##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI AspI AvrII BbeI
BbrPI BbvCI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BssHII BssSI BstBI CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI HgaI
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SbfI SfiI SfoI
SfuI SgrAI SmaI Sse8387I SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2148 GACGTC GACGT^C
Acc65I 2566 GGTACC G^GTACC
AcyI 2148 GACGTC G[GA]^CG[CT]C
AflII 914 CTTAAG C^TTAAG
AflIII 3698 ACACGT A^C[GA][CT]GT
Alw44I 2975 GTGCAC G^TGCAC
ApaI 449 GGGCCC GGGCC^C
ApaLI 2975 GTGCAC G^TGCAC
Asp718I 2566 GGTACC G^GTACC
AviII 216 TGCGCA TGC^GCA
BamHI 607 GGATCC G^GATCC
BclI 93 TGATCA T^GATCA
BfrI 914 CTTAAG C^TTAAG
BsaAI 1875 TACGTA [CT]AC^GT[GA]
BsaBI 30 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2148 GACGTC G[GA]^CG[CT]C
BsgI 1685 GTGCAG GTGCAG(16/14)
BsmBI 2209 CGTCTC CGTCTC(1/5)
BsmI 977 GAATGC GAATGC(1/-1)
BspHI 96 TCATGA T^CATGA
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BsrDI 598 CATTGC GCAATG(2/0)
BsrFI 195 ACCGGC [GA]^CCGG[CT]
BstEII 1851 GGTCACC G^GT[ACGT]ACC
BstPI 1851 GGTCACC G^GT[ACGT]ACC
Cfr10I 195 ACCGGC [GA]^CCGG[CT]
DraIII 3657 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 639 GACTAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 234 CCACGG C^C[GA][CT]GG
EcoNI 1544 CCTTGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1851 GGTCACC G^GT[ACGT]ACC
FauI 136 CCCGC CCCGC(4/6)
FbaI 93 TGATCA T^GATCA
FspI 216 TGCGCA TGC^GCA
Hin1I 2148 GACGTC G[GA]^CG[CT]C
HindIII 1377 AAGCTT A^AGCTT
KpnI 2566 GGTACC GGTAC^C
MamI 30 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 684 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 3278 CAGCTG CAG^CTG
RcaI 96 TCATGA T^CATGA
SalI 1619 GTCGAC G^TCGAC
SapI 175 GAAGAGC GCTCTTC(1/4)
ScaI 1987 AGTACT AGT^ACT
SnaBI 1875 TACGTA TAC^GTA
SpeI 2792 ACTAGT A^CTAGT
SphI 3333 GCATGC GCATG^C
Van91I 684 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2691 TCTAGA T^CTAGA
XcmI 982 CCATCTATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 66 CGCG CG^CG
129 CGCG
AocI 1086 CCTCAGG CC^T[ACGT]AGG
1452 CCTCAGG
AseI 1880 ATTAAT AT^TAAT
1943 ATTAAT
AsnI 1880 ATTAAT AT^TAAT
1943 ATTAAT
AvaI 169 CCCGAG C^[CT]CG[GA]G
1009 CCCGAG
BalI 692 TGGCCA TGG^CCA
2120 TGGCCA
BsaI 680 GAGACC GGTCTC(1/5)
1736 GGTCTC
BsmFI 1790 GGGAC GGGAC(10/14)
792 GTCCC
BsoBI 169 CCCGAG C^[CT]CG[GA]G
1009 CCCGAG
Bsp1407I 1079 TGTACA T^GTACA
2937 TGTACA
BsrGI 1079 TGTACA T^GTACA
2937 TGTACA
BstUI 66 CGCG CG^CG
129 CGCG
BstYI 607 GGATCC [GA]^GATC[CT]
2880 GGATCT
Bsu36I 1086 CCTCAGG CC^T[ACGT]AGG
1452 CCTCAGG
DraI 2045 TTTAAA TTT^AAA
3643 TTTAAA
EaeI 692 TGGCCA [CT]^GGCC[GA]
2120 TGGCCA
EarI 817 CTCTTC CTCTTC(1/4)
175 GAAGAG
Eco81I 1086 CCTCAGG CC^T[ACGT]AGG
1452 CCTCAGG
EcoRI 2888 GAATTC G^AATTC
3067 GAATTC
EcoT22I 2623 ATGCAT ATGCA^T
3331 ATGCAT
HaeII 1502 AGCGCC [GA]GCGC^[CT]
2197 AGCGCC
Ksp632I 817 CTCTTC CTCTTC(1/4)
175 GAAGAG
MflI 607 GGATCC [GA]^GATC[CT]
2880 GGATCT
MluNI 692 TGGCCA TGG^CCA
2120 TGGCCA
MscI 692 TGGCCA TGG^CCA
2120 TGGCCA
MvnI 66 CGCG CG^CG
129 CGCG
NdeI 688 CATATG CA^TATG
2620 CATATG
NheI 1020 GCTAGC G^CTAGC
1255 GCTAGC
NsiI 2623 ATGCAT ATGCA^T
3331 ATGCAT
NspI 343 ACATGC [GA]CATG^[CT]
3333 GCATGC
PshBI 1880 ATTAAT AT^TAAT
1943 ATTAAT
SacI 75 GAGCTC GAGCT^C
1598 GAGCTC
SexAI 803 ACCAGGT A^CC[AT]GGT
2717 ACCTGGT
SspBI 1079 TGTACA T^GTACA
2937 TGTACA
XhoII 607 GGATCC [GA]^GATC[CT]
2880 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstXI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI