################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AccIII AclI AgeI AhdI Aor51HI AscI AspEI AspI AvrII BbeI BbrPI BbvCI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII BssSI BstBI CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI HgaI HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI Sse8387I SspI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 2148 GACGTC GACGT^C Acc65I 2566 GGTACC G^GTACC AcyI 2148 GACGTC G[GA]^CG[CT]C AflII 914 CTTAAG C^TTAAG AflIII 3698 ACACGT A^C[GA][CT]GT Alw44I 2975 GTGCAC G^TGCAC ApaI 449 GGGCCC GGGCC^C ApaLI 2975 GTGCAC G^TGCAC Asp718I 2566 GGTACC G^GTACC AviII 216 TGCGCA TGC^GCA BamHI 607 GGATCC G^GATCC BclI 93 TGATCA T^GATCA BfrI 914 CTTAAG C^TTAAG BsaAI 1875 TACGTA [CT]AC^GT[GA] BsaBI 30 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2148 GACGTC G[GA]^CG[CT]C BsgI 1685 GTGCAG GTGCAG(16/14) BsmBI 2209 CGTCTC CGTCTC(1/5) BsmI 977 GAATGC GAATGC(1/-1) BspHI 96 TCATGA T^CATGA BsrBI 109 GAGCGG CCGCTC(-3/-3) BsrDI 598 CATTGC GCAATG(2/0) BsrFI 195 ACCGGC [GA]^CCGG[CT] BstEII 1851 GGTCACC G^GT[ACGT]ACC BstPI 1851 GGTCACC G^GT[ACGT]ACC Cfr10I 195 ACCGGC [GA]^CCGG[CT] DraIII 3657 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 639 GACTAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 234 CCACGG C^C[GA][CT]GG EcoNI 1544 CCTTGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1851 GGTCACC G^GT[ACGT]ACC FauI 136 CCCGC CCCGC(4/6) FbaI 93 TGATCA T^GATCA FspI 216 TGCGCA TGC^GCA Hin1I 2148 GACGTC G[GA]^CG[CT]C HindIII 1377 AAGCTT A^AGCTT KpnI 2566 GGTACC GGTAC^C MamI 30 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflMI 684 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 3278 CAGCTG CAG^CTG RcaI 96 TCATGA T^CATGA SalI 1619 GTCGAC G^TCGAC SapI 175 GAAGAGC GCTCTTC(1/4) ScaI 1987 AGTACT AGT^ACT SnaBI 1875 TACGTA TAC^GTA SpeI 2792 ACTAGT A^CTAGT SphI 3333 GCATGC GCATG^C Van91I 684 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2691 TCTAGA T^CTAGA XcmI 982 CCATCTATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 66 CGCG CG^CG 129 CGCG AocI 1086 CCTCAGG CC^T[ACGT]AGG 1452 CCTCAGG AseI 1880 ATTAAT AT^TAAT 1943 ATTAAT AsnI 1880 ATTAAT AT^TAAT 1943 ATTAAT AvaI 169 CCCGAG C^[CT]CG[GA]G 1009 CCCGAG BalI 692 TGGCCA TGG^CCA 2120 TGGCCA BsaI 680 GAGACC GGTCTC(1/5) 1736 GGTCTC BsmFI 1790 GGGAC GGGAC(10/14) 792 GTCCC BsoBI 169 CCCGAG C^[CT]CG[GA]G 1009 CCCGAG Bsp1407I 1079 TGTACA T^GTACA 2937 TGTACA BsrGI 1079 TGTACA T^GTACA 2937 TGTACA BstUI 66 CGCG CG^CG 129 CGCG BstYI 607 GGATCC [GA]^GATC[CT] 2880 GGATCT Bsu36I 1086 CCTCAGG CC^T[ACGT]AGG 1452 CCTCAGG DraI 2045 TTTAAA TTT^AAA 3643 TTTAAA EaeI 692 TGGCCA [CT]^GGCC[GA] 2120 TGGCCA EarI 817 CTCTTC CTCTTC(1/4) 175 GAAGAG Eco81I 1086 CCTCAGG CC^T[ACGT]AGG 1452 CCTCAGG EcoRI 2888 GAATTC G^AATTC 3067 GAATTC EcoT22I 2623 ATGCAT ATGCA^T 3331 ATGCAT HaeII 1502 AGCGCC [GA]GCGC^[CT] 2197 AGCGCC Ksp632I 817 CTCTTC CTCTTC(1/4) 175 GAAGAG MflI 607 GGATCC [GA]^GATC[CT] 2880 GGATCT MluNI 692 TGGCCA TGG^CCA 2120 TGGCCA MscI 692 TGGCCA TGG^CCA 2120 TGGCCA MvnI 66 CGCG CG^CG 129 CGCG NdeI 688 CATATG CA^TATG 2620 CATATG NheI 1020 GCTAGC G^CTAGC 1255 GCTAGC NsiI 2623 ATGCAT ATGCA^T 3331 ATGCAT NspI 343 ACATGC [GA]CATG^[CT] 3333 GCATGC PshBI 1880 ATTAAT AT^TAAT 1943 ATTAAT SacI 75 GAGCTC GAGCT^C 1598 GAGCTC SexAI 803 ACCAGGT A^CC[AT]GGT 2717 ACCTGGT SspBI 1079 TGTACA T^GTACA 2937 TGTACA XhoII 607 GGATCC [GA]^GATC[CT] 2880 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstXI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI