################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BbrPI BbvCI BlnI BsaAI BsiEI BsiWI BsmBI BsmI BspDI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcyI 1263 GGCGTC G[GA]^CG[CT]C AflII 2439 CTTAAG C^TTAAG BcgI 1292 CGAGCTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3971 GGATAC GTATCC(6/5) BclI 167 TGATCA T^GATCA BfrI 2439 CTTAAG C^TTAAG BsaBI 4297 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1263 GGCGTC G[GA]^CG[CT]C Bsp1407I 3336 TGTACA T^GTACA BspLU11I 4352 ACATGT A^CATGT BsrFI 637 ACCGGC [GA]^CCGG[CT] BsrGI 3336 TGTACA T^GTACA Cfr10I 637 ACCGGC [GA]^CCGG[CT] DraIII 1002 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1257 GACAGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1904 CCATGG C^C[GA][CT]GG EcoRI 97 GAATTC G^AATTC EcoRV 558 GATATC GAT^ATC FbaI 167 TGATCA T^GATCA Hin1I 1263 GGCGTC G[GA]^CG[CT]C HpaI 4173 GTTAAC GTT^AAC MaeII 1152 ACGT A^CGT MamI 4297 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1904 CCATGG C^CATGG NheI 2750 GCTAGC G^CTAGC SacI 3639 GAGCTC GAGCT^C SexAI 1084 ACCAGGT A^CC[AT]GGT SspBI 3336 TGTACA T^GTACA StuI 4141 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 1683 GGTACC G^GTACC 2290 GGTACC AccIII 1457 TCCGGA T^CCGGA 2894 TCCGGA AflIII 1150 ACACGT A^C[GA][CT]GT 4352 ACATGT AocI 2526 CCTCAGG CC^T[ACGT]AGG 2642 CCTGAGG ApaI 2407 GGGCCC GGGCC^C 3708 GGGCCC Asp718I 1683 GGTACC G^GTACC 2290 GGTACC AvaI 664 CCCGGG C^[CT]CG[GA]G 1105 CCCGGG BglII 3748 AGATCT A^GATCT 4296 AGATCT BsaI 2735 GAGACC GGTCTC(1/5) 4272 GGTCTC BseAI 1457 TCCGGA T^CCGGA 2894 TCCGGA BsgI 3352 GTGCAG GTGCAG(16/14) 3598 GTGCAG BsoBI 664 CCCGGG C^[CT]CG[GA]G 1105 CCCGGG BspEI 1457 TCCGGA T^CCGGA 2894 TCCGGA Bsu36I 2526 CCTCAGG CC^T[ACGT]AGG 2642 CCTGAGG CfoI 954 GCGC GCG^C 2429 GCGC EarI 1848 GAAGAG CTCTTC(1/4) 2837 GAAGAG Eco81I 2526 CCTCAGG CC^T[ACGT]AGG 2642 CCTGAGG FauI 33 GCGGG CCCGC(4/6) 1637 GCGGG HhaI 954 GCGC GCG^C 2429 GCGC HinP1I 954 GCGC G^CGC 2429 GCGC HincII 2886 GTCAAC GT[CT]^[GA]AC 4173 GTTAAC HindII 2886 GTCAAC GT[CT]^[GA]AC 4173 GTTAAC HindIII 1416 AAGCTT A^AGCTT 4112 AAGCTT KpnI 1683 GGTACC GGTAC^C 2290 GGTACC Ksp632I 1848 GAAGAG CTCTTC(1/4) 2837 GAAGAG MroI 1457 TCCGGA T^CCGGA 2894 TCCGGA PstI 3000 CTGCAG CTGCA^G 4267 CTGCAG SmaI 664 CCCGGG CCC^GGG 1105 CCCGGG XbaI 582 TCTAGA T^CTAGA 4146 TCTAGA XmaCI 664 CCCGGG C^CCGGG 1105 CCCGGG XmaI 664 CCCGGG C^CCGGG 1105 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII