##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI BcgI
BciVI BglI BsaBI BsiEI BsiWI
Bsp1407I BsrGI BssHII BssSI BstBI
BstEII BstPI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI MamI MluI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1065 TCCGGA T^CCGGA
AcyI 157 GGCGCC G[GA]^CG[CT]C
AflII 3208 CTTAAG C^TTAAG
Alw44I 2957 GTGCAC G^TGCAC
ApaI 2369 GGGCCC GGGCC^C
ApaLI 2957 GTGCAC G^TGCAC
Asp700I 1198 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1187 CCCGAG C^[CT]CG[GA]G
AviII 4459 TGCGCA TGC^GCA
BbeI 157 GGCGCC GGCGC^C
BbrPI 302 CACGTG CAC^GTG
BfrI 3208 CTTAAG C^TTAAG
BsaAI 302 CACGTG [CT]AC^GT[GA]
BsaHI 157 GGCGCC G[GA]^CG[CT]C
BseAI 1065 TCCGGA T^CCGGA
BsmBI 836 GAGACG CGTCTC(1/5)
BsoBI 1187 CCCGAG C^[CT]CG[GA]G
BspDI 2912 ATCGAT AT^CGAT
BspEI 1065 TCCGGA T^CCGGA
BspHI 3088 TCATGA T^CATGA
BspLU11I 3446 ACATGT A^CATGT
Bst1107I 3238 GTATAC GTA^TAC
BstAPI 1476 GCATCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3238 GTATAC GTA^TAC
ClaI 2912 ATCGAT AT^CGAT
DrdI 1922 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 125 GAATTC G^AATTC
EcoRV 4347 GATATC GAT^ATC
FspI 4459 TGCGCA TGC^GCA
HaeII 157 GGCGCC [GA]GCGC^[CT]
Hin1I 157 GGCGCC G[GA]^CG[CT]C
HpaI 4664 GTTAAC GTT^AAC
KasI 157 GGCGCC G^GCGCC
KspI 1737 CCGCGG CCGC^GG
MfeI 3942 CAATTG C^AATTG
MroI 1065 TCCGGA T^CCGGA
MunI 3942 CAATTG C^AATTG
NarI 157 GGCGCC GG^CGCC
NcoI 2733 CCATGG C^CATGG
NheI 3039 GCTAGC G^CTAGC
PflMI 328 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 302 CACGTG CAC^GTG
PmlI 302 CACGTG CAC^GTG
RcaI 3088 TCATGA T^CATGA
SacII 1737 CCGCGG CCGC^GG
SapI 1051 GAAGAGC GCTCTTC(1/4)
SfoI 157 GGCGCC GGC^GCC
SspI 4187 AATATT AAT^ATT
StuI 2133 AGGCCT AGG^CCT
Van91I 328 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2542 TCTAGA T^CTAGA
XmnI 1198 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 3030 GGTACC G^GTACC
4082 GGTACC
AocI 1001 CCTCAGG CC^T[ACGT]AGG
3854 CCTGAGG
Asp718I 3030 GGTACC G^GTACC
4082 GGTACC
AvrII 2762 CCTAGG C^CTAGG
4180 CCTAGG
BalI 1842 TGGCCA TGG^CCA
2322 TGGCCA
BbsI 1177 GAAGAC GAAGAC(2/6)
1247 GAAGAC
BbvCI 2615 CCTCAGC CCTCAGC(-5/-2)
4479 GCTGAGG
BclI 759 TGATCA T^GATCA
2865 TGATCA
BlnI 2762 CCTAGG C^CTAGG
4180 CCTAGG
BpuAI 1177 GAAGAC GAAGAC(2/6)
1247 GAAGAC
BsmI 1588 GAATGC GAATGC(1/-1)
4507 GAATGC
BsrFI 1137 ACCGGC [GA]^CCGG[CT]
2262 ACCGGC
Bsu36I 1001 CCTCAGG CC^T[ACGT]AGG
3854 CCTGAGG
Cfr10I 1137 ACCGGC [GA]^CCGG[CT]
2262 ACCGGC
Eco81I 1001 CCTCAGG CC^T[ACGT]AGG
3854 CCTGAGG
FbaI 759 TGATCA T^GATCA
2865 TGATCA
HgaI 838 GACGC GACGC(5/10)
4382 GCGTC
KpnI 3030 GGTACC GGTAC^C
4082 GGTACC
MluNI 1842 TGGCCA TGG^CCA
2322 TGGCCA
MscI 1842 TGGCCA TGG^CCA
2322 TGGCCA
MslI 2909 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4215 CACTATTGTG
ScaI 765 AGTACT AGT^ACT
4696 AGTACT
SexAI 3645 ACCAGGT A^CC[AT]GGT
4085 ACCTGGT
XcmI 1433 CCAGATCCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3523 CCATCTGTGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaII BamHI BanI
BanII BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII