################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII Acc65I AccI AflII AgeI AocI AscI Asp718I BamHI BclI BfrI BglI BsiWI Bsp1407I BspDI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI Eco81I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 3252 TCCGGA T^CCGGA AflIII 347 ACATGT A^C[GA][CT]GT AhdI 294 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2795 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 294 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 42 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2857 TGCGCA TGC^GCA AvrII 3952 CCTAGG C^CTAGG BbeI 3491 GGCGCC GGCGC^C BbrPI 2847 CACGTG CAC^GTG BlnI 3952 CCTAGG C^CTAGG BlpI 3011 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3011 GCTAAGC GC^T[ACGT]AGC BsaBI 1811 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3252 TCCGGA T^CCGGA BspEI 3252 TCCGGA T^CCGGA BspLU11I 347 ACATGT A^CATGT BstEII 2064 GGTCACC G^GT[ACGT]ACC BstPI 2064 GGTCACC G^GT[ACGT]ACC CelII 3011 GCTAAGC GC^T[ACGT]AGC DraI 4406 TTTAAA TTT^AAA DraIII 4269 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2549 GACCGTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2929 CGGCCG C^GGCCG Eam1105I 294 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2929 CGGCCG C^GGCCG Eco52I 2929 CGGCCG C^GGCCG EcoO65I 2064 GGTCACC G^GT[ACGT]ACC FspI 2857 TGCGCA TGC^GCA HincII 4328 GTTGAC GT[CT]^[GA]AC HindII 4328 GTTGAC GT[CT]^[GA]AC KasI 3491 GGCGCC G^GCGCC MamI 1811 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3252 TCCGGA T^CCGGA NaeI 2927 GCCGGC GCC^GGC NarI 3491 GGCGCC GG^CGCC NgoMIV 2927 GCCGGC G^CCGGC PflFI 42 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2847 CACGTG CAC^GTG PmlI 2847 CACGTG CAC^GTG PpuMI 1213 GGGACCC [GA]G^G[AT]CC[CT] PstI 1916 CTGCAG CTGCA^G PvuI 2309 CGATCG CGAT^CG SapI 362 GCTCTTC GCTCTTC(1/4) SexAI 1183 ACCAGGT A^CC[AT]GGT SfcI 1916 CTGCAG C^T[GA][CT]AG SfoI 3491 GGCGCC GGC^GCC SmaI 2607 CCCGGG CCC^GGG Tth111I 42 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2607 CCCGGG C^CCGGG XmaI 2607 CCCGGG C^CCGGG XmnI 2795 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 1146 CGCG CG^CG 2546 CGCG AclI 1293 AACGTT AA^CGTT 3884 AACGTT Alw44I 2387 GTGCAC G^TGCAC 2865 GTGCAC AlwNI 609 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1440 CAGTGTCTG Aor51HI 469 AGCGCT AGC^GCT 3968 AGCGCT ApaI 1043 GGGCCC GGGCC^C 1626 GGGCCC ApaLI 2387 GTGCAC G^TGCAC 2865 GTGCAC AseI 2325 ATTAAT AT^TAAT 3195 ATTAAT AsnI 2325 ATTAAT AT^TAAT 3195 ATTAAT AvaI 2607 CCCGGG C^[CT]CG[GA]G 4214 CCCGAG BbsI 2688 GTCTTC GAAGAC(2/6) 2967 GTCTTC BciVI 935 GGATAC GTATCC(6/5) 4396 GGATAC BpuAI 2688 GTCTTC GAAGAC(2/6) 2967 GTCTTC BsaAI 486 TACGTG [CT]AC^GT[GA] 2847 CACGTG BsaWI 226 ACCGGA [AT]^CCGG[AT] 3252 TCCGGA BsgI 504 CTGCAC GTGCAG(16/14) 2883 GTGCAG BsoBI 2607 CCCGGG C^[CT]CG[GA]G 4214 CCCGAG BsrFI 946 ACCGGC [GA]^CCGG[CT] 2927 GCCGGC BssSI 3630 CACGAG CACGAG(-5/-1) 4180 CTCGTG BstAPI 608 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3716 GCAGCCTGTGC BstUI 1146 CGCG CG^CG 2546 CGCG Cfr10I 946 ACCGGC [GA]^CCGG[CT] 2927 GCCGGC DraII 1213 GGGACCC [GA]G^G[ACGT]CC[CT] 1625 GGGGCCC Eco47III 469 AGCGCT AGC^GCT 3968 AGCGCT EcoNI 961 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1178 CCTGGACCAGG EcoO109I 1213 GGGACCC [GA]G^G[ACGT]CC[CT] 1625 GGGGCCC HindIII 2271 AAGCTT A^AGCTT 2796 AAGCTT MvnI 1146 CGCG CG^CG 2546 CGCG PshAI 2177 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2696 GACAGCTGTC PshBI 2325 ATTAAT AT^TAAT 3195 ATTAAT Psp1406I 1293 AACGTT AA^CGTT 3884 AACGTT SacI 360 GAGCTC GAGCT^C 3793 GAGCTC SmlI 2454 CTCAAG C^T[CT][GA]AG 2580 CTCAAG TaqI 2312 TCGA T^CGA 2785 TCGA TthHB8I 2312 TCGA T^CGA 2785 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BalI BanI BanII BbvCI BbvI BcgI BcnI BfaI BglII BmyI BpmI BsaHI BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII