##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I ApaLI AscI Asp700I AspI
AviII AvrII BbeI BciVI BlnI
BsaHI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII EcoRV FseI FspI
Hin1I HindIII HpaI KasI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 796 GGTACC G^GTACC
AccII 11 CGCG CG^CG
AflII 1550 CTTAAG C^TTAAG
AhdI 225 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2006 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2916 AGCGCT AGC^GCT
AseI 4197 ATTAAT AT^TAAT
AsnI 4197 ATTAAT AT^TAAT
Asp718I 796 GGTACC G^GTACC
AspEI 225 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3045 GGATCC G^GATCC
BbrPI 4097 CACGTG CAC^GTG
BcgI 3901 GCAGAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1550 CTTAAG C^TTAAG
BsaBI 2638 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4136 GGTCTC GGTCTC(1/5)
BsaWI 2538 ACCGGA [AT]^CCGG[AT]
BspLU11I 2867 ACATGT A^CATGT
BssSI 2329 CACGAG CACGAG(-5/-1)
BstEII 2317 GGTGACC G^GT[ACGT]ACC
BstPI 2317 GGTGACC G^GT[ACGT]ACC
BstUI 11 CGCG CG^CG
BstXI 2469 CCAACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2006 CCTCAGG CC^T[ACGT]AGG
DraI 4275 TTTAAA TTT^AAA
DrdI 2087 GACCAGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 7 CGGCCG C^GGCCG
Eam1105I 225 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 7 CGGCCG C^GGCCG
Eco47III 2916 AGCGCT AGC^GCT
Eco52I 7 CGGCCG C^GGCCG
Eco81I 2006 CCTCAGG CC^T[ACGT]AGG
EcoNI 2473 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2317 GGTGACC G^GT[ACGT]ACC
EcoRI 1343 GAATTC G^AATTC
EcoT22I 3212 ATGCAT ATGCA^T
HaeII 2916 AGCGCT [GA]GCGC^[CT]
KpnI 796 GGTACC GGTAC^C
KspI 10 CCGCGG CCGC^GG
MamI 2638 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 11 CGCG CG^CG
NaeI 155 GCCGGC GCC^GGC
NdeI 4029 CATATG CA^TATG
NgoMIV 155 GCCGGC G^CCGGC
NsiI 3212 ATGCAT ATGCA^T
PflMI 2779 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4097 CACGTG CAC^GTG
PmlI 4097 CACGTG CAC^GTG
PshBI 4197 ATTAAT AT^TAAT
SacI 2245 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SalI 1649 GTCGAC G^TCGAC
SexAI 2472 ACCTGGT A^CC[AT]GGT
SmaI 1618 CCCGGG CCC^GGG
Van91I 2779 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1618 CCCGGG C^CCGGG
XmaI 1618 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1649 GTCGAC GT^[AC][GT]AC
3745 GTCTAC
AcsI 1343 GAATTC [GA]^AATT[CT]
2947 GAATTT
ApaI 1556 GGGCCC GGGCC^C
3713 GGGCCC
ApoI 1343 GAATTC [GA]^AATT[CT]
2947 GAATTT
BanI 158 GGCACC G^G[CT][GA]CC
796 GGTACC
BbsI 385 GAAGAC GAAGAC(2/6)
1583 GAAGAC
BglI 464 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3307 GCCAAACGGGC
BpuAI 385 GAAGAC GAAGAC(2/6)
1583 GAAGAC
BsaAI 1388 TACGTG [CT]AC^GT[GA]
4097 CACGTG
BsiEI 7 CGGCCG CG[GA][CT]^CG
13 CGGTCG
BsmBI 2098 CGTCTC CGTCTC(1/5)
2405 GAGACG
CfoI 2917 GCGC GCG^C
3339 GCGC
FauI 245 CCCGC CCCGC(4/6)
3958 GCGGG
HgaI 1158 GCGTC GACGC(5/10)
3743 GCGTC
HhaI 2917 GCGC GCG^C
3339 GCGC
HinP1I 2917 GCGC G^CGC
3339 GCGC
HincII 349 GTCAAC GT[CT]^[GA]AC
1649 GTCGAC
HindII 349 GTCAAC GT[CT]^[GA]AC
1649 GTCGAC
StuI 1089 AGGCCT AGG^CCT
1333 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanII BbvCI BbvI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XhoII