##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbrPI
BcgI BfrI BlnI BsaAI BsiWI
BsmBI BsmI BspDI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DrdI Eco47III EcoRV FseI
FspI KpnI MfeI MluI MunI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SfuI
SgrAI SnaBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 160 GACGTC GACGT^C
AccIII 462 TCCGGA T^CCGGA
AhdI 2619 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1163 CCTCAGG CC^T[ACGT]AGG
AspEI 2619 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2217 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 683 GGCGCC GGCGC^C
BbsI 799 GAAGAC GAAGAC(2/6)
BclI 1523 TGATCA T^GATCA
BlpI 3743 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3743 GCTGAGC GC^T[ACGT]AGC
BpuAI 799 GAAGAC GAAGAC(2/6)
BsaWI 462 TCCGGA [AT]^CCGG[AT]
BseAI 462 TCCGGA T^CCGGA
BsiEI 2027 CGGCCG CG[GA][CT]^CG
Bsp1407I 3838 TGTACA T^GTACA
BspEI 462 TCCGGA T^CCGGA
BsrDI 2774 CATTGC GCAATG(2/0)
BsrGI 3838 TGTACA T^GTACA
BstEII 1200 GGTGACC G^GT[ACGT]ACC
BstPI 1200 GGTGACC G^GT[ACGT]ACC
Bsu36I 1163 CCTCAGG CC^T[ACGT]AGG
CelII 3743 GCTGAGC GC^T[ACGT]AGC
EagI 2027 CGGCCG C^GGCCG
Eam1105I 2619 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2027 CGGCCG C^GGCCG
Eco52I 2027 CGGCCG C^GGCCG
Eco81I 1163 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1200 GGTGACC G^GT[ACGT]ACC
EcoRI 3132 GAATTC G^AATTC
EcoT22I 3813 ATGCAT ATGCA^T
FbaI 1523 TGATCA T^GATCA
HaeII 683 GGCGCC [GA]GCGC^[CT]
HincII 2376 GTTAAC GT[CT]^[GA]AC
HindII 2376 GTTAAC GT[CT]^[GA]AC
HindIII 1617 AAGCTT A^AGCTT
HpaI 2376 GTTAAC GTT^AAC
KasI 683 GGCGCC G^GCGCC
KspI 2024 CCGCGG CCGC^GG
MroI 462 TCCGGA T^CCGGA
NaeI 245 GCCGGC GCC^GGC
NarI 683 GGCGCC GG^CGCC
NgoMIV 245 GCCGGC G^CCGGC
NheI 2487 GCTAGC G^CTAGC
NotI 2026 GCGGCCGC GC^GGCCGC
NsiI 3813 ATGCAT ATGCA^T
PflFI 2217 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 962 AGGACCT [GA]G^G[AT]CC[CT]
SacII 2024 CCGCGG CCGC^GG
SapI 3825 GCTCTTC GCTCTTC(1/4)
SbfI 405 CCTGCAGG CCTGCA^GG
ScaI 3425 AGTACT AGT^ACT
SfiI 2703 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 683 GGCGCC GGC^GCC
SmaI 3313 CCCGGG CCC^GGG
SmlI 2225 CTCAAG C^T[CT][GA]AG
SpeI 2121 ACTAGT A^CTAGT
Sse8387I 405 CCTGCAGG CCTGCA^GG
SspBI 3838 TGTACA T^GTACA
SspI 179 AATATT AAT^ATT
Tth111I 2217 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3313 CCCGGG C^CCGGG
XmaI 3313 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2025 CGCG CG^CG
2258 CGCG
AcsI 3132 GAATTC [GA]^AATT[CT]
4115 AAATTC
ApaI 1647 GGGCCC GGGCC^C
3660 GGGCCC
ApoI 3132 GAATTC [GA]^AATT[CT]
4115 AAATTC
Asp700I 466 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1362 GAACAGCTTC
AvaI 1196 CTCGGG C^[CT]CG[GA]G
3313 CCCGGG
BamHI 2591 GGATCC G^GATCC
3367 GGATCC
BciVI 3971 GGATAC GTATCC(6/5)
942 GTATCC
BglII 1138 AGATCT A^GATCT
1511 AGATCT
BsaBI 111 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1139 GATCTCGATC
BsoBI 1196 CTCGGG C^[CT]CG[GA]G
3313 CCCGGG
BsrBI 780 CCGCTC CCGCTC(-3/-3)
2346 CCGCTC
BssSI 2392 CACGAG CACGAG(-5/-1)
3355 CACGAG
BstUI 2025 CGCG CG^CG
2258 CGCG
BstXI 1112 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2865 CCAGGGAGCTGG
MaeII 161 ACGT A^CGT
1925 ACGT
MamI 111 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1139 GATCTCGATC
MvnI 2025 CGCG CG^CG
2258 CGCG
NcoI 1058 CCATGG C^CATGG
2652 CCATGG
NdeI 321 CATATG CA^TATG
714 CATATG
SexAI 188 ACCTGGT A^CC[AT]GGT
230 ACCTGGT
SphI 827 GCATGC GCATG^C
3815 GCATGC
StuI 1167 AGGCCT AGG^CCT
2702 AGGCCT
XmnI 466 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1362 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII