##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AocI AscI Asp718I BamHI BclI
BfrI BglI BsiWI Bsp1407I BspDI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KpnI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 3252 TCCGGA T^CCGGA
AflIII 347 ACATGT A^C[GA][CT]GT
AhdI 294 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2795 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 294 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 42 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2857 TGCGCA TGC^GCA
AvrII 3952 CCTAGG C^CTAGG
BbeI 3491 GGCGCC GGCGC^C
BbrPI 2847 CACGTG CAC^GTG
BlnI 3952 CCTAGG C^CTAGG
BlpI 3011 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3011 GCTAAGC GC^T[ACGT]AGC
BsaBI 1811 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3252 TCCGGA T^CCGGA
BspEI 3252 TCCGGA T^CCGGA
BspLU11I 347 ACATGT A^CATGT
BstEII 2064 GGTCACC G^GT[ACGT]ACC
BstPI 2064 GGTCACC G^GT[ACGT]ACC
CelII 3011 GCTAAGC GC^T[ACGT]AGC
DraI 4406 TTTAAA TTT^AAA
DraIII 4269 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2549 GACCGTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2929 CGGCCG C^GGCCG
Eam1105I 294 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2929 CGGCCG C^GGCCG
Eco52I 2929 CGGCCG C^GGCCG
EcoO65I 2064 GGTCACC G^GT[ACGT]ACC
FspI 2857 TGCGCA TGC^GCA
HincII 4328 GTTGAC GT[CT]^[GA]AC
HindII 4328 GTTGAC GT[CT]^[GA]AC
KasI 3491 GGCGCC G^GCGCC
MamI 1811 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3252 TCCGGA T^CCGGA
NaeI 2927 GCCGGC GCC^GGC
NarI 3491 GGCGCC GG^CGCC
NgoMIV 2927 GCCGGC G^CCGGC
PflFI 42 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2847 CACGTG CAC^GTG
PmlI 2847 CACGTG CAC^GTG
PpuMI 1213 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1916 CTGCAG CTGCA^G
PvuI 2309 CGATCG CGAT^CG
SapI 362 GCTCTTC GCTCTTC(1/4)
SexAI 1183 ACCAGGT A^CC[AT]GGT
SfcI 1916 CTGCAG C^T[GA][CT]AG
SfoI 3491 GGCGCC GGC^GCC
SmaI 2607 CCCGGG CCC^GGG
Tth111I 42 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2607 CCCGGG C^CCGGG
XmaI 2607 CCCGGG C^CCGGG
XmnI 2795 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1146 CGCG CG^CG
2546 CGCG
AclI 1293 AACGTT AA^CGTT
3884 AACGTT
Alw44I 2387 GTGCAC G^TGCAC
2865 GTGCAC
AlwNI 609 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1440 CAGTGTCTG
Aor51HI 469 AGCGCT AGC^GCT
3968 AGCGCT
ApaI 1043 GGGCCC GGGCC^C
1626 GGGCCC
ApaLI 2387 GTGCAC G^TGCAC
2865 GTGCAC
AseI 2325 ATTAAT AT^TAAT
3195 ATTAAT
AsnI 2325 ATTAAT AT^TAAT
3195 ATTAAT
AvaI 2607 CCCGGG C^[CT]CG[GA]G
4214 CCCGAG
BbsI 2688 GTCTTC GAAGAC(2/6)
2967 GTCTTC
BciVI 935 GGATAC GTATCC(6/5)
4396 GGATAC
BpuAI 2688 GTCTTC GAAGAC(2/6)
2967 GTCTTC
BsaAI 486 TACGTG [CT]AC^GT[GA]
2847 CACGTG
BsaWI 226 ACCGGA [AT]^CCGG[AT]
3252 TCCGGA
BsgI 504 CTGCAC GTGCAG(16/14)
2883 GTGCAG
BsoBI 2607 CCCGGG C^[CT]CG[GA]G
4214 CCCGAG
BsrFI 946 ACCGGC [GA]^CCGG[CT]
2927 GCCGGC
BssSI 3630 CACGAG CACGAG(-5/-1)
4180 CTCGTG
BstAPI 608 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3716 GCAGCCTGTGC
BstUI 1146 CGCG CG^CG
2546 CGCG
Cfr10I 946 ACCGGC [GA]^CCGG[CT]
2927 GCCGGC
DraII 1213 GGGACCC [GA]G^G[ACGT]CC[CT]
1625 GGGGCCC
Eco47III 469 AGCGCT AGC^GCT
3968 AGCGCT
EcoNI 961 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1178 CCTGGACCAGG
EcoO109I 1213 GGGACCC [GA]G^G[ACGT]CC[CT]
1625 GGGGCCC
HindIII 2271 AAGCTT A^AGCTT
2796 AAGCTT
MvnI 1146 CGCG CG^CG
2546 CGCG
PshAI 2177 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2696 GACAGCTGTC
PshBI 2325 ATTAAT AT^TAAT
3195 ATTAAT
Psp1406I 1293 AACGTT AA^CGTT
3884 AACGTT
SacI 360 GAGCTC GAGCT^C
3793 GAGCTC
SmlI 2454 CTCAAG C^T[CT][GA]AG
2580 CTCAAG
TaqI 2312 TCGA T^CGA
2785 TCGA
TthHB8I 2312 TCGA T^CGA
2785 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BanI BanII BbvCI BbvI BcgI
BcnI BfaI BglII BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII