################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AspI AviII AvrII BamHI BclI BlnI BlpI Bpu1102I BsaBI BsaI BsiWI BsmI BspDI BspHI BssHII CelII ClaI CpoI Eco47III EcoRI FbaI FseI FspI HpaI MamI MluI NcoI NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 433 GACGTC GACGT^C AflII 3524 CTTAAG C^TTAAG AhdI 462 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 2702 GGGCCC GGGCC^C Asp700I 3293 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 462 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2798 GGCGCC GGCGC^C BcgI 2341 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3524 CTTAAG C^TTAAG BglII 3834 AGATCT A^GATCT BsiEI 1077 CGGCCG CG[GA][CT]^CG Bsp1407I 2588 TGTACA T^GTACA BspLU11I 787 ACATGT A^CATGT BsrDI 3194 GCAATG GCAATG(2/0) BsrGI 2588 TGTACA T^GTACA BssSI 386 CACGAG CACGAG(-5/-1) Bst1107I 3814 GTATAC GTA^TAC BstAPI 1095 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 496 TTCGAA TT^CGAA BstEII 4080 GGTGACC G^GT[ACGT]ACC BstPI 4080 GGTGACC G^GT[ACGT]ACC BstXI 2174 CCATGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 3834 AGATCT [GA]^GATC[CT] BstZ17I 3814 GTATAC GTA^TAC DrdI 3901 GACAGGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1077 CGGCCG C^GGCCG Eam1105I 462 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1077 CGGCCG C^GGCCG Eco52I 1077 CGGCCG C^GGCCG EcoNI 818 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4080 GGTGACC G^GT[ACGT]ACC EcoT22I 3622 ATGCAT ATGCA^T HincII 2564 GTCGAC GT[CT]^[GA]AC HindII 2564 GTCGAC GT[CT]^[GA]AC KasI 2798 GGCGCC G^GCGCC KspI 75 CCGCGG CCGC^GG MflI 3834 AGATCT [GA]^GATC[CT] NaeI 1075 GCCGGC GCC^GGC NarI 2798 GGCGCC GG^CGCC NdeI 3715 CATATG CA^TATG NgoMIV 1075 GCCGGC G^CCGGC NsiI 3622 ATGCAT ATGCA^T NspV 496 TTCGAA TT^CGAA PshAI 3496 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 75 CCGCGG CCGC^GG SalI 2564 GTCGAC G^TCGAC SapI 1265 GCTCTTC GCTCTTC(1/4) ScaI 449 AGTACT AGT^ACT SfoI 2798 GGCGCC GGC^GCC SfuI 496 TTCGAA TT^CGAA SphI 1082 GCATGC GCATG^C SspBI 2588 TGTACA T^GTACA XbaI 4174 TCTAGA T^CTAGA XhoII 3834 AGATCT [GA]^GATC[CT] XmnI 3293 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 159 TCCGGA T^CCGGA 1456 TCCGGA AocI 2149 CCTCAGG CC^T[ACGT]AGG 3302 CCTTAGG BalI 2032 TGGCCA TGG^CCA 3280 TGGCCA BbrPI 84 CACGTG CAC^GTG 1637 CACGTG BsaAI 84 CACGTG [CT]AC^GT[GA] 1637 CACGTG BsaWI 159 TCCGGA [AT]^CCGG[AT] 1456 TCCGGA BseAI 159 TCCGGA T^CCGGA 1456 TCCGGA BspEI 159 TCCGGA T^CCGGA 1456 TCCGGA Bsu36I 2149 CCTCAGG CC^T[ACGT]AGG 3302 CCTTAGG DraIII 1096 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1810 CACATGGTG Eco81I 2149 CCTCAGG CC^T[ACGT]AGG 3302 CCTTAGG EcoRV 1595 GATATC GAT^ATC 4316 GATATC HaeII 70 AGCGCC [GA]GCGC^[CT] 2798 GGCGCC HgaI 744 GACGC GACGC(5/10) 1153 GCGTC HindIII 212 AAGCTT A^AGCTT 3451 AAGCTT MfeI 532 CAATTG C^AATTG 3636 CAATTG MluNI 2032 TGGCCA TGG^CCA 3280 TGGCCA MroI 159 TCCGGA T^CCGGA 1456 TCCGGA MscI 2032 TGGCCA TGG^CCA 3280 TGGCCA MunI 532 CAATTG C^AATTG 3636 CAATTG PmaCI 84 CACGTG CAC^GTG 1637 CACGTG PmlI 84 CACGTG CAC^GTG 1637 CACGTG SacI 720 GAGCTC GAGCT^C 3945 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmaCI XmaI