################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI AocI ApaI AscI Asp718I AspEI AviII AvrII BamHI BbrPI BcgI BlnI BsaAI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco81I EcoRV EcoT22I FseI FspI HpaI KpnI MluI MroI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Alw44I 4780 GTGCAC G^TGCAC Aor51HI 1226 AGCGCT AGC^GCT ApaLI 4780 GTGCAC G^TGCAC AspI 177 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 3474 GGCGCC GGCGC^C BciVI 2113 GTATCC GTATCC(6/5) BclI 2487 TGATCA T^GATCA BssSI 3531 CACGAG CACGAG(-5/-1) Eco47III 1226 AGCGCT AGC^GCT FbaI 2487 TGATCA T^GATCA KasI 3474 GGCGCC G^GCGCC KspI 58 CCGCGG CCGC^GG MfeI 4284 CAATTG C^AATTG MunI 4284 CAATTG C^AATTG NaeI 3231 GCCGGC GCC^GGC NarI 3474 GGCGCC GG^CGCC NcoI 2342 CCATGG C^CATGG NgoMIV 3231 GCCGGC G^CCGGC PflFI 177 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4553 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3156 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 58 CCGCGG CCGC^GG SalI 872 GTCGAC G^TCGAC ScaI 2149 AGTACT AGT^ACT SfoI 3474 GGCGCC GGC^GCC SmaI 3426 CCCGGG CCC^GGG StuI 3362 AGGCCT AGG^CCT Tth111I 177 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4553 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1284 CCATACCTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 3426 CCCGGG C^CCGGG XmaI 3426 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 59 CGCG CG^CG 290 CGCG AcyI 54 GACGCC G[GA]^CG[CT]C 3474 GGCGCC AflII 1509 CTTAAG C^TTAAG 4021 CTTAAG AseI 3811 ATTAAT AT^TAAT 4941 ATTAAT AsnI 3811 ATTAAT AT^TAAT 4941 ATTAAT BalI 2952 TGGCCA TGG^CCA 3605 TGGCCA BanI 2298 GGCACC G^G[CT][GA]CC 3474 GGCGCC BbvCI 2176 CCTCAGC CCTCAGC(-5/-2) 2222 CCTCAGC BfrI 1509 CTTAAG C^TTAAG 4021 CTTAAG BsaHI 54 GACGCC G[GA]^CG[CT]C 3474 GGCGCC BsmBI 1991 CGTCTC CGTCTC(1/5) 81 GAGACG BsmI 2070 GAATGC GAATGC(1/-1) 2715 GAATGC Bsp1407I 1581 TGTACA T^GTACA 5133 TGTACA BspHI 620 TCATGA T^CATGA 1068 TCATGA BsrDI 2099 GCAATG GCAATG(2/0) 3845 GCAATG BsrFI 157 ACCGGC [GA]^CCGG[CT] 3231 GCCGGC BsrGI 1581 TGTACA T^GTACA 5133 TGTACA BstEII 1642 GGTCACC G^GT[ACGT]ACC 3103 GGTAACC BstPI 1642 GGTCACC G^GT[ACGT]ACC 3103 GGTAACC BstUI 59 CGCG CG^CG 290 CGCG BstXI 645 CCATAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2307 CCATATGTATGG Cfr10I 157 ACCGGC [GA]^CCGG[CT] 3231 GCCGGC DraIII 1645 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 4754 CACTGTGTG EcoNI 1381 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1723 CCTACAGCAGG EcoO65I 1642 GGTCACC G^GT[ACGT]ACC 3103 GGTAACC EcoRI 3760 GAATTC G^AATTC 4121 GAATTC HaeII 1226 AGCGCT [GA]GCGC^[CT] 3474 GGCGCC Hin1I 54 GACGCC G[GA]^CG[CT]C 3474 GGCGCC HincII 872 GTCGAC GT[CT]^[GA]AC 3590 GTTGAC HindII 872 GTCGAC GT[CT]^[GA]AC 3590 GTTGAC MaeII 2126 ACGT A^CGT 2700 ACGT MluNI 2952 TGGCCA TGG^CCA 3605 TGGCCA MscI 2952 TGGCCA TGG^CCA 3605 TGGCCA MvnI 59 CGCG CG^CG 290 CGCG PpuMI 3429 GGGACCC [GA]G^G[AT]CC[CT] 4365 GGGACCT PshBI 3811 ATTAAT AT^TAAT 4941 ATTAAT RcaI 620 TCATGA T^CATGA 1068 TCATGA SspBI 1581 TGTACA T^GTACA 5133 TGTACA SspI 4071 AATATT AAT^ATT 4905 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI