##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AflII Alw44I Aor51HI
ApaLI AscI AseI AsnI AviII
AvrII BbeI BcgI BfrI BlnI
BsiEI BsiWI BsmBI BspDI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
HpaI KasI KspI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 4013 GGTACC G^GTACC
AccIII 2412 TCCGGA T^CCGGA
AcyI 3978 GACGCC G[GA]^CG[CT]C
AgeI 4104 ACCGGT A^CCGGT
AhdI 1317 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2992 GGGCCC GGGCC^C
Asp700I 1225 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4013 GGTACC G^GTACC
AspEI 1317 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 443 CACGTG CAC^GTG
BclI 2145 TGATCA T^GATCA
BsaAI 443 CACGTG [CT]AC^GT[GA]
BsaHI 3978 GACGCC G[GA]^CG[CT]C
BseAI 2412 TCCGGA T^CCGGA
BsgI 3683 CTGCAC GTGCAG(16/14)
BsmI 3421 GAATGC GAATGC(1/-1)
BspEI 2412 TCCGGA T^CCGGA
Bst1107I 2471 GTATAC GTA^TAC
BstZ17I 2471 GTATAC GTA^TAC
Eam1105I 1317 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4040 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2164 GAATTC G^AATTC
EcoRV 1847 GATATC GAT^ATC
FbaI 2145 TGATCA T^GATCA
Hin1I 3978 GACGCC G[GA]^CG[CT]C
HincII 1356 GTCGAC GT[CT]^[GA]AC
HindII 1356 GTCGAC GT[CT]^[GA]AC
HindIII 2857 AAGCTT A^AGCTT
KpnI 4013 GGTACC GGTAC^C
MfeI 1993 CAATTG C^AATTG
MroI 2412 TCCGGA T^CCGGA
MunI 1993 CAATTG C^AATTG
NheI 3146 GCTAGC G^CTAGC
PflMI 3341 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4104 ACCGGT A^CCGGT
PmaCI 443 CACGTG CAC^GTG
PmlI 443 CACGTG CAC^GTG
SalI 1356 GTCGAC G^TCGAC
SphI 873 GCATGC GCATG^C
SspI 2273 AATATT AAT^ATT
Van91I 3341 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 141 CCAGTCTCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1225 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1356 GTCGAC GT^[AC][GT]AC
2471 GTATAC
AocI 3153 CCTGAGG CC^T[ACGT]AGG
3806 CCTCAGG
AvaI 577 CTCGGG C^[CT]CG[GA]G
3814 CTCGGG
BalI 1286 TGGCCA TGG^CCA
1907 TGGCCA
BamHI 811 GGATCC G^GATCC
3880 GGATCC
BanI 3435 GGTGCC G^G[CT][GA]CC
4013 GGTACC
BciVI 1737 GGATAC GTATCC(6/5)
759 GTATCC
BcnI 1006 CCGGG CC^[GC]GG
3792 CCCGG
BglI 1001 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2237 GCCGAGCAGGC
BlpI 385 GCTTAGC GC^T[ACGT]AGC
1880 GCTGAGC
Bpu1102I 385 GCTTAGC GC^T[ACGT]AGC
1880 GCTGAGC
BsaBI 1124 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1445 GATGCACATC
BsaI 2399 GAGACC GGTCTC(1/5)
3991 GGTCTC
BsoBI 577 CTCGGG C^[CT]CG[GA]G
3814 CTCGGG
Bsp1407I 597 TGTACA T^GTACA
4093 TGTACA
BspHI 2311 TCATGA T^CATGA
2815 TCATGA
BspLU11I 122 ACATGT A^CATGT
4090 ACATGT
BsrDI 539 CATTGC GCAATG(2/0)
2625 GCAATG
BsrFI 2784 GCCGGT [GA]^CCGG[CT]
4104 ACCGGT
BsrGI 597 TGTACA T^GTACA
4093 TGTACA
BstAPI 853 GCAAGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3512 GCACACCTTGC
Bsu36I 3153 CCTGAGG CC^T[ACGT]AGG
3806 CCTCAGG
CelII 385 GCTTAGC GC^T[ACGT]AGC
1880 GCTGAGC
Cfr10I 2784 GCCGGT [GA]^CCGG[CT]
4104 ACCGGT
DraI 326 TTTAAA TTT^AAA
4189 TTTAAA
DrdI 1675 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4060 GACTCATAGGTC
Eco81I 3153 CCTGAGG CC^T[ACGT]AGG
3806 CCTCAGG
HaeII 581 GGCGCT [GA]GCGC^[CT]
3149 AGCGCC
HgaI 3978 GACGC GACGC(5/10)
3679 GCGTC
MamI 1124 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1445 GATGCACATC
MluNI 1286 TGGCCA TGG^CCA
1907 TGGCCA
MscI 1286 TGGCCA TGG^CCA
1907 TGGCCA
MslI 496 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3291 CATGGGGGTG
NciI 1006 CCGGG CC^[GC]GG
3792 CCCGG
PstI 2901 CTGCAG CTGCA^G
2909 CTGCAG
RcaI 2311 TCATGA T^CATGA
2815 TCATGA
SacI 2315 GAGCTC GAGCT^C
3084 GAGCTC
SapI 821 GAAGAGC GCTCTTC(1/4)
3743 GCTCTTC
SspBI 597 TGTACA T^GTACA
4093 TGTACA
StuI 715 AGGCCT AGG^CCT
3641 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaII BanII BbsI BbvCI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XhoII