##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I AocI ApaLI AscI AseI
AsnI BbeI BbrPI BciVI BclI
BfrI BsaAI BseAI BsiWI BsmBI
BspDI BspEI BspHI BssSI Bst1107I
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco81I EcoRI EcoRV
EcoT22I FbaI FseI KasI MfeI
MluI MroI MunI NarI NcoI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1012 GACGTC GACGT^C
Acc65I 1532 GGTACC G^GTACC
AcyI 1012 GACGTC G[GA]^CG[CT]C
AhdI 1513 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 107 AGCGCT AGC^GCT
Asp700I 2693 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1532 GGTACC G^GTACC
AspEI 1513 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 710 TGCGCA TGC^GCA
AvrII 2631 CCTAGG C^CTAGG
BanI 1532 GGTACC G^G[CT][GA]CC
BbvCI 1451 CCTCAGC CCTCAGC(-5/-2)
BcgI 1359 CGAGGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1313 GCCGAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1056 AGATCT A^GATCT
BlnI 2631 CCTAGG C^CTAGG
BsaBI 1230 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1012 GACGTC G[GA]^CG[CT]C
BsmI 1860 GCATTC GAATGC(1/-1)
BsrBI 460 CCGCTC CCGCTC(-3/-3)
BssHII 407 GCGCGC G^CGCGC
BstBI 2983 TTCGAA TT^CGAA
DsaI 402 CCGCGG C^C[GA][CT]GG
EagI 399 CGGCCG C^GGCCG
Eam1105I 1513 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 399 CGGCCG C^GGCCG
Eco47III 107 AGCGCT AGC^GCT
Eco52I 399 CGGCCG C^GGCCG
FspI 710 TGCGCA TGC^GCA
Hin1I 1012 GACGTC G[GA]^CG[CT]C
HindIII 3615 AAGCTT A^AGCTT
HpaI 1606 GTTAAC GTT^AAC
KpnI 1532 GGTACC GGTAC^C
KspI 402 CCGCGG CCGC^GG
MamI 1230 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 398 GCGGCCGC GC^GGCCGC
NspV 2983 TTCGAA TT^CGAA
SacII 402 CCGCGG CCGC^GG
SapI 720 GAAGAGC GCTCTTC(1/4)
SexAI 3452 ACCAGGT A^CC[AT]GGT
SfuI 2983 TTCGAA TT^CGAA
SmaI 872 CCCGGG CCC^GGG
SmlI 3254 CTTGAG C^T[CT][GA]AG
SphI 2138 GCATGC GCATG^C
StuI 1466 AGGCCT AGG^CCT
XbaI 3752 TCTAGA T^CTAGA
XmaCI 872 CCCGGG C^CCGGG
XmaI 872 CCCGGG C^CCGGG
XmnI 2693 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1251 GAATTT [GA]^AATT[CT]
3140 AAATTC
ApoI 1251 GAATTT [GA]^AATT[CT]
3140 AAATTC
BalI 1481 TGGCCA TGG^CCA
3945 TGGCCA
BlpI 2710 GCTTAGC GC^T[ACGT]AGC
3882 GCTCAGC
Bpu1102I 2710 GCTTAGC GC^T[ACGT]AGC
3882 GCTCAGC
BsiEI 399 CGGCCG CG[GA][CT]^CG
2071 CGACCG
BspLU11I 3297 ACATGT A^CATGT
4022 ACATGT
BspMI 653 ACCTGC ACCTGC(4/8)
3022 ACCTGC
CelII 2710 GCTTAGC GC^T[ACGT]AGC
3882 GCTCAGC
DraIII 2451 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3451 CACCAGGTG
HaeII 107 AGCGCT [GA]GCGC^[CT]
274 AGCGCC
HgaI 158 GCGTC GACGC(5/10)
725 GCGTC
HincII 1606 GTTAAC GT[CT]^[GA]AC
2365 GTCAAC
HindII 1606 GTTAAC GT[CT]^[GA]AC
2365 GTCAAC
MluNI 1481 TGGCCA TGG^CCA
3945 TGGCCA
MscI 1481 TGGCCA TGG^CCA
3945 TGGCCA
NdeI 2616 CATATG CA^TATG
2717 CATATG
PflMI 2754 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAAGTCCTGG
PpuMI 539 AGGACCC [GA]G^G[AT]CC[CT]
2601 AGGTCCC
PshAI 1075 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2638 GACCACAGTC
Van91I 2754 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAAGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BamHI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NheI NlaIII NlaIV NspI PflFI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII