##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        BbeI        BbrPI       BciVI       BclI        
BfrI        BsaAI       BseAI       BsiWI       BsmBI       
BspDI       BspEI       BspHI       BssSI       Bst1107I    
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        KasI        MfeI        
MluI        MroI        MunI        NarI        NcoI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        1012  GACGTC               GACGT^C
Acc65I       1532  GGTACC               G^GTACC
AcyI         1012  GACGTC               G[GA]^CG[CT]C
AhdI         1513  GACTGTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       107  AGCGCT               AGC^GCT
Asp700I      2693  GAATAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1532  GGTACC               G^GTACC
AspEI        1513  GACTGTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         710  TGCGCA               TGC^GCA
AvrII        2631  CCTAGG               C^CTAGG
BanI         1532  GGTACC               G^G[CT][GA]CC
BbvCI        1451  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1359  CGAGGGCAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1313  GCCGAGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1056  AGATCT               A^GATCT
BlnI         2631  CCTAGG               C^CTAGG
BsaBI        1230  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1012  GACGTC               G[GA]^CG[CT]C
BsmI         1860  GCATTC               GAATGC(1/-1)
BsrBI         460  CCGCTC               CCGCTC(-3/-3)
BssHII        407  GCGCGC               G^CGCGC
BstBI        2983  TTCGAA               TT^CGAA
DsaI          402  CCGCGG               C^C[GA][CT]GG
EagI          399  CGGCCG               C^GGCCG
Eam1105I     1513  GACTGTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         399  CGGCCG               C^GGCCG
Eco47III      107  AGCGCT               AGC^GCT
Eco52I        399  CGGCCG               C^GGCCG
FspI          710  TGCGCA               TGC^GCA
Hin1I        1012  GACGTC               G[GA]^CG[CT]C
HindIII      3615  AAGCTT               A^AGCTT
HpaI         1606  GTTAAC               GTT^AAC
KpnI         1532  GGTACC               GGTAC^C
KspI          402  CCGCGG               CCGC^GG
MamI         1230  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI          398  GCGGCCGC             GC^GGCCGC
NspV         2983  TTCGAA               TT^CGAA
SacII         402  CCGCGG               CCGC^GG
SapI          720  GAAGAGC              GCTCTTC(1/4)
SexAI        3452  ACCAGGT              A^CC[AT]GGT
SfuI         2983  TTCGAA               TT^CGAA
SmaI          872  CCCGGG               CCC^GGG
SmlI         3254  CTTGAG               C^T[CT][GA]AG
SphI         2138  GCATGC               GCATG^C
StuI         1466  AGGCCT               AGG^CCT
XbaI         3752  TCTAGA               T^CTAGA
XmaCI         872  CCCGGG               C^CCGGG
XmaI          872  CCCGGG               C^CCGGG
XmnI         2693  GAATAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI         1251  GAATTT               [GA]^AATT[CT]
             3140  AAATTC              
ApoI         1251  GAATTT               [GA]^AATT[CT]
             3140  AAATTC              
BalI         1481  TGGCCA               TGG^CCA
             3945  TGGCCA              
BlpI         2710  GCTTAGC              GC^T[ACGT]AGC
             3882  GCTCAGC             
Bpu1102I     2710  GCTTAGC              GC^T[ACGT]AGC
             3882  GCTCAGC             
BsiEI         399  CGGCCG               CG[GA][CT]^CG
             2071  CGACCG              
BspLU11I     3297  ACATGT               A^CATGT
             4022  ACATGT              
BspMI         653  ACCTGC               ACCTGC(4/8)
             3022  ACCTGC              
CelII        2710  GCTTAGC              GC^T[ACGT]AGC
             3882  GCTCAGC             
DraIII       2451  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3451  CACCAGGTG           
HaeII         107  AGCGCT               [GA]GCGC^[CT]
              274  AGCGCC              
HgaI          158  GCGTC                GACGC(5/10)
              725  GCGTC               
HincII       1606  GTTAAC               GT[CT]^[GA]AC
             2365  GTCAAC              
HindII       1606  GTTAAC               GT[CT]^[GA]AC
             2365  GTCAAC              
MluNI        1481  TGGCCA               TGG^CCA
             3945  TGGCCA              
MscI         1481  TGGCCA               TGG^CCA
             3945  TGGCCA              
NdeI         2616  CATATG               CA^TATG
             2717  CATATG              
PflMI        2754  CCACTAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3467  CCAAGTCCTGG         
PpuMI         539  AGGACCC              [GA]G^G[AT]CC[CT]
             2601  AGGTCCC             
PshAI        1075  GACTGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2638  GACCACAGTC          
Van91I       2754  CCACTAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3467  CCAAGTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApaI        AspHI       AspI        
AvaI        AvaII       BamHI       BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BsrDI       
BsrFI       BsrGI       BsrI        BssKI       BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NheI        NlaIII      NlaIV       NspI        PflFI       
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XcmI        XhoII