##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BstBI CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI Hin1I HindIII
HpaI KasI KspI MamI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2063 GGTACC G^GTACC
AccI 1398 GTATAC GT^[AC][GT]AC
AflIII 4099 ACATGT A^C[GA][CT]GT
AgeI 2066 ACCGGT A^CCGGT
Alw44I 2163 GTGCAC G^TGCAC
ApaLI 2163 GTGCAC G^TGCAC
Asp718I 2063 GGTACC G^GTACC
BamHI 1163 GGATCC G^GATCC
BclI 4483 TGATCA T^GATCA
BsaAI 4059 CACGTA [CT]AC^GT[GA]
BsaWI 2066 ACCGGT [AT]^CCGG[AT]
BsmBI 3789 GAGACG CGTCTC(1/5)
BspLU11I 4099 ACATGT A^CATGT
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrFI 2066 ACCGGT [GA]^CCGG[CT]
Bst1107I 1398 GTATAC GTA^TAC
BstAPI 2302 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4637 CCAAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1398 GTATAC GTA^TAC
Cfr10I 2066 ACCGGT [GA]^CCGG[CT]
EarI 3821 GAAGAG CTCTTC(1/4)
EcoRV 4454 GATATC GAT^ATC
EcoT22I 4030 ATGCAT ATGCA^T
FbaI 4483 TGATCA T^GATCA
HgaI 939 GACGC GACGC(5/10)
HincII 4783 GTCAAC GT[CT]^[GA]AC
HindII 4783 GTCAAC GT[CT]^[GA]AC
KpnI 2063 GGTACC GGTAC^C
Ksp632I 3821 GAAGAG CTCTTC(1/4)
MfeI 3516 CAATTG C^AATTG
MunI 3516 CAATTG C^AATTG
NdeI 606 CATATG CA^TATG
NsiI 4030 ATGCAT ATGCA^T
PflMI 569 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2066 ACCGGT A^CCGGT
SfiI 3278 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1063 AGGCCT AGG^CCT
Van91I 569 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1466 CGCG CG^CG
2717 CGCG
BciVI 722 GGATAC GTATCC(6/5)
1731 GGATAC
BglI 885 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3279 GCCCACCTGGC
BsaI 3915 GAGACC GGTCTC(1/5)
524 GGTCTC
BstEII 497 GGTGACC G^GT[ACGT]ACC
3503 GGTCACC
BstPI 497 GGTGACC G^GT[ACGT]ACC
3503 GGTCACC
BstUI 1466 CGCG CG^CG
2717 CGCG
CfoI 2595 GCGC GCG^C
3261 GCGC
EcoNI 2237 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3968 CCTAACCAAGG
EcoO65I 497 GGTGACC G^GT[ACGT]ACC
3503 GGTCACC
EcoRI 1497 GAATTC G^AATTC
4322 GAATTC
HaeII 2594 GGCGCT [GA]GCGC^[CT]
3260 GGCGCT
HhaI 2595 GCGC GCG^C
3261 GCGC
HinP1I 2595 GCGC G^CGC
3261 GCGC
MseI 3849 TTAA T^TAA
4586 TTAA
MvnI 1466 CGCG CG^CG
2717 CGCG
NcoI 439 CCATGG C^CATGG
1023 CCATGG
SmaI 1286 CCCGGG CCC^GGG
2563 CCCGGG
SphI 894 GCATGC GCATG^C
3018 GCATGC
Tru9I 3849 TTAA T^TAA
4586 TTAA
XbaI 257 TCTAGA T^CTAGA
4363 TCTAGA
XmaCI 1286 CCCGGG C^CCGGG
2563 CCCGGG
XmaI 1286 CCCGGG C^CCGGG
2563 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SexAI SfaNI
SfcI SmlI Sse8387I StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XhoII