##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        AocI        ApaI        AscI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbrPI       
BcgI        BlnI        BsaAI       BsaWI       BseAI       
BsiWI       BspDI       BspEI       BsrBI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DrdI        Eam1105I    Eco81I      EcoRV       
EcoT22I     FseI        FspI        HpaI        KpnI        
MluI        MroI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Alw44I       4780  GTGCAC               G^TGCAC
Aor51HI      1226  AGCGCT               AGC^GCT
ApaLI        4780  GTGCAC               G^TGCAC
AspI          177  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         3474  GGCGCC               GGCGC^C
BciVI        2113  GTATCC               GTATCC(6/5)
BclI         2487  TGATCA               T^GATCA
BssSI        3531  CACGAG               CACGAG(-5/-1)
Eco47III     1226  AGCGCT               AGC^GCT
FbaI         2487  TGATCA               T^GATCA
KasI         3474  GGCGCC               G^GCGCC
KspI           58  CCGCGG               CCGC^GG
MfeI         4284  CAATTG               C^AATTG
MunI         4284  CAATTG               C^AATTG
NaeI         3231  GCCGGC               GCC^GGC
NarI         3474  GGCGCC               GG^CGCC
NcoI         2342  CCATGG               C^CATGG
NgoMIV       3231  GCCGGC               G^CCGGC
PflFI         177  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4553  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        3156  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          58  CCGCGG               CCGC^GG
SalI          872  GTCGAC               G^TCGAC
ScaI         2149  AGTACT               AGT^ACT
SfoI         3474  GGCGCC               GGC^GCC
SmaI         3426  CCCGGG               CCC^GGG
StuI         3362  AGGCCT               AGG^CCT
Tth111I       177  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4553  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1284  CCATACCTCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        3426  CCCGGG               C^CCGGG
XmaI         3426  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII          59  CGCG                 CG^CG
              290  CGCG                
AcyI           54  GACGCC               G[GA]^CG[CT]C
             3474  GGCGCC              
AflII        1509  CTTAAG               C^TTAAG
             4021  CTTAAG              
AseI         3811  ATTAAT               AT^TAAT
             4941  ATTAAT              
AsnI         3811  ATTAAT               AT^TAAT
             4941  ATTAAT              
BalI         2952  TGGCCA               TGG^CCA
             3605  TGGCCA              
BanI         2298  GGCACC               G^G[CT][GA]CC
             3474  GGCGCC              
BbvCI        2176  CCTCAGC              CCTCAGC(-5/-2)
             2222  CCTCAGC             
BfrI         1509  CTTAAG               C^TTAAG
             4021  CTTAAG              
BsaHI          54  GACGCC               G[GA]^CG[CT]C
             3474  GGCGCC              
BsmBI        1991  CGTCTC               CGTCTC(1/5)
               81  GAGACG              
BsmI         2070  GAATGC               GAATGC(1/-1)
             2715  GAATGC              
Bsp1407I     1581  TGTACA               T^GTACA
             5133  TGTACA              
BspHI         620  TCATGA               T^CATGA
             1068  TCATGA              
BsrDI        2099  GCAATG               GCAATG(2/0)
             3845  GCAATG              
BsrFI         157  ACCGGC               [GA]^CCGG[CT]
             3231  GCCGGC              
BsrGI        1581  TGTACA               T^GTACA
             5133  TGTACA              
BstEII       1642  GGTCACC              G^GT[ACGT]ACC
             3103  GGTAACC             
BstPI        1642  GGTCACC              G^GT[ACGT]ACC
             3103  GGTAACC             
BstUI          59  CGCG                 CG^CG
              290  CGCG                
BstXI         645  CCATAAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2307  CCATATGTATGG        
Cfr10I        157  ACCGGC               [GA]^CCGG[CT]
             3231  GCCGGC              
DraIII       1645  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4754  CACTGTGTG           
EcoNI        1381  CCTGGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1723  CCTACAGCAGG         
EcoO65I      1642  GGTCACC              G^GT[ACGT]ACC
             3103  GGTAACC             
EcoRI        3760  GAATTC               G^AATTC
             4121  GAATTC              
HaeII        1226  AGCGCT               [GA]GCGC^[CT]
             3474  GGCGCC              
Hin1I          54  GACGCC               G[GA]^CG[CT]C
             3474  GGCGCC              
HincII        872  GTCGAC               GT[CT]^[GA]AC
             3590  GTTGAC              
HindII        872  GTCGAC               GT[CT]^[GA]AC
             3590  GTTGAC              
MaeII        2126  ACGT                 A^CGT
             2700  ACGT                
MluNI        2952  TGGCCA               TGG^CCA
             3605  TGGCCA              
MscI         2952  TGGCCA               TGG^CCA
             3605  TGGCCA              
MvnI           59  CGCG                 CG^CG
              290  CGCG                
PpuMI        3429  GGGACCC              [GA]G^G[AT]CC[CT]
             4365  GGGACCT             
PshBI        3811  ATTAAT               AT^TAAT
             4941  ATTAAT              
RcaI          620  TCATGA               T^CATGA
             1068  TCATGA              
SspBI        1581  TGTACA               T^GTACA
             5133  TGTACA              
SspI         4071  AATATT               AAT^ATT
             4905  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspLU11I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI