##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AviII BbeI
BbrPI BcgI BlpI Bpu1102I BsaAI
BsaHI BsiEI BsiWI BspLU11I BsrFI
Bst1107I BstAPI BstZ17I CelII Cfr10I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI Hin1I KasI
KspI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1723 GGTACC G^GTACC
AccI 1766 GTCTAC GT^[AC][GT]AC
AccIII 3033 TCCGGA T^CCGGA
AseI 4678 ATTAAT AT^TAAT
AsnI 4678 ATTAAT AT^TAAT
Asp718I 1723 GGTACC G^GTACC
AvaI 1134 CTCGGG C^[CT]CG[GA]G
BanII 812 GAGCCC G[GA]GC[CT]^C
BciVI 228 GGATAC GTATCC(6/5)
BclI 2248 TGATCA T^GATCA
BglI 1499 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4904 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3033 TCCGGA T^CCGGA
BsmBI 2916 GAGACG CGTCTC(1/5)
BsmI 1375 GAATGC GAATGC(1/-1)
BsoBI 1134 CTCGGG C^[CT]CG[GA]G
BspEI 3033 TCCGGA T^CCGGA
BsrBI 1543 GAGCGG CCGCTC(-3/-3)
BsrDI 4385 CATTGC GCAATG(2/0)
BssHII 1142 GCGCGC G^CGCGC
BstXI 4359 CCACTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 905 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3702 GACCATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3275 CCATGG C^C[GA][CT]GG
EcoNI 2650 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 365 GAATTC G^AATTC
EcoRV 2217 GATATC GAT^ATC
EcoT22I 3765 ATGCAT ATGCA^T
FbaI 2248 TGATCA T^GATCA
HpaI 2957 GTTAAC GTT^AAC
KpnI 1723 GGTACC GGTAC^C
MamI 4904 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1407 CAATTG C^AATTG
MroI 3033 TCCGGA T^CCGGA
MunI 1407 CAATTG C^AATTG
NcoI 3275 CCATGG C^CATGG
NdeI 3762 CATATG CA^TATG
NsiI 3765 ATGCAT ATGCA^T
PflMI 77 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 417 GTTTAAAC GTTT^AAAC
PpuMI 2084 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1522 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4678 ATTAAT AT^TAAT
SapI 809 GAAGAGC GCTCTTC(1/4)
SexAI 3159 ACCAGGT A^CC[AT]GGT
SfiI 1498 GGCCAGTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 4224 AATATT AAT^ATT
Van91I 77 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 260 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3632 GAATGACTTC
BamHI 2761 GGATCC G^GATCC
3036 GGATCC
BbvCI 272 GCTGAGG CCTCAGC(-5/-2)
3103 GCTGAGG
BpmI 2654 CTGGAG CTGGAG(16/14)
4362 CTGGAG
BspDI 1475 ATCGAT AT^CGAT
2214 ATCGAT
BspHI 1050 TCATGA T^CATGA
1608 TCATGA
BstBI 1089 TTCGAA TT^CGAA
4757 TTCGAA
ClaI 1475 ATCGAT AT^CGAT
2214 ATCGAT
HaeII 1448 AGCGCC [GA]GCGC^[CT]
4334 GGCGCT
HincII 681 GTCAAC GT[CT]^[GA]AC
2957 GTTAAC
HindII 681 GTCAAC GT[CT]^[GA]AC
2957 GTTAAC
HindIII 1461 AAGCTT A^AGCTT
3735 AAGCTT
NspV 1089 TTCGAA TT^CGAA
4757 TTCGAA
PstI 1070 CTGCAG CTGCA^G
1110 CTGCAG
RcaI 1050 TCATGA T^CATGA
1608 TCATGA
ScaI 1386 AGTACT AGT^ACT
3169 AGTACT
SfuI 1089 TTCGAA TT^CGAA
4757 TTCGAA
XcmI 4733 CCAGCTGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4740 CCAGGTGGGTTTTGG
XmnI 260 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3632 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII AvrII BalI BanI
BbsI BbvI BcnI BfaI BfrI
BglII BlnI BmyI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII