##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AscI Asp718I AviII AvrII BamHI
BanI BbeI BbrPI BciVI BfrI
BlnI BsaAI BsiWI BspHI BspLU11I
BsrBI BssHII CpoI DraI DraIII
EcoRV FspI HpaI KasI KpnI
KspI MfeI MluI MunI NarI
NdeI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 3469 TCCGGA T^CCGGA
Alw44I 1017 GTGCAC G^TGCAC
AocI 992 CCTAAGG CC^T[ACGT]AGG
ApaI 3028 GGGCCC GGGCC^C
ApaLI 1017 GTGCAC G^TGCAC
AseI 2943 ATTAAT AT^TAAT
AsnI 2943 ATTAAT AT^TAAT
BglI 1053 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3698 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3698 GCTAAGC GC^T[ACGT]AGC
BseAI 3469 TCCGGA T^CCGGA
BsiEI 12 CGGCCG CG[GA][CT]^CG
BsmI 1405 GCATTC GAATGC(1/-1)
BspDI 2745 ATCGAT AT^CGAT
BspEI 3469 TCCGGA T^CCGGA
BsrDI 2654 CATTGC GCAATG(2/0)
BstBI 3889 TTCGAA TT^CGAA
BstEII 3626 GGTCACC G^GT[ACGT]ACC
BstPI 3626 GGTCACC G^GT[ACGT]ACC
Bsu36I 992 CCTAAGG CC^T[ACGT]AGG
CelII 3698 GCTAAGC GC^T[ACGT]AGC
ClaI 2745 ATCGAT AT^CGAT
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
Eco81I 992 CCTAAGG CC^T[ACGT]AGG
EcoO65I 3626 GGTCACC G^GT[ACGT]ACC
EcoRI 270 GAATTC G^AATTC
EcoT22I 4141 ATGCAT ATGCA^T
FseI 9 GGCCGGCC GGCCGG^CC
HincII 2697 GTTGAC GT[CT]^[GA]AC
HindII 2697 GTTGAC GT[CT]^[GA]AC
MroI 3469 TCCGGA T^CCGGA
NheI 2409 GCTAGC G^CTAGC
NsiI 4141 ATGCAT ATGCA^T
NspV 3889 TTCGAA TT^CGAA
PshBI 2943 ATTAAT AT^TAAT
SacI 1029 GAGCTC GAGCT^C
SapI 912 GAAGAGC GCTCTTC(1/4)
ScaI 2314 AGTACT AGT^ACT
SfuI 3889 TTCGAA TT^CGAA
SgrAI 3786 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 769 CCCGGG CCC^GGG
SspI 4276 AATATT AAT^ATT
XmaCI 769 CCCGGG C^CCGGG
XmaI 769 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AatII 474 GACGTC GACGT^C
1217 GACGTC
AccII 1 CGCG CG^CG
2859 CGCG
AcyI 474 GACGTC G[GA]^CG[CT]C
1217 GACGTC
AhdI 2536 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2949 GACCTGCTGTC
Aor51HI 1263 AGCGCT AGC^GCT
3962 AGCGCT
Asp700I 1374 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3179 GAACGTCTTC
AspEI 2536 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2949 GACCTGCTGTC
AspI 1901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3408 GACGTGGTC
AvaI 769 CCCGGG C^[CT]CG[GA]G
3775 CCCGAG
BbvCI 2281 CCTCAGC CCTCAGC(-5/-2)
386 GCTGAGG
BcgI 2584 CGAGTGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1687 GCAGGCAGATCG
BglII 801 AGATCT A^GATCT
3986 AGATCT
BsaHI 474 GACGTC G[GA]^CG[CT]C
1217 GACGTC
BsmBI 303 CGTCTC CGTCTC(1/5)
1215 GAGACG
BsoBI 769 CCCGGG C^[CT]CG[GA]G
3775 CCCGAG
BspMI 2950 ACCTGC ACCTGC(4/8)
3524 GCAGGT
BsrFI 10 GCCGGC [GA]^CCGG[CT]
3787 GCCGGC
BssSI 2196 CACGAG CACGAG(-5/-1)
4206 CTCGTG
BstAPI 1850 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1859 GCAGGCTGTGC
BstUI 1 CGCG CG^CG
2859 CGCG
BstXI 1323 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1987 CCAGAAGAGTGG
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
3787 GCCGGC
Eam1105I 2536 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2949 GACCTGCTGTC
Eco47III 1263 AGCGCT AGC^GCT
3962 AGCGCT
FauI 881 GCGGG CCCGC(4/6)
1613 GCGGG
HgaI 2406 GACGC GACGC(5/10)
302 GCGTC
Hin1I 474 GACGTC G[GA]^CG[CT]C
1217 GACGTC
HindIII 493 AAGCTT A^AGCTT
1774 AAGCTT
MvnI 1 CGCG CG^CG
2859 CGCG
NaeI 10 GCCGGC GCC^GGC
3787 GCCGGC
NgoMIV 10 GCCGGC G^CCGGC
3787 GCCGGC
PflFI 1901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3408 GACGTGGTC
PstI 3134 CTGCAG CTGCA^G
3455 CTGCAG
SphI 1754 GCATGC GCATG^C
2125 GCATGC
StuI 989 AGGCCT AGG^CCT
1010 AGGCCT
Tth111I 1901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3408 GACGTGGTC
XbaI 762 TCTAGA T^CTAGA
792 TCTAGA
XcmI 1492 CCAAGCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2173 CCATCATTATGTTGG
XmnI 1374 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3179 GAACGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI Bst1107I BstF5I BstNI BstYI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII