##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI ApaI AscI Asp718I
AspEI AviII AvrII BamHI BbrPI
BcgI BlnI BsaAI BsaWI BseAI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DrdI Eam1105I Eco81I EcoRV
EcoT22I FseI FspI HpaI KpnI
MluI MroI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 4780 GTGCAC G^TGCAC
Aor51HI 1226 AGCGCT AGC^GCT
ApaLI 4780 GTGCAC G^TGCAC
AspI 177 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3474 GGCGCC GGCGC^C
BciVI 2113 GTATCC GTATCC(6/5)
BclI 2487 TGATCA T^GATCA
BssSI 3531 CACGAG CACGAG(-5/-1)
Eco47III 1226 AGCGCT AGC^GCT
FbaI 2487 TGATCA T^GATCA
KasI 3474 GGCGCC G^GCGCC
KspI 58 CCGCGG CCGC^GG
MfeI 4284 CAATTG C^AATTG
MunI 4284 CAATTG C^AATTG
NaeI 3231 GCCGGC GCC^GGC
NarI 3474 GGCGCC GG^CGCC
NcoI 2342 CCATGG C^CATGG
NgoMIV 3231 GCCGGC G^CCGGC
PflFI 177 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4553 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3156 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 58 CCGCGG CCGC^GG
SalI 872 GTCGAC G^TCGAC
ScaI 2149 AGTACT AGT^ACT
SfoI 3474 GGCGCC GGC^GCC
SmaI 3426 CCCGGG CCC^GGG
StuI 3362 AGGCCT AGG^CCT
Tth111I 177 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4553 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1284 CCATACCTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3426 CCCGGG C^CCGGG
XmaI 3426 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 59 CGCG CG^CG
290 CGCG
AcyI 54 GACGCC G[GA]^CG[CT]C
3474 GGCGCC
AflII 1509 CTTAAG C^TTAAG
4021 CTTAAG
AseI 3811 ATTAAT AT^TAAT
4941 ATTAAT
AsnI 3811 ATTAAT AT^TAAT
4941 ATTAAT
BalI 2952 TGGCCA TGG^CCA
3605 TGGCCA
BanI 2298 GGCACC G^G[CT][GA]CC
3474 GGCGCC
BbvCI 2176 CCTCAGC CCTCAGC(-5/-2)
2222 CCTCAGC
BfrI 1509 CTTAAG C^TTAAG
4021 CTTAAG
BsaHI 54 GACGCC G[GA]^CG[CT]C
3474 GGCGCC
BsmBI 1991 CGTCTC CGTCTC(1/5)
81 GAGACG
BsmI 2070 GAATGC GAATGC(1/-1)
2715 GAATGC
Bsp1407I 1581 TGTACA T^GTACA
5133 TGTACA
BspHI 620 TCATGA T^CATGA
1068 TCATGA
BsrDI 2099 GCAATG GCAATG(2/0)
3845 GCAATG
BsrFI 157 ACCGGC [GA]^CCGG[CT]
3231 GCCGGC
BsrGI 1581 TGTACA T^GTACA
5133 TGTACA
BstEII 1642 GGTCACC G^GT[ACGT]ACC
3103 GGTAACC
BstPI 1642 GGTCACC G^GT[ACGT]ACC
3103 GGTAACC
BstUI 59 CGCG CG^CG
290 CGCG
BstXI 645 CCATAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2307 CCATATGTATGG
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
3231 GCCGGC
DraIII 1645 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4754 CACTGTGTG
EcoNI 1381 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1723 CCTACAGCAGG
EcoO65I 1642 GGTCACC G^GT[ACGT]ACC
3103 GGTAACC
EcoRI 3760 GAATTC G^AATTC
4121 GAATTC
HaeII 1226 AGCGCT [GA]GCGC^[CT]
3474 GGCGCC
Hin1I 54 GACGCC G[GA]^CG[CT]C
3474 GGCGCC
HincII 872 GTCGAC GT[CT]^[GA]AC
3590 GTTGAC
HindII 872 GTCGAC GT[CT]^[GA]AC
3590 GTTGAC
MaeII 2126 ACGT A^CGT
2700 ACGT
MluNI 2952 TGGCCA TGG^CCA
3605 TGGCCA
MscI 2952 TGGCCA TGG^CCA
3605 TGGCCA
MvnI 59 CGCG CG^CG
290 CGCG
PpuMI 3429 GGGACCC [GA]G^G[AT]CC[CT]
4365 GGGACCT
PshBI 3811 ATTAAT AT^TAAT
4941 ATTAAT
RcaI 620 TCATGA T^CATGA
1068 TCATGA
SspBI 1581 TGTACA T^GTACA
5133 TGTACA
SspI 4071 AATATT AAT^ATT
4905 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI