################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI BcgI BciVI BglI BsaBI BsiEI BsiWI Bsp1407I BsrGI BssHII BssSI BstBI BstEII BstPI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI MamI MluI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 1065 TCCGGA T^CCGGA AcyI 157 GGCGCC G[GA]^CG[CT]C AflII 3208 CTTAAG C^TTAAG Alw44I 2957 GTGCAC G^TGCAC ApaI 2369 GGGCCC GGGCC^C ApaLI 2957 GTGCAC G^TGCAC Asp700I 1198 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1187 CCCGAG C^[CT]CG[GA]G AviII 4459 TGCGCA TGC^GCA BbeI 157 GGCGCC GGCGC^C BbrPI 302 CACGTG CAC^GTG BfrI 3208 CTTAAG C^TTAAG BsaAI 302 CACGTG [CT]AC^GT[GA] BsaHI 157 GGCGCC G[GA]^CG[CT]C BseAI 1065 TCCGGA T^CCGGA BsmBI 836 GAGACG CGTCTC(1/5) BsoBI 1187 CCCGAG C^[CT]CG[GA]G BspDI 2912 ATCGAT AT^CGAT BspEI 1065 TCCGGA T^CCGGA BspHI 3088 TCATGA T^CATGA BspLU11I 3446 ACATGT A^CATGT Bst1107I 3238 GTATAC GTA^TAC BstAPI 1476 GCATCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3238 GTATAC GTA^TAC ClaI 2912 ATCGAT AT^CGAT DrdI 1922 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 125 GAATTC G^AATTC EcoRV 4347 GATATC GAT^ATC FspI 4459 TGCGCA TGC^GCA HaeII 157 GGCGCC [GA]GCGC^[CT] Hin1I 157 GGCGCC G[GA]^CG[CT]C HpaI 4664 GTTAAC GTT^AAC KasI 157 GGCGCC G^GCGCC KspI 1737 CCGCGG CCGC^GG MfeI 3942 CAATTG C^AATTG MroI 1065 TCCGGA T^CCGGA MunI 3942 CAATTG C^AATTG NarI 157 GGCGCC GG^CGCC NcoI 2733 CCATGG C^CATGG NheI 3039 GCTAGC G^CTAGC PflMI 328 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 302 CACGTG CAC^GTG PmlI 302 CACGTG CAC^GTG RcaI 3088 TCATGA T^CATGA SacII 1737 CCGCGG CCGC^GG SapI 1051 GAAGAGC GCTCTTC(1/4) SfoI 157 GGCGCC GGC^GCC SspI 4187 AATATT AAT^ATT StuI 2133 AGGCCT AGG^CCT Van91I 328 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2542 TCTAGA T^CTAGA XmnI 1198 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 3030 GGTACC G^GTACC 4082 GGTACC AocI 1001 CCTCAGG CC^T[ACGT]AGG 3854 CCTGAGG Asp718I 3030 GGTACC G^GTACC 4082 GGTACC AvrII 2762 CCTAGG C^CTAGG 4180 CCTAGG BalI 1842 TGGCCA TGG^CCA 2322 TGGCCA BbsI 1177 GAAGAC GAAGAC(2/6) 1247 GAAGAC BbvCI 2615 CCTCAGC CCTCAGC(-5/-2) 4479 GCTGAGG BclI 759 TGATCA T^GATCA 2865 TGATCA BlnI 2762 CCTAGG C^CTAGG 4180 CCTAGG BpuAI 1177 GAAGAC GAAGAC(2/6) 1247 GAAGAC BsmI 1588 GAATGC GAATGC(1/-1) 4507 GAATGC BsrFI 1137 ACCGGC [GA]^CCGG[CT] 2262 ACCGGC Bsu36I 1001 CCTCAGG CC^T[ACGT]AGG 3854 CCTGAGG Cfr10I 1137 ACCGGC [GA]^CCGG[CT] 2262 ACCGGC Eco81I 1001 CCTCAGG CC^T[ACGT]AGG 3854 CCTGAGG FbaI 759 TGATCA T^GATCA 2865 TGATCA HgaI 838 GACGC GACGC(5/10) 4382 GCGTC KpnI 3030 GGTACC GGTAC^C 4082 GGTACC MluNI 1842 TGGCCA TGG^CCA 2322 TGGCCA MscI 1842 TGGCCA TGG^CCA 2322 TGGCCA MslI 2909 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4215 CACTATTGTG ScaI 765 AGTACT AGT^ACT 4696 AGTACT SexAI 3645 ACCAGGT A^CC[AT]GGT 4085 ACCTGGT XcmI 1433 CCAGATCCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3523 CCATCTGTGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaII BamHI BanI BanII BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII