##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII BamHI
BcgI BciVI BfrI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
Eam1105I Eco47III EcoO65I FseI FspI
KspI MamI MluI MroI NaeI
NgoMIV NruI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 949 GACGTC GACGT^C
AccI 2932 GTAGAC GT^[AC][GT]AC
AcsI 2365 GAATTC [GA]^AATT[CT]
AlwI 1789 GATCC GGATC(4/5)
AocI 154 CCTGAGG CC^T[ACGT]AGG
ApaI 3094 GGGCCC GGGCC^C
ApoI 2365 GAATTC [GA]^AATT[CT]
AvrII 2773 CCTAGG C^CTAGG
BbeI 3982 GGCGCC GGCGC^C
BglII 1031 AGATCT A^GATCT
BlnI 2773 CCTAGG C^CTAGG
BsaI 1083 GGTCTC GGTCTC(1/5)
BsiEI 3158 CGGCCG CG[GA][CT]^CG
Bsp1407I 3009 TGTACA T^GTACA
BsrGI 3009 TGTACA T^GTACA
BssHII 114 GCGCGC G^CGCGC
BstYI 1031 AGATCT [GA]^GATC[CT]
Bsu36I 154 CCTGAGG CC^T[ACGT]AGG
DraI 2988 TTTAAA TTT^AAA
EagI 3158 CGGCCG C^GGCCG
EclXI 3158 CGGCCG C^GGCCG
Eco52I 3158 CGGCCG C^GGCCG
Eco81I 154 CCTGAGG CC^T[ACGT]AGG
EcoNI 1855 CCTGCACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2365 GAATTC G^AATTC
EcoRV 2474 GATATC GAT^ATC
HincII 1540 GTTAAC GT[CT]^[GA]AC
HindII 1540 GTTAAC GT[CT]^[GA]AC
HindIII 3456 AAGCTT A^AGCTT
HpaI 1540 GTTAAC GTT^AAC
KasI 3982 GGCGCC G^GCGCC
MflI 1031 AGATCT [GA]^GATC[CT]
NarI 3982 GGCGCC GG^CGCC
NdeI 1406 CATATG CA^TATG
NheI 3114 GCTAGC G^CTAGC
NotI 3157 GCGGCCGC GC^GGCCGC
PaeR7I 3187 CTCGAG C^TCGAG
PflMI 3487 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4000 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3449 GAGCTC GAGCT^C
SexAI 872 ACCTGGT A^CC[AT]GGT
SfoI 3982 GGCGCC GGC^GCC
SmaI 119 CCCGGG CCC^GGG
SspBI 3009 TGTACA T^GTACA
StuI 3536 AGGCCT AGG^CCT
SwaI 2987 ATTTAAAT ATTT^AAAT
Van91I 3487 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3187 CTCGAG C^TCGAG
XhoII 1031 AGATCT [GA]^GATC[CT]
XmaCI 119 CCCGGG C^CCGGG
XmaI 119 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 186 GGTACC G^GTACC
1670 GGTACC
Asp700I 3417 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3646 GAAAGCATTC
Asp718I 186 GGTACC G^GTACC
1670 GGTACC
AvaI 119 CCCGGG C^[CT]CG[GA]G
3187 CTCGAG
BbsI 373 GTCTTC GAAGAC(2/6)
1966 GTCTTC
BbvCI 3767 CCTCAGC CCTCAGC(-5/-2)
2604 GCTGAGG
BclI 668 TGATCA T^GATCA
2411 TGATCA
BlpI 175 GCTGAGC GC^T[ACGT]AGC
3321 GCTGAGC
Bpu1102I 175 GCTGAGC GC^T[ACGT]AGC
3321 GCTGAGC
BpuAI 373 GTCTTC GAAGAC(2/6)
1966 GTCTTC
BsmI 1821 GCATTC GAATGC(1/-1)
3650 GCATTC
BsoBI 119 CCCGGG C^[CT]CG[GA]G
3187 CTCGAG
BspHI 692 TCATGA T^CATGA
2728 TCATGA
BsrFI 347 ACCGGC [GA]^CCGG[CT]
959 ACCGGC
BstXI 3347 CCATGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3714 CCAACTCTATGG
CelII 175 GCTGAGC GC^T[ACGT]AGC
3321 GCTGAGC
Cfr10I 347 ACCGGC [GA]^CCGG[CT]
959 ACCGGC
DraIII 871 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4115 CACCATGTG
EcoT22I 1199 ATGCAT ATGCA^T
3735 ATGCAT
FbaI 668 TGATCA T^GATCA
2411 TGATCA
HaeII 711 GGCGCT [GA]GCGC^[CT]
3982 GGCGCC
KpnI 186 GGTACC GGTAC^C
1670 GGTACC
MfeI 642 CAATTG C^AATTG
1014 CAATTG
MunI 642 CAATTG C^AATTG
1014 CAATTG
NsiI 1199 ATGCAT ATGCA^T
3735 ATGCAT
PpuMI 1552 GGGACCT [GA]G^G[AT]CC[CT]
3763 GGGTCCT
PstI 3428 CTGCAG CTGCA^G
4055 CTGCAG
PvuII 3319 CAGCTG CAG^CTG
3606 CAGCTG
RcaI 692 TCATGA T^CATGA
2728 TCATGA
XbaI 2260 TCTAGA T^CTAGA
2369 TCTAGA
XcmI 782 CCAGCTCCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2855 CCAGGAGCCTGTTGG
XmnI 3417 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3646 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AspHI AvaII BalI
BanI BanII BbrPI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I