##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BanI BanII
BbeI BbvCI BcgI BciVI BclI
BcnI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmBI
BspDI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I FbaI FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MamI MluI MspA1I
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI PacI PflFI
PflMI PinAI PmeI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I StyI SwaI Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 73 CGCG CG^CG
AccIII 2754 TCCGGA T^CCGGA
Aor51HI 870 AGCGCT AGC^GCT
AseI 3509 ATTAAT AT^TAAT
AsnI 3509 ATTAAT AT^TAAT
AvaI 1075 CTCGAG C^[CT]CG[GA]G
BamHI 3200 GGATCC G^GATCC
BbrPI 2913 CACGTG CAC^GTG
BbsI 4098 GTCTTC GAAGAC(2/6)
BbvI 4044 GCAGC GCAGC(8/12)
BglII 3180 AGATCT A^GATCT
BpuAI 4098 GTCTTC GAAGAC(2/6)
BsaAI 2913 CACGTG [CT]AC^GT[GA]
BsaI 3141 GAGACC GGTCTC(1/5)
BsaWI 2754 TCCGGA [AT]^CCGG[AT]
BseAI 2754 TCCGGA T^CCGGA
BsoBI 1075 CTCGAG C^[CT]CG[GA]G
BspEI 2754 TCCGGA T^CCGGA
BstAPI 4364 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2144 TTCGAA TT^CGAA
BstUI 73 CGCG CG^CG
DraI 3327 TTTAAA TTT^AAA
DraII 4380 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 213 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 870 AGCGCT AGC^GCT
EcoO109I 4380 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 4431 ATGCAT ATGCA^T
FauI 2197 GCGGG CCCGC(4/6)
Fnu4HI 4044 GCAGC GC^[ACGT]GC
HaeII 870 AGCGCT [GA]GCGC^[CT]
HapII 2755 CCGG C^CGG
HindIII 4062 AAGCTT A^AGCTT
HpaII 2755 CCGG C^CGG
ItaI 4044 GCAGC GC^[ACGT]GC
MroI 2754 TCCGGA T^CCGGA
MspI 2755 CCGG C^CGG
MvnI 73 CGCG CG^CG
NdeI 4181 CATATG CA^TATG
NsiI 4431 ATGCAT ATGCA^T
NspV 2144 TTCGAA TT^CGAA
PaeR7I 1075 CTCGAG C^TCGAG
PmaCI 2913 CACGTG CAC^GTG
PmlI 2913 CACGTG CAC^GTG
PpuMI 4380 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 3509 ATTAAT AT^TAAT
PstI 139 CTGCAG CTGCA^G
ScaI 4325 AGTACT AGT^ACT
SexAI 250 ACCTGGT A^CC[AT]GGT
SfuI 2144 TTCGAA TT^CGAA
SpeI 27 ACTAGT A^CTAGT
TseI 4044 GCAGC G^C[AT]GC
XbaI 4524 TCTAGA T^CTAGA
XcmI 4350 CCATGATTTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1075 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 3201 GATCC GGATC(4/5)
3200 GGATC
AspHI 787 GTGCTC G[AT]GC[AT]^C
2716 GTGCTC
BalI 304 TGGCCA TGG^CCA
3836 TGGCCA
BmyI 787 GTGCTC G[AGT]GC[ACT]^C
2716 GTGCTC
BseRI 144 GAGGAG GAGGAG(10/8)
3288 GAGGAG
BsgI 23 CTGCAC GTGCAG(16/14)
3597 CTGCAC
BsiHKAI 787 GTGCTC G[AT]GC[AT]^C
2716 GTGCTC
BsmFI 4382 GTCCC GGGAC(10/14)
4492 GTCCC
Bsp1286I 787 GTGCTC G[AGT]GC[ACT]^C
2716 GTGCTC
BspLU11I 3613 ACATGT A^CATGT
4543 ACATGT
BstYI 3180 AGATCT [GA]^GATC[CT]
3200 GGATCC
CfoI 74 GCGC GCG^C
871 GCGC
DpnII 3181 GATC ^GATC
3201 GATC
HhaI 74 GCGC GCG^C
871 GCGC
HinP1I 74 GCGC G^CGC
871 GCGC
HincII 3647 GTCAAC GT[CT]^[GA]AC
3946 GTTGAC
HindII 3647 GTCAAC GT[CT]^[GA]AC
3946 GTTGAC
MboI 3181 GATC ^GATC
3201 GATC
MfeI 3195 CAATTG C^AATTG
4388 CAATTG
MflI 3180 AGATCT [GA]^GATC[CT]
3200 GGATCC
MluNI 304 TGGCCA TGG^CCA
3836 TGGCCA
MscI 304 TGGCCA TGG^CCA
3836 TGGCCA
MunI 3195 CAATTG C^AATTG
4388 CAATTG
NdeII 3181 GATC ^GATC
3201 GATC
NspI 3613 ACATGT [GA]CATG^[CT]
4543 ACATGT
Sau3AI 3181 GATC ^GATC
3201 GATC
SspI 3422 AATATT AAT^ATT
3631 AATATT
XhoII 3180 AGATCT [GA]^GATC[CT]
3200 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI ApoI Asp700I AvaII BfaI
BfrI BpmI BsaJI BsiYI BslI
BsmAI BsmI Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EaeI EarI EcoRI
EcoRII FokI HaeIII HinfI HphI
Ksp632I MaeI MaeII MaeIII MboII
MnlI MseI MslI MvaI MwoI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XmnI