################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AscI Asp718I AviII AvrII BamHI BanI BbeI BbrPI BciVI BfrI BlnI BsaAI BsiWI BspHI BspLU11I BsrBI BssHII CpoI DraI DraIII EcoRV FspI HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 3469 TCCGGA T^CCGGA Alw44I 1017 GTGCAC G^TGCAC AocI 992 CCTAAGG CC^T[ACGT]AGG ApaI 3028 GGGCCC GGGCC^C ApaLI 1017 GTGCAC G^TGCAC AseI 2943 ATTAAT AT^TAAT AsnI 2943 ATTAAT AT^TAAT BglI 1053 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3698 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3698 GCTAAGC GC^T[ACGT]AGC BseAI 3469 TCCGGA T^CCGGA BsiEI 12 CGGCCG CG[GA][CT]^CG BsmI 1405 GCATTC GAATGC(1/-1) BspDI 2745 ATCGAT AT^CGAT BspEI 3469 TCCGGA T^CCGGA BsrDI 2654 CATTGC GCAATG(2/0) BstBI 3889 TTCGAA TT^CGAA BstEII 3626 GGTCACC G^GT[ACGT]ACC BstPI 3626 GGTCACC G^GT[ACGT]ACC Bsu36I 992 CCTAAGG CC^T[ACGT]AGG CelII 3698 GCTAAGC GC^T[ACGT]AGC ClaI 2745 ATCGAT AT^CGAT EagI 12 CGGCCG C^GGCCG EclXI 12 CGGCCG C^GGCCG Eco52I 12 CGGCCG C^GGCCG Eco81I 992 CCTAAGG CC^T[ACGT]AGG EcoO65I 3626 GGTCACC G^GT[ACGT]ACC EcoRI 270 GAATTC G^AATTC EcoT22I 4141 ATGCAT ATGCA^T FseI 9 GGCCGGCC GGCCGG^CC HincII 2697 GTTGAC GT[CT]^[GA]AC HindII 2697 GTTGAC GT[CT]^[GA]AC MroI 3469 TCCGGA T^CCGGA NheI 2409 GCTAGC G^CTAGC NsiI 4141 ATGCAT ATGCA^T NspV 3889 TTCGAA TT^CGAA PshBI 2943 ATTAAT AT^TAAT SacI 1029 GAGCTC GAGCT^C SapI 912 GAAGAGC GCTCTTC(1/4) ScaI 2314 AGTACT AGT^ACT SfuI 3889 TTCGAA TT^CGAA SgrAI 3786 CGCCGGCG C[GA]^CCGG[CT]G SmaI 769 CCCGGG CCC^GGG SspI 4276 AATATT AAT^ATT XmaCI 769 CCCGGG C^CCGGG XmaI 769 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AatII 474 GACGTC GACGT^C 1217 GACGTC AccII 1 CGCG CG^CG 2859 CGCG AcyI 474 GACGTC G[GA]^CG[CT]C 1217 GACGTC AhdI 2536 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2949 GACCTGCTGTC Aor51HI 1263 AGCGCT AGC^GCT 3962 AGCGCT Asp700I 1374 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3179 GAACGTCTTC AspEI 2536 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2949 GACCTGCTGTC AspI 1901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3408 GACGTGGTC AvaI 769 CCCGGG C^[CT]CG[GA]G 3775 CCCGAG BbvCI 2281 CCTCAGC CCTCAGC(-5/-2) 386 GCTGAGG BcgI 2584 CGAGTGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1687 GCAGGCAGATCG BglII 801 AGATCT A^GATCT 3986 AGATCT BsaHI 474 GACGTC G[GA]^CG[CT]C 1217 GACGTC BsmBI 303 CGTCTC CGTCTC(1/5) 1215 GAGACG BsoBI 769 CCCGGG C^[CT]CG[GA]G 3775 CCCGAG BspMI 2950 ACCTGC ACCTGC(4/8) 3524 GCAGGT BsrFI 10 GCCGGC [GA]^CCGG[CT] 3787 GCCGGC BssSI 2196 CACGAG CACGAG(-5/-1) 4206 CTCGTG BstAPI 1850 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1859 GCAGGCTGTGC BstUI 1 CGCG CG^CG 2859 CGCG BstXI 1323 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1987 CCAGAAGAGTGG Cfr10I 10 GCCGGC [GA]^CCGG[CT] 3787 GCCGGC Eam1105I 2536 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2949 GACCTGCTGTC Eco47III 1263 AGCGCT AGC^GCT 3962 AGCGCT FauI 881 GCGGG CCCGC(4/6) 1613 GCGGG HgaI 2406 GACGC GACGC(5/10) 302 GCGTC Hin1I 474 GACGTC G[GA]^CG[CT]C 1217 GACGTC HindIII 493 AAGCTT A^AGCTT 1774 AAGCTT MvnI 1 CGCG CG^CG 2859 CGCG NaeI 10 GCCGGC GCC^GGC 3787 GCCGGC NgoMIV 10 GCCGGC G^CCGGC 3787 GCCGGC PflFI 1901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3408 GACGTGGTC PstI 3134 CTGCAG CTGCA^G 3455 CTGCAG SphI 1754 GCATGC GCATG^C 2125 GCATGC StuI 989 AGGCCT AGG^CCT 1010 AGGCCT Tth111I 1901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3408 GACGTGGTC XbaI 762 TCTAGA T^CTAGA 792 TCTAGA XcmI 1492 CCAAGCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2173 CCATCATTATGTTGG XmnI 1374 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3179 GAACGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII