################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AccIII AgeI Aor51HI ApaI AscI AseI AsnI AviII BbeI BglII BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HaeII KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 4352 CGCG CG^CG AclI 1122 AACGTT AA^CGTT AflII 3806 CTTAAG C^TTAAG AhdI 2831 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 583 GTGCAC G^TGCAC ApaLI 583 GTGCAC G^TGCAC AspEI 2831 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1793 CCCGGG C^[CT]CG[GA]G AvrII 209 CCTAGG C^CTAGG BalI 3604 TGGCCA TGG^CCA BbrPI 3197 CACGTG CAC^GTG BcgI 97 CGAAGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 772 GGATAC GTATCC(6/5) BclI 1262 TGATCA T^GATCA BfrI 3806 CTTAAG C^TTAAG BlnI 209 CCTAGG C^CTAGG BsaAI 3197 CACGTG [CT]AC^GT[GA] BsaBI 1605 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 134 TCCGGT [AT]^CCGG[AT] BsoBI 1793 CCCGGG C^[CT]CG[GA]G Bsp1407I 3185 TGTACA T^GTACA BspLU11I 728 ACATGT A^CATGT BsrGI 3185 TGTACA T^GTACA BstUI 4352 CGCG CG^CG DraI 682 TTTAAA TTT^AAA DraIII 3270 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3108 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2831 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 945 GATATC GAT^ATC FauI 2145 CCCGC CCCGC(4/6) FbaI 1262 TGATCA T^GATCA HindIII 3562 AAGCTT A^AGCTT HpaI 4342 GTTAAC GTT^AAC MamI 1605 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3604 TGGCCA TGG^CCA MscI 3604 TGGCCA TGG^CCA MvnI 4352 CGCG CG^CG NdeI 1096 CATATG CA^TATG PmaCI 3197 CACGTG CAC^GTG PmlI 3197 CACGTG CAC^GTG Psp1406I 1122 AACGTT AA^CGTT SalI 955 GTCGAC G^TCGAC SexAI 4314 ACCAGGT A^CC[AT]GGT SmaI 1793 CCCGGG CCC^GGG SmlI 3806 CTTAAG C^T[CT][GA]AG SphI 3862 GCATGC GCATG^C SspBI 3185 TGTACA T^GTACA XbaI 2667 TCTAGA T^CTAGA XmaCI 1793 CCCGGG C^CCGGG XmaI 1793 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 236 GACGTC GACGT^C 1819 GACGTC Acc65I 599 GGTACC G^GTACC 3838 GGTACC AcsI 872 GAATTT [GA]^AATT[CT] 1490 AAATTT AcyI 236 GACGTC G[GA]^CG[CT]C 1819 GACGTC ApoI 872 GAATTT [GA]^AATT[CT] 1490 AAATTT Asp718I 599 GGTACC G^GTACC 3838 GGTACC BbsI 1628 GTCTTC GAAGAC(2/6) 4057 GTCTTC BbvCI 1827 CCTCAGC CCTCAGC(-5/-2) 3994 GCTGAGG BglI 1281 GCCATACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3778 GCCAGCTGGGC BlpI 3520 GCTGAGC GC^T[ACGT]AGC 3564 GCTTAGC Bpu1102I 3520 GCTGAGC GC^T[ACGT]AGC 3564 GCTTAGC BpuAI 1628 GTCTTC GAAGAC(2/6) 4057 GTCTTC BsaHI 236 GACGTC G[GA]^CG[CT]C 1819 GACGTC BssSI 4221 CACGAG CACGAG(-5/-1) 1774 CTCGTG BstAPI 609 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4120 GCAGCTGCTGC BstXI 94 CCACGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2783 CCAGGGCACTGG CelII 3520 GCTGAGC GC^T[ACGT]AGC 3564 GCTTAGC EcoNI 4380 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4477 CCTCACCCAGG EcoT22I 2492 ATGCAT ATGCA^T 2858 ATGCAT HgaI 1689 GCGTC GACGC(5/10) 2373 GCGTC Hin1I 236 GACGTC G[GA]^CG[CT]C 1819 GACGTC KpnI 599 GGTACC GGTAC^C 3838 GGTACC NsiI 2492 ATGCAT ATGCA^T 2858 ATGCAT SacI 1879 GAGCTC GAGCT^C 2102 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AfaI AflIII AluI AlwI AlwNI AocI Asp700I AspHI AspI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI