################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccIII AclI AcyI AflII AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BstBI CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I FauI FseI FspI Hin1I HindIII HpaI KasI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 2063 GGTACC G^GTACC AccI 1398 GTATAC GT^[AC][GT]AC AflIII 4099 ACATGT A^C[GA][CT]GT AgeI 2066 ACCGGT A^CCGGT Alw44I 2163 GTGCAC G^TGCAC ApaLI 2163 GTGCAC G^TGCAC Asp718I 2063 GGTACC G^GTACC BamHI 1163 GGATCC G^GATCC BclI 4483 TGATCA T^GATCA BsaAI 4059 CACGTA [CT]AC^GT[GA] BsaWI 2066 ACCGGT [AT]^CCGG[AT] BsmBI 3789 GAGACG CGTCTC(1/5) BspLU11I 4099 ACATGT A^CATGT BsrBI 13 GAGCGG CCGCTC(-3/-3) BsrFI 2066 ACCGGT [GA]^CCGG[CT] Bst1107I 1398 GTATAC GTA^TAC BstAPI 2302 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 4637 CCAAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1398 GTATAC GTA^TAC Cfr10I 2066 ACCGGT [GA]^CCGG[CT] EarI 3821 GAAGAG CTCTTC(1/4) EcoRV 4454 GATATC GAT^ATC EcoT22I 4030 ATGCAT ATGCA^T FbaI 4483 TGATCA T^GATCA HgaI 939 GACGC GACGC(5/10) HincII 4783 GTCAAC GT[CT]^[GA]AC HindII 4783 GTCAAC GT[CT]^[GA]AC KpnI 2063 GGTACC GGTAC^C Ksp632I 3821 GAAGAG CTCTTC(1/4) MfeI 3516 CAATTG C^AATTG MunI 3516 CAATTG C^AATTG NdeI 606 CATATG CA^TATG NsiI 4030 ATGCAT ATGCA^T PflMI 569 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2066 ACCGGT A^CCGGT SfiI 3278 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 1063 AGGCCT AGG^CCT Van91I 569 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 1466 CGCG CG^CG 2717 CGCG BciVI 722 GGATAC GTATCC(6/5) 1731 GGATAC BglI 885 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3279 GCCCACCTGGC BsaI 3915 GAGACC GGTCTC(1/5) 524 GGTCTC BstEII 497 GGTGACC G^GT[ACGT]ACC 3503 GGTCACC BstPI 497 GGTGACC G^GT[ACGT]ACC 3503 GGTCACC BstUI 1466 CGCG CG^CG 2717 CGCG CfoI 2595 GCGC GCG^C 3261 GCGC EcoNI 2237 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3968 CCTAACCAAGG EcoO65I 497 GGTGACC G^GT[ACGT]ACC 3503 GGTCACC EcoRI 1497 GAATTC G^AATTC 4322 GAATTC HaeII 2594 GGCGCT [GA]GCGC^[CT] 3260 GGCGCT HhaI 2595 GCGC GCG^C 3261 GCGC HinP1I 2595 GCGC G^CGC 3261 GCGC MseI 3849 TTAA T^TAA 4586 TTAA MvnI 1466 CGCG CG^CG 2717 CGCG NcoI 439 CCATGG C^CATGG 1023 CCATGG SmaI 1286 CCCGGG CCC^GGG 2563 CCCGGG SphI 894 GCATGC GCATG^C 3018 GCATGC Tru9I 3849 TTAA T^TAA 4586 TTAA XbaI 257 TCTAGA T^CTAGA 4363 TCTAGA XmaCI 1286 CCCGGG C^CCGGG 2563 CCCGGG XmaI 1286 CCCGGG C^CCGGG 2563 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SmlI Sse8387I StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII