##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccIII AgeI Aor51HI ApaI AscI
AseI AsnI AviII BbeI BglII
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI FseI FspI
HaeII KasI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 4352 CGCG CG^CG
AclI 1122 AACGTT AA^CGTT
AflII 3806 CTTAAG C^TTAAG
AhdI 2831 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 583 GTGCAC G^TGCAC
ApaLI 583 GTGCAC G^TGCAC
AspEI 2831 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1793 CCCGGG C^[CT]CG[GA]G
AvrII 209 CCTAGG C^CTAGG
BalI 3604 TGGCCA TGG^CCA
BbrPI 3197 CACGTG CAC^GTG
BcgI 97 CGAAGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 772 GGATAC GTATCC(6/5)
BclI 1262 TGATCA T^GATCA
BfrI 3806 CTTAAG C^TTAAG
BlnI 209 CCTAGG C^CTAGG
BsaAI 3197 CACGTG [CT]AC^GT[GA]
BsaBI 1605 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 134 TCCGGT [AT]^CCGG[AT]
BsoBI 1793 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3185 TGTACA T^GTACA
BspLU11I 728 ACATGT A^CATGT
BsrGI 3185 TGTACA T^GTACA
BstUI 4352 CGCG CG^CG
DraI 682 TTTAAA TTT^AAA
DraIII 3270 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3108 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2831 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 945 GATATC GAT^ATC
FauI 2145 CCCGC CCCGC(4/6)
FbaI 1262 TGATCA T^GATCA
HindIII 3562 AAGCTT A^AGCTT
HpaI 4342 GTTAAC GTT^AAC
MamI 1605 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3604 TGGCCA TGG^CCA
MscI 3604 TGGCCA TGG^CCA
MvnI 4352 CGCG CG^CG
NdeI 1096 CATATG CA^TATG
PmaCI 3197 CACGTG CAC^GTG
PmlI 3197 CACGTG CAC^GTG
Psp1406I 1122 AACGTT AA^CGTT
SalI 955 GTCGAC G^TCGAC
SexAI 4314 ACCAGGT A^CC[AT]GGT
SmaI 1793 CCCGGG CCC^GGG
SmlI 3806 CTTAAG C^T[CT][GA]AG
SphI 3862 GCATGC GCATG^C
SspBI 3185 TGTACA T^GTACA
XbaI 2667 TCTAGA T^CTAGA
XmaCI 1793 CCCGGG C^CCGGG
XmaI 1793 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 236 GACGTC GACGT^C
1819 GACGTC
Acc65I 599 GGTACC G^GTACC
3838 GGTACC
AcsI 872 GAATTT [GA]^AATT[CT]
1490 AAATTT
AcyI 236 GACGTC G[GA]^CG[CT]C
1819 GACGTC
ApoI 872 GAATTT [GA]^AATT[CT]
1490 AAATTT
Asp718I 599 GGTACC G^GTACC
3838 GGTACC
BbsI 1628 GTCTTC GAAGAC(2/6)
4057 GTCTTC
BbvCI 1827 CCTCAGC CCTCAGC(-5/-2)
3994 GCTGAGG
BglI 1281 GCCATACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3778 GCCAGCTGGGC
BlpI 3520 GCTGAGC GC^T[ACGT]AGC
3564 GCTTAGC
Bpu1102I 3520 GCTGAGC GC^T[ACGT]AGC
3564 GCTTAGC
BpuAI 1628 GTCTTC GAAGAC(2/6)
4057 GTCTTC
BsaHI 236 GACGTC G[GA]^CG[CT]C
1819 GACGTC
BssSI 4221 CACGAG CACGAG(-5/-1)
1774 CTCGTG
BstAPI 609 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4120 GCAGCTGCTGC
BstXI 94 CCACGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2783 CCAGGGCACTGG
CelII 3520 GCTGAGC GC^T[ACGT]AGC
3564 GCTTAGC
EcoNI 4380 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4477 CCTCACCCAGG
EcoT22I 2492 ATGCAT ATGCA^T
2858 ATGCAT
HgaI 1689 GCGTC GACGC(5/10)
2373 GCGTC
Hin1I 236 GACGTC G[GA]^CG[CT]C
1819 GACGTC
KpnI 599 GGTACC GGTAC^C
3838 GGTACC
NsiI 2492 ATGCAT ATGCA^T
2858 ATGCAT
SacI 1879 GAGCTC GAGCT^C
2102 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AfaI AflIII AluI
AlwI AlwNI AocI Asp700I AspHI
AspI AvaII BamHI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI