##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BbeI
BbrPI BbvCI BlnI BsaAI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FseI
FspI HaeII KasI KspI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1263 GGCGTC G[GA]^CG[CT]C
AflII 2439 CTTAAG C^TTAAG
BcgI 1292 CGAGCTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3971 GGATAC GTATCC(6/5)
BclI 167 TGATCA T^GATCA
BfrI 2439 CTTAAG C^TTAAG
BsaBI 4297 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1263 GGCGTC G[GA]^CG[CT]C
Bsp1407I 3336 TGTACA T^GTACA
BspLU11I 4352 ACATGT A^CATGT
BsrFI 637 ACCGGC [GA]^CCGG[CT]
BsrGI 3336 TGTACA T^GTACA
Cfr10I 637 ACCGGC [GA]^CCGG[CT]
DraIII 1002 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1257 GACAGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1904 CCATGG C^C[GA][CT]GG
EcoRI 97 GAATTC G^AATTC
EcoRV 558 GATATC GAT^ATC
FbaI 167 TGATCA T^GATCA
Hin1I 1263 GGCGTC G[GA]^CG[CT]C
HpaI 4173 GTTAAC GTT^AAC
MaeII 1152 ACGT A^CGT
MamI 4297 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1904 CCATGG C^CATGG
NheI 2750 GCTAGC G^CTAGC
SacI 3639 GAGCTC GAGCT^C
SexAI 1084 ACCAGGT A^CC[AT]GGT
SspBI 3336 TGTACA T^GTACA
StuI 4141 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1683 GGTACC G^GTACC
2290 GGTACC
AccIII 1457 TCCGGA T^CCGGA
2894 TCCGGA
AflIII 1150 ACACGT A^C[GA][CT]GT
4352 ACATGT
AocI 2526 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
ApaI 2407 GGGCCC GGGCC^C
3708 GGGCCC
Asp718I 1683 GGTACC G^GTACC
2290 GGTACC
AvaI 664 CCCGGG C^[CT]CG[GA]G
1105 CCCGGG
BglII 3748 AGATCT A^GATCT
4296 AGATCT
BsaI 2735 GAGACC GGTCTC(1/5)
4272 GGTCTC
BseAI 1457 TCCGGA T^CCGGA
2894 TCCGGA
BsgI 3352 GTGCAG GTGCAG(16/14)
3598 GTGCAG
BsoBI 664 CCCGGG C^[CT]CG[GA]G
1105 CCCGGG
BspEI 1457 TCCGGA T^CCGGA
2894 TCCGGA
Bsu36I 2526 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
CfoI 954 GCGC GCG^C
2429 GCGC
EarI 1848 GAAGAG CTCTTC(1/4)
2837 GAAGAG
Eco81I 2526 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
FauI 33 GCGGG CCCGC(4/6)
1637 GCGGG
HhaI 954 GCGC GCG^C
2429 GCGC
HinP1I 954 GCGC G^CGC
2429 GCGC
HincII 2886 GTCAAC GT[CT]^[GA]AC
4173 GTTAAC
HindII 2886 GTCAAC GT[CT]^[GA]AC
4173 GTTAAC
HindIII 1416 AAGCTT A^AGCTT
4112 AAGCTT
KpnI 1683 GGTACC GGTAC^C
2290 GGTACC
Ksp632I 1848 GAAGAG CTCTTC(1/4)
2837 GAAGAG
MroI 1457 TCCGGA T^CCGGA
2894 TCCGGA
PstI 3000 CTGCAG CTGCA^G
4267 CTGCAG
SmaI 664 CCCGGG CCC^GGG
1105 CCCGGG
XbaI 582 TCTAGA T^CTAGA
4146 TCTAGA
XmaCI 664 CCCGGG C^CCGGG
1105 CCCGGG
XmaI 664 CCCGGG C^CCGGG
1105 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII