DrdI 1042 GACACAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 666 CCTGAGG CC^T[ACGT]AGG
EcoNI 1320 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2207 GGTAACC G^GT[ACGT]ACC
EcoRV 543 GATATC GAT^ATC
Hin1I 2306 GACGCC G[GA]^CG[CT]C
HindIII 4228 AAGCTT A^AGCTT
HpaI 3417 GTTAAC GTT^AAC
KpnI 2326 GGTACC GGTAC^C
NcoI 3150 CCATGG C^CATGG
PvuI 1183 CGATCG CGAT^CG
PvuII 1716 CAGCTG CAG^CTG
StuI 4029 AGGCCT AGG^CCT
XcmI 2926 CCAAAACGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsaBI 1478 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1938 GATAATCATC
BseRI 2054 GAGGAG GAGGAG(10/8)
2076 GAGGAG
BsiEI 710 CGGTCG CG[GA][CT]^CG
1183 CGATCG
BsrBI 2514 GAGCGG CCGCTC(-3/-3)
3694 GAGCGG
BsrDI 5384 CATTGC GCAATG(2/0)
1689 GCAATG
BssSI 2505 CACGAG CACGAG(-5/-1)
2967 CTCGTG
BstXI 3059 CCAGAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4011 CCAGTTCATTGG
DraIII 2169 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3009 CACTGAGTG
DsaI 1344 CCACGG C^C[GA][CT]GG
3150 CCATGG
HgaI 2306 GACGC GACGC(5/10)
208 GCGTC
MaeII 2757 ACGT A^CGT
2931 ACGT
MamI 1478 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1938 GATAATCATC
NdeI 1282 CATATG CA^TATG
4924 CATATG
SapI 719 GAAGAGC GCTCTTC(1/4)
5334 GAAGAGC
SwaI 3139 ATTTAAAT ATTT^AAAT
4254 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI ApoI
Asp700I AvaII BalI BanI BanII
BbsI BbvI BcgI BclI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI