##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
Alw44I ApaI ApaLI AscI Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BfrI BglI
BlnI BsaBI BsaI BseAI BsiWI
BspDI BspEI BspMI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraIII EaeI EagI EclXI Eco52I
EcoO65I FseI FspI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI Sse8387I SwaI Tth111I Van91I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 225 GACGTC GACGT^C
AclI 4189 AACGTT AA^CGTT
AflIII 3321 ACATGT A^C[GA][CT]GT
AhdI 724 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1598 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2903 AGCGCT AGC^GCT
AspEI 724 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1266 GAGCTC G[AT]GC[AT]^C
BanI 2820 GGCACC G^G[CT][GA]CC
BlpI 1066 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1066 GCTCAGC GC^T[ACGT]AGC
BsaAI 1385 TACGTA [CT]AC^GT[GA]
BsaWI 1985 ACCGGA [AT]^CCGG[AT]
BseRI 2386 GAGGAG GAGGAG(10/8)
BsiEI 1788 CGGTCG CG[GA][CT]^CG
BsiHKAI 1266 GAGCTC G[AT]GC[AT]^C
BsmBI 3406 GAGACG CGTCTC(1/5)
BsmI 2325 GAATGC GAATGC(1/-1)
BspLU11I 3321 ACATGT A^CATGT
BssSI 525 CACGAG CACGAG(-5/-1)
Bsu36I 1598 CCTCAGG CC^T[ACGT]AGG
CelII 1066 GCTCAGC GC^T[ACGT]AGC
DraII 3523 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1246 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 724 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2903 AGCGCT AGC^GCT
Eco81I 1598 CCTCAGG CC^T[ACGT]AGG
EcoNI 1859 CCTGGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3523 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 195 GAATTC G^AATTC
EcoRV 3808 GATATC GAT^ATC
HgaI 2241 GACGC GACGC(5/10)
HindIII 944 AAGCTT A^AGCTT
HpaI 4186 GTTAAC GTT^AAC
MslI 1410 CACTCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2440 CCATGG C^CATGG
PpuMI 3523 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 4189 AACGTT AA^CGTT
PvuII 1949 CAGCTG CAG^CTG
SacI 1266 GAGCTC GAGCT^C
ScaI 1423 AGTACT AGT^ACT
SnaBI 1385 TACGTA TAC^GTA
SpeI 499 ACTAGT A^CTAGT
SphI 1415 GCATGC GCATG^C
SspI 1965 AATATT AAT^ATT
XbaI 327 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 225 GACGTC G[GA]^CG[CT]C
2241 GACGCC
AseI 3025 ATTAAT AT^TAAT
3735 ATTAAT
AsnI 3025 ATTAAT AT^TAAT
3735 ATTAAT
AvaII 3485 GGTCC G^G[AT]CC
3524 GGACC
BbsI 102 GTCTTC GAAGAC(2/6)
2526 GTCTTC
BciVI 264 GGATAC GTATCC(6/5)
2351 GTATCC
BclI 1830 TGATCA T^GATCA
3377 TGATCA
BcnI 908 CCCGG CC^[GC]GG
932 CCCGG
BglII 1536 AGATCT A^GATCT
1680 AGATCT
BpuAI 102 GTCTTC GAAGAC(2/6)
2526 GTCTTC
BsaHI 225 GACGTC G[GA]^CG[CT]C
2241 GACGCC
BsgI 1145 CTGCAC GTGCAG(16/14)
637 GTGCAG
BsmFI 2137 GGGAC GGGAC(10/14)
2032 GTCCC
Bsp1407I 2829 TGTACA T^GTACA
3102 TGTACA
BsrBI 594 CCGCTC CCGCTC(-3/-3)
1173 GAGCGG
BsrDI 1548 CATTGC GCAATG(2/0)
1435 GCAATG
BsrGI 2829 TGTACA T^GTACA
3102 TGTACA
BstAPI 979 GCATCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1435 GCAATGCCTGC
DsaI 898 CCACGG C^C[GA][CT]GG
2440 CCATGG
FauI 2824 CCCGC CCCGC(4/6)
2290 GCGGG
FbaI 1830 TGATCA T^GATCA
3377 TGATCA
HaeII 1307 GGCGCT [GA]GCGC^[CT]
2903 AGCGCT
Hin1I 225 GACGTC G[GA]^CG[CT]C
2241 GACGCC
MspA1I 1949 CAGCTG C[AC]G^C[GT]G
2825 CCGCTG
NciI 908 CCCGG CC^[GC]GG
932 CCCGG
NspI 1415 GCATGC [GA]CATG^[CT]
3321 ACATGT
PaeR7I 1217 CTCGAG C^TCGAG
1803 CTCGAG
PshBI 3025 ATTAAT AT^TAAT
3735 ATTAAT
SapI 926 GAAGAGC GCTCTTC(1/4)
3858 GCTCTTC
SmlI 1217 CTCGAG C^T[CT][GA]AG
1803 CTCGAG
SspBI 2829 TGTACA T^GTACA
3102 TGTACA
XhoI 1217 CTCGAG C^TCGAG
1803 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaI
BanII BbvCI BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsoBI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI PleI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI