################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AviII BbeI BbrPI BcgI BlpI Bpu1102I BsaAI BsaHI BsiEI BsiWI BspLU11I BsrFI Bst1107I BstAPI BstZ17I CelII Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI Hin1I KasI KspI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1723 GGTACC G^GTACC AccI 1766 GTCTAC GT^[AC][GT]AC AccIII 3033 TCCGGA T^CCGGA AseI 4678 ATTAAT AT^TAAT AsnI 4678 ATTAAT AT^TAAT Asp718I 1723 GGTACC G^GTACC AvaI 1134 CTCGGG C^[CT]CG[GA]G BanII 812 GAGCCC G[GA]GC[CT]^C BciVI 228 GGATAC GTATCC(6/5) BclI 2248 TGATCA T^GATCA BglI 1499 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4904 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3033 TCCGGA T^CCGGA BsmBI 2916 GAGACG CGTCTC(1/5) BsmI 1375 GAATGC GAATGC(1/-1) BsoBI 1134 CTCGGG C^[CT]CG[GA]G BspEI 3033 TCCGGA T^CCGGA BsrBI 1543 GAGCGG CCGCTC(-3/-3) BsrDI 4385 CATTGC GCAATG(2/0) BssHII 1142 GCGCGC G^CGCGC BstXI 4359 CCACTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 905 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3702 GACCATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 3275 CCATGG C^C[GA][CT]GG EcoNI 2650 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 365 GAATTC G^AATTC EcoRV 2217 GATATC GAT^ATC EcoT22I 3765 ATGCAT ATGCA^T FbaI 2248 TGATCA T^GATCA HpaI 2957 GTTAAC GTT^AAC KpnI 1723 GGTACC GGTAC^C MamI 4904 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1407 CAATTG C^AATTG MroI 3033 TCCGGA T^CCGGA MunI 1407 CAATTG C^AATTG NcoI 3275 CCATGG C^CATGG NdeI 3762 CATATG CA^TATG NsiI 3765 ATGCAT ATGCA^T PflMI 77 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 417 GTTTAAAC GTTT^AAAC PpuMI 2084 GGGACCT [GA]G^G[AT]CC[CT] PshAI 1522 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4678 ATTAAT AT^TAAT SapI 809 GAAGAGC GCTCTTC(1/4) SexAI 3159 ACCAGGT A^CC[AT]GGT SfiI 1498 GGCCAGTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 4224 AATATT AAT^ATT Van91I 77 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Asp700I 260 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3632 GAATGACTTC BamHI 2761 GGATCC G^GATCC 3036 GGATCC BbvCI 272 GCTGAGG CCTCAGC(-5/-2) 3103 GCTGAGG BpmI 2654 CTGGAG CTGGAG(16/14) 4362 CTGGAG BspDI 1475 ATCGAT AT^CGAT 2214 ATCGAT BspHI 1050 TCATGA T^CATGA 1608 TCATGA BstBI 1089 TTCGAA TT^CGAA 4757 TTCGAA ClaI 1475 ATCGAT AT^CGAT 2214 ATCGAT HaeII 1448 AGCGCC [GA]GCGC^[CT] 4334 GGCGCT HincII 681 GTCAAC GT[CT]^[GA]AC 2957 GTTAAC HindII 681 GTCAAC GT[CT]^[GA]AC 2957 GTTAAC HindIII 1461 AAGCTT A^AGCTT 3735 AAGCTT NspV 1089 TTCGAA TT^CGAA 4757 TTCGAA PstI 1070 CTGCAG CTGCA^G 1110 CTGCAG RcaI 1050 TCATGA T^CATGA 1608 TCATGA ScaI 1386 AGTACT AGT^ACT 3169 AGTACT SfuI 1089 TTCGAA TT^CGAA 4757 TTCGAA XcmI 4733 CCAGCTGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4740 CCAGGTGGGTTTTGG XmnI 260 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3632 GAATGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaII AvrII BalI BanI BbsI BbvI BcnI BfaI BfrI BglII BlnI BmyI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII