##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI Asp718I AspEI AvrII
BanI BbeI BclI BlnI BlpI
Bpu1102I BsiEI BsiWI BspDI BspLU11I
BsrDI BsrFI BssHII CelII Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV FbaI
FseI HindIII HpaI KasI KpnI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NspI
PacI PinAI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 515 GTATAC GT^[AC][GT]AC
AccIII 3555 TCCGGA T^CCGGA
AflII 2716 CTTAAG C^TTAAG
Alw44I 3567 GTGCAC G^TGCAC
Aor51HI 1761 AGCGCT AGC^GCT
ApaLI 3567 GTGCAC G^TGCAC
AspI 1889 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 853 TGCGCA TGC^GCA
BamHI 3299 GGATCC G^GATCC
BbrPI 1669 CACGTG CAC^GTG
BbvCI 4079 GCTGAGG CCTCAGC(-5/-2)
BciVI 3552 GTATCC GTATCC(6/5)
BfrI 2716 CTTAAG C^TTAAG
BglI 305 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1669 CACGTG [CT]AC^GT[GA]
BseAI 3555 TCCGGA T^CCGGA
BspEI 3555 TCCGGA T^CCGGA
BsrBI 112 CCGCTC CCGCTC(-3/-3)
Bst1107I 515 GTATAC GTA^TAC
BstBI 2838 TTCGAA TT^CGAA
BstZ17I 515 GTATAC GTA^TAC
DrdI 4353 GACATACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1761 AGCGCT AGC^GCT
FspI 853 TGCGCA TGC^GCA
KspI 69 CCGCGG CCGC^GG
MroI 3555 TCCGGA T^CCGGA
NdeI 1614 CATATG CA^TATG
NspV 2838 TTCGAA TT^CGAA
PaeR7I 4317 CTCGAG C^TCGAG
PflFI 1889 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1669 CACGTG CAC^GTG
PmeI 2001 GTTTAAAC GTTT^AAAC
PmlI 1669 CACGTG CAC^GTG
PshAI 2030 GACGTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2244 CTGCAG CTGCA^G
PvuII 1194 CAGCTG CAG^CTG
SacII 69 CCGCGG CCGC^GG
ScaI 1500 AGTACT AGT^ACT
SfuI 2838 TTCGAA TT^CGAA
SmaI 94 CCCGGG CCC^GGG
Tth111I 1889 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4317 CTCGAG C^TCGAG
XmaCI 94 CCCGGG C^CCGGG
XmaI 94 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 106 GACGCC G[GA]^CG[CT]C
1439 GGCGTC
AflIII 1221 ACACGT A^C[GA][CT]GT
1668 ACACGT
AseI 3646 ATTAAT AT^TAAT
4604 ATTAAT
AsnI 3646 ATTAAT AT^TAAT
4604 ATTAAT
Asp700I 3191 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3950 GAAAATCTTC
BalI 1950 TGGCCA TGG^CCA
2399 TGGCCA
BcgI 3400 CGAGGACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3288 GCAAACTATTCG
BglII 826 AGATCT A^GATCT
4240 AGATCT
BsaHI 106 GACGCC G[GA]^CG[CT]C
1439 GGCGTC
BsaWI 2439 ACCGGA [AT]^CCGG[AT]
3555 TCCGGA
BspHI 1785 TCATGA T^CATGA
4264 TCATGA
BstAPI 2917 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3569 GCACGGTCTGC
BstXI 1115 CCACCGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1664 CCAGACACGTGG
DraI 2002 TTTAAA TTT^AAA
4586 TTTAAA
DraIII 1669 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2637 CACTTAGTG
EaeI 1950 TGGCCA [CT]^GGCC[GA]
2399 TGGCCA
EcoT22I 3212 ATGCAT ATGCA^T
3839 ATGCAT
Hin1I 106 GACGCC G[GA]^CG[CT]C
1439 GGCGTC
HincII 1214 GTCAAC GT[CT]^[GA]AC
3124 GTTGAC
HindII 1214 GTCAAC GT[CT]^[GA]AC
3124 GTTGAC
MluNI 1950 TGGCCA TGG^CCA
2399 TGGCCA
MscI 1950 TGGCCA TGG^CCA
2399 TGGCCA
MspA1I 69 CCGCGG C[AC]G^C[GT]G
1194 CAGCTG
NcoI 270 CCATGG C^CATGG
1165 CCATGG
NsiI 3212 ATGCAT ATGCA^T
3839 ATGCAT
PshBI 3646 ATTAAT AT^TAAT
4604 ATTAAT
RcaI 1785 TCATGA T^CATGA
4264 TCATGA
StuI 2180 AGGCCT AGG^CCT
2787 AGGCCT
XmnI 3191 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3950 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII