##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AseI AsnI AviII BbrPI
BciVI BclI BsaAI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BstBI Cfr10I
ClaI CpoI EagI EclXI Eco52I
EcoRV FbaI FseI FspI HpaI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfuI SgrAI SmaI SnaBI
SpeI SphI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2864 GGTACC G^GTACC
AcyI 35 GGCGCC G[GA]^CG[CT]C
AflIII 2683 ACGTGT A^C[GA][CT]GT
AhdI 4716 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5389 GTGCAC G^TGCAC
AocI 801 CCTTAGG CC^T[ACGT]AGG
Aor51HI 271 AGCGCT AGC^GCT
ApaLI 5389 GTGCAC G^TGCAC
Asp700I 3273 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2864 GGTACC G^GTACC
AspEI 4716 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 317 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1046 GGATCC G^GATCC
BbeI 35 GGCGCC GGCGC^C
BglII 2004 AGATCT A^GATCT
BlpI 349 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 349 GCTCAGC GC^T[ACGT]AGC
BsaHI 35 GGCGCC G[GA]^CG[CT]C
Bsp1407I 2553 TGTACA T^GTACA
BsrGI 2553 TGTACA T^GTACA
Bst1107I 4584 GTATAC GTA^TAC
BstEII 5072 GGTTACC G^GT[ACGT]ACC
BstPI 5072 GGTTACC G^GT[ACGT]ACC
BstZ17I 4584 GTATAC GTA^TAC
Bsu36I 801 CCTTAGG CC^T[ACGT]AGG
CelII 349 GCTCAGC GC^T[ACGT]AGC
DrdI 1308 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4716 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 271 AGCGCT AGC^GCT
Eco81I 801 CCTTAGG CC^T[ACGT]AGG
EcoO65I 5072 GGTTACC G^GT[ACGT]ACC
EcoT22I 2126 ATGCAT ATGCA^T
HgaI 5430 GACGC GACGC(5/10)
Hin1I 35 GGCGCC G[GA]^CG[CT]C
KasI 35 GGCGCC G^GCGCC
KpnI 2864 GGTACC GGTAC^C
MaeII 2683 ACGT A^CGT
NarI 35 GGCGCC GG^CGCC
NcoI 864 CCATGG C^CATGG
NsiI 2126 ATGCAT ATGCA^T
PflFI 317 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4284 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 619 CCTGCAGG CCTGCA^GG
SexAI 3374 ACCTGGT A^CC[AT]GGT
SfiI 5415 GGCCAAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 35 GGCGCC GGC^GCC
Sse8387I 619 CCTGCAGG CCTGCA^GG
SspBI 2553 TGTACA T^GTACA
StuI 793 AGGCCT AGG^CCT
Tth111I 317 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4284 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3273 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 60 CGCG CG^CG
375 CGCG
AflII 5315 CTTAAG C^TTAAG
5398 CTTAAG
BcgI 5128 CGACAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2505 GCATAGGATTCG
BfrI 5315 CTTAAG C^TTAAG
5398 CTTAAG
BpmI 3263 CTCCAG CTGGAG(16/14)
4626 CTCCAG
BsaBI 142 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4298 GATAATCATC
BsaWI 3078 ACCGGA [AT]^CCGG[AT]
4068 ACCGGA
BspHI 2398 TCATGA T^CATGA
5538 TCATGA
BsrDI 811 CATTGC GCAATG(2/0)
3791 GCAATG
BssSI 365 CACGAG CACGAG(-5/-1)
1270 CACGAG
BstAPI 3668 GCACCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3752 GCACAGGGTGC
BstUI 60 CGCG CG^CG
375 CGCG
DraI 3764 TTTAAA TTT^AAA
4872 TTTAAA
EcoRI 3633 GAATTC G^AATTC
4381 GAATTC
HaeII 35 GGCGCC [GA]GCGC^[CT]
271 AGCGCT
KspI 59 CCGCGG CCGC^GG
374 CCGCGG
MamI 142 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4298 GATAATCATC
MvnI 60 CGCG CG^CG
375 CGCG
NheI 3909 GCTAGC G^CTAGC
5195 GCTAGC
NspI 3728 ACATGC [GA]CATG^[CT]
4478 GCATGT
PaeR7I 560 CTCGAG C^TCGAG
1665 CTCGAG
PpuMI 603 AGGACCT [GA]G^G[AT]CC[CT]
2767 GGGACCC
RcaI 2398 TCATGA T^CATGA
5538 TCATGA
SacII 59 CCGCGG CCGC^GG
374 CCGCGG
SapI 1780 GAAGAGC GCTCTTC(1/4)
3238 GCTCTTC
ScaI 846 AGTACT AGT^ACT
2598 AGTACT
SspI 981 AATATT AAT^ATT
3480 AATATT
XhoI 560 CTCGAG C^TCGAG
1665 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BmyI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII