################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccIII AclI AcyI AgeI Alw44I ApaLI AscI Asp700I AspI AviII AvrII BbeI BciVI BlnI BsaHI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraIII EcoRV FseI FspI Hin1I HindIII HpaI KasI MfeI MluI MroI MunI NarI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 796 GGTACC G^GTACC AccII 11 CGCG CG^CG AflII 1550 CTTAAG C^TTAAG AhdI 225 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2006 CCTCAGG CC^T[ACGT]AGG Aor51HI 2916 AGCGCT AGC^GCT AseI 4197 ATTAAT AT^TAAT AsnI 4197 ATTAAT AT^TAAT Asp718I 796 GGTACC G^GTACC AspEI 225 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3045 GGATCC G^GATCC BbrPI 4097 CACGTG CAC^GTG BcgI 3901 GCAGAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1550 CTTAAG C^TTAAG BsaBI 2638 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4136 GGTCTC GGTCTC(1/5) BsaWI 2538 ACCGGA [AT]^CCGG[AT] BspLU11I 2867 ACATGT A^CATGT BssSI 2329 CACGAG CACGAG(-5/-1) BstEII 2317 GGTGACC G^GT[ACGT]ACC BstPI 2317 GGTGACC G^GT[ACGT]ACC BstUI 11 CGCG CG^CG BstXI 2469 CCAACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2006 CCTCAGG CC^T[ACGT]AGG DraI 4275 TTTAAA TTT^AAA DrdI 2087 GACCAGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 7 CGGCCG C^GGCCG Eam1105I 225 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 7 CGGCCG C^GGCCG Eco47III 2916 AGCGCT AGC^GCT Eco52I 7 CGGCCG C^GGCCG Eco81I 2006 CCTCAGG CC^T[ACGT]AGG EcoNI 2473 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2317 GGTGACC G^GT[ACGT]ACC EcoRI 1343 GAATTC G^AATTC EcoT22I 3212 ATGCAT ATGCA^T HaeII 2916 AGCGCT [GA]GCGC^[CT] KpnI 796 GGTACC GGTAC^C KspI 10 CCGCGG CCGC^GG MamI 2638 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 11 CGCG CG^CG NaeI 155 GCCGGC GCC^GGC NdeI 4029 CATATG CA^TATG NgoMIV 155 GCCGGC G^CCGGC NsiI 3212 ATGCAT ATGCA^T PflMI 2779 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4097 CACGTG CAC^GTG PmlI 4097 CACGTG CAC^GTG PshBI 4197 ATTAAT AT^TAAT SacI 2245 GAGCTC GAGCT^C SacII 10 CCGCGG CCGC^GG SalI 1649 GTCGAC G^TCGAC SexAI 2472 ACCTGGT A^CC[AT]GGT SmaI 1618 CCCGGG CCC^GGG Van91I 2779 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1618 CCCGGG C^CCGGG XmaI 1618 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 1649 GTCGAC GT^[AC][GT]AC 3745 GTCTAC AcsI 1343 GAATTC [GA]^AATT[CT] 2947 GAATTT ApaI 1556 GGGCCC GGGCC^C 3713 GGGCCC ApoI 1343 GAATTC [GA]^AATT[CT] 2947 GAATTT BanI 158 GGCACC G^G[CT][GA]CC 796 GGTACC BbsI 385 GAAGAC GAAGAC(2/6) 1583 GAAGAC BglI 464 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3307 GCCAAACGGGC BpuAI 385 GAAGAC GAAGAC(2/6) 1583 GAAGAC BsaAI 1388 TACGTG [CT]AC^GT[GA] 4097 CACGTG BsiEI 7 CGGCCG CG[GA][CT]^CG 13 CGGTCG BsmBI 2098 CGTCTC CGTCTC(1/5) 2405 GAGACG CfoI 2917 GCGC GCG^C 3339 GCGC FauI 245 CCCGC CCCGC(4/6) 3958 GCGGG HgaI 1158 GCGTC GACGC(5/10) 3743 GCGTC HhaI 2917 GCGC GCG^C 3339 GCGC HinP1I 2917 GCGC G^CGC 3339 GCGC HincII 349 GTCAAC GT[CT]^[GA]AC 1649 GTCGAC HindII 349 GTCAAC GT[CT]^[GA]AC 1649 GTCGAC StuI 1089 AGGCCT AGG^CCT 1333 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanII BbvCI BbvI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII