################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AclI AgeI ApaI AscI AseI AsnI AviII BbrPI BciVI BclI BsaAI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BstBI Cfr10I ClaI CpoI EagI EclXI Eco52I EcoRV FbaI FseI FspI HpaI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 2864 GGTACC G^GTACC AcyI 35 GGCGCC G[GA]^CG[CT]C AflIII 2683 ACGTGT A^C[GA][CT]GT AhdI 4716 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5389 GTGCAC G^TGCAC AocI 801 CCTTAGG CC^T[ACGT]AGG Aor51HI 271 AGCGCT AGC^GCT ApaLI 5389 GTGCAC G^TGCAC Asp700I 3273 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2864 GGTACC G^GTACC AspEI 4716 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 317 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1046 GGATCC G^GATCC BbeI 35 GGCGCC GGCGC^C BglII 2004 AGATCT A^GATCT BlpI 349 GCTCAGC GC^T[ACGT]AGC Bpu1102I 349 GCTCAGC GC^T[ACGT]AGC BsaHI 35 GGCGCC G[GA]^CG[CT]C Bsp1407I 2553 TGTACA T^GTACA BsrGI 2553 TGTACA T^GTACA Bst1107I 4584 GTATAC GTA^TAC BstEII 5072 GGTTACC G^GT[ACGT]ACC BstPI 5072 GGTTACC G^GT[ACGT]ACC BstZ17I 4584 GTATAC GTA^TAC Bsu36I 801 CCTTAGG CC^T[ACGT]AGG CelII 349 GCTCAGC GC^T[ACGT]AGC DrdI 1308 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 4716 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 271 AGCGCT AGC^GCT Eco81I 801 CCTTAGG CC^T[ACGT]AGG EcoO65I 5072 GGTTACC G^GT[ACGT]ACC EcoT22I 2126 ATGCAT ATGCA^T HgaI 5430 GACGC GACGC(5/10) Hin1I 35 GGCGCC G[GA]^CG[CT]C KasI 35 GGCGCC G^GCGCC KpnI 2864 GGTACC GGTAC^C MaeII 2683 ACGT A^CGT NarI 35 GGCGCC GG^CGCC NcoI 864 CCATGG C^CATGG NsiI 2126 ATGCAT ATGCA^T PflFI 317 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4284 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SbfI 619 CCTGCAGG CCTGCA^GG SexAI 3374 ACCTGGT A^CC[AT]GGT SfiI 5415 GGCCAAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 35 GGCGCC GGC^GCC Sse8387I 619 CCTGCAGG CCTGCA^GG SspBI 2553 TGTACA T^GTACA StuI 793 AGGCCT AGG^CCT Tth111I 317 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4284 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3273 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 60 CGCG CG^CG 375 CGCG AflII 5315 CTTAAG C^TTAAG 5398 CTTAAG BcgI 5128 CGACAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2505 GCATAGGATTCG BfrI 5315 CTTAAG C^TTAAG 5398 CTTAAG BpmI 3263 CTCCAG CTGGAG(16/14) 4626 CTCCAG BsaBI 142 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4298 GATAATCATC BsaWI 3078 ACCGGA [AT]^CCGG[AT] 4068 ACCGGA BspHI 2398 TCATGA T^CATGA 5538 TCATGA BsrDI 811 CATTGC GCAATG(2/0) 3791 GCAATG BssSI 365 CACGAG CACGAG(-5/-1) 1270 CACGAG BstAPI 3668 GCACCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3752 GCACAGGGTGC BstUI 60 CGCG CG^CG 375 CGCG DraI 3764 TTTAAA TTT^AAA 4872 TTTAAA EcoRI 3633 GAATTC G^AATTC 4381 GAATTC HaeII 35 GGCGCC [GA]GCGC^[CT] 271 AGCGCT KspI 59 CCGCGG CCGC^GG 374 CCGCGG MamI 142 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4298 GATAATCATC MvnI 60 CGCG CG^CG 375 CGCG NheI 3909 GCTAGC G^CTAGC 5195 GCTAGC NspI 3728 ACATGC [GA]CATG^[CT] 4478 GCATGT PaeR7I 560 CTCGAG C^TCGAG 1665 CTCGAG PpuMI 603 AGGACCT [GA]G^G[AT]CC[CT] 2767 GGGACCC RcaI 2398 TCATGA T^CATGA 5538 TCATGA SacII 59 CCGCGG CCGC^GG 374 CCGCGG SapI 1780 GAAGAGC GCTCTTC(1/4) 3238 GCTCTTC ScaI 846 AGTACT AGT^ACT 2598 AGTACT SspI 981 AATATT AAT^ATT 3480 AATATT XhoI 560 CTCGAG C^TCGAG 1665 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BmyI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII