################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccI AccIII AclI AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI BbeI BbrPI BciVI BclI BfrI BsaAI BseAI BsiWI BsmBI BspDI BspEI BspHI BssSI Bst1107I BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco81I EcoRI EcoRV EcoT22I FbaI FseI KasI MfeI MluI MroI MunI NarI NcoI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1012 GACGTC GACGT^C Acc65I 1532 GGTACC G^GTACC AcyI 1012 GACGTC G[GA]^CG[CT]C AhdI 1513 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 107 AGCGCT AGC^GCT Asp700I 2693 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1532 GGTACC G^GTACC AspEI 1513 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 710 TGCGCA TGC^GCA AvrII 2631 CCTAGG C^CTAGG BanI 1532 GGTACC G^G[CT][GA]CC BbvCI 1451 CCTCAGC CCTCAGC(-5/-2) BcgI 1359 CGAGGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1313 GCCGAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1056 AGATCT A^GATCT BlnI 2631 CCTAGG C^CTAGG BsaBI 1230 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1012 GACGTC G[GA]^CG[CT]C BsmI 1860 GCATTC GAATGC(1/-1) BsrBI 460 CCGCTC CCGCTC(-3/-3) BssHII 407 GCGCGC G^CGCGC BstBI 2983 TTCGAA TT^CGAA DsaI 402 CCGCGG C^C[GA][CT]GG EagI 399 CGGCCG C^GGCCG Eam1105I 1513 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 399 CGGCCG C^GGCCG Eco47III 107 AGCGCT AGC^GCT Eco52I 399 CGGCCG C^GGCCG FspI 710 TGCGCA TGC^GCA Hin1I 1012 GACGTC G[GA]^CG[CT]C HindIII 3615 AAGCTT A^AGCTT HpaI 1606 GTTAAC GTT^AAC KpnI 1532 GGTACC GGTAC^C KspI 402 CCGCGG CCGC^GG MamI 1230 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 398 GCGGCCGC GC^GGCCGC NspV 2983 TTCGAA TT^CGAA SacII 402 CCGCGG CCGC^GG SapI 720 GAAGAGC GCTCTTC(1/4) SexAI 3452 ACCAGGT A^CC[AT]GGT SfuI 2983 TTCGAA TT^CGAA SmaI 872 CCCGGG CCC^GGG SmlI 3254 CTTGAG C^T[CT][GA]AG SphI 2138 GCATGC GCATG^C StuI 1466 AGGCCT AGG^CCT XbaI 3752 TCTAGA T^CTAGA XmaCI 872 CCCGGG C^CCGGG XmaI 872 CCCGGG C^CCGGG XmnI 2693 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 1251 GAATTT [GA]^AATT[CT] 3140 AAATTC ApoI 1251 GAATTT [GA]^AATT[CT] 3140 AAATTC BalI 1481 TGGCCA TGG^CCA 3945 TGGCCA BlpI 2710 GCTTAGC GC^T[ACGT]AGC 3882 GCTCAGC Bpu1102I 2710 GCTTAGC GC^T[ACGT]AGC 3882 GCTCAGC BsiEI 399 CGGCCG CG[GA][CT]^CG 2071 CGACCG BspLU11I 3297 ACATGT A^CATGT 4022 ACATGT BspMI 653 ACCTGC ACCTGC(4/8) 3022 ACCTGC CelII 2710 GCTTAGC GC^T[ACGT]AGC 3882 GCTCAGC DraIII 2451 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3451 CACCAGGTG HaeII 107 AGCGCT [GA]GCGC^[CT] 274 AGCGCC HgaI 158 GCGTC GACGC(5/10) 725 GCGTC HincII 1606 GTTAAC GT[CT]^[GA]AC 2365 GTCAAC HindII 1606 GTTAAC GT[CT]^[GA]AC 2365 GTCAAC MluNI 1481 TGGCCA TGG^CCA 3945 TGGCCA MscI 1481 TGGCCA TGG^CCA 3945 TGGCCA NdeI 2616 CATATG CA^TATG 2717 CATATG PflMI 2754 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3467 CCAAGTCCTGG PpuMI 539 AGGACCC [GA]G^G[AT]CC[CT] 2601 AGGTCCC PshAI 1075 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2638 GACCACAGTC Van91I 2754 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3467 CCAAGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII BamHI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NheI NlaIII NlaIV NspI PflFI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII