##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AclI        AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspI        AviII       
AvrII       BamHI       BclI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BsiWI       BsmI        
BspDI       BspHI       BssHII      CelII       ClaI        
CpoI        Eco47III    EcoRI       FbaI        FseI        
FspI        HpaI        MamI        MluI        NcoI        
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SbfI        
SexAI       SfiI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tth111I     Van91I      
XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII         433  GACGTC               GACGT^C
AflII        3524  CTTAAG               C^TTAAG
AhdI          462  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         2702  GGGCCC               GGGCC^C
Asp700I      3293  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         462  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         2798  GGCGCC               GGCGC^C
BcgI         2341  CGACCTCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3524  CTTAAG               C^TTAAG
BglII        3834  AGATCT               A^GATCT
BsiEI        1077  CGGCCG               CG[GA][CT]^CG
Bsp1407I     2588  TGTACA               T^GTACA
BspLU11I      787  ACATGT               A^CATGT
BsrDI        3194  GCAATG               GCAATG(2/0)
BsrGI        2588  TGTACA               T^GTACA
BssSI         386  CACGAG               CACGAG(-5/-1)
Bst1107I     3814  GTATAC               GTA^TAC
BstAPI       1095  GCACCAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         496  TTCGAA               TT^CGAA
BstEII       4080  GGTGACC              G^GT[ACGT]ACC
BstPI        4080  GGTGACC              G^GT[ACGT]ACC
BstXI        2174  CCATGCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        3834  AGATCT               [GA]^GATC[CT]
BstZ17I      3814  GTATAC               GTA^TAC
DrdI         3901  GACAGGTGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1077  CGGCCG               C^GGCCG
Eam1105I      462  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1077  CGGCCG               C^GGCCG
Eco52I       1077  CGGCCG               C^GGCCG
EcoNI         818  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      4080  GGTGACC              G^GT[ACGT]ACC
EcoT22I      3622  ATGCAT               ATGCA^T
HincII       2564  GTCGAC               GT[CT]^[GA]AC
HindII       2564  GTCGAC               GT[CT]^[GA]AC
KasI         2798  GGCGCC               G^GCGCC
KspI           75  CCGCGG               CCGC^GG
MflI         3834  AGATCT               [GA]^GATC[CT]
NaeI         1075  GCCGGC               GCC^GGC
NarI         2798  GGCGCC               GG^CGCC
NdeI         3715  CATATG               CA^TATG
NgoMIV       1075  GCCGGC               G^CCGGC
NsiI         3622  ATGCAT               ATGCA^T
NspV          496  TTCGAA               TT^CGAA
PshAI        3496  GACTGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          75  CCGCGG               CCGC^GG
SalI         2564  GTCGAC               G^TCGAC
SapI         1265  GCTCTTC              GCTCTTC(1/4)
ScaI          449  AGTACT               AGT^ACT
SfoI         2798  GGCGCC               GGC^GCC
SfuI          496  TTCGAA               TT^CGAA
SphI         1082  GCATGC               GCATG^C
SspBI        2588  TGTACA               T^GTACA
XbaI         4174  TCTAGA               T^CTAGA
XhoII        3834  AGATCT               [GA]^GATC[CT]
XmnI         3293  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII        159  TCCGGA               T^CCGGA
             1456  TCCGGA              
AocI         2149  CCTCAGG              CC^T[ACGT]AGG
             3302  CCTTAGG             
BalI         2032  TGGCCA               TGG^CCA
             3280  TGGCCA              
BbrPI          84  CACGTG               CAC^GTG
             1637  CACGTG              
BsaAI          84  CACGTG               [CT]AC^GT[GA]
             1637  CACGTG              
BsaWI         159  TCCGGA               [AT]^CCGG[AT]
             1456  TCCGGA              
BseAI         159  TCCGGA               T^CCGGA
             1456  TCCGGA              
BspEI         159  TCCGGA               T^CCGGA
             1456  TCCGGA              
Bsu36I       2149  CCTCAGG              CC^T[ACGT]AGG
             3302  CCTTAGG             
DraIII       1096  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1810  CACATGGTG           
Eco81I       2149  CCTCAGG              CC^T[ACGT]AGG
             3302  CCTTAGG             
EcoRV        1595  GATATC               GAT^ATC
             4316  GATATC              
HaeII          70  AGCGCC               [GA]GCGC^[CT]
             2798  GGCGCC              
HgaI          744  GACGC                GACGC(5/10)
             1153  GCGTC               
HindIII       212  AAGCTT               A^AGCTT
             3451  AAGCTT              
MfeI          532  CAATTG               C^AATTG
             3636  CAATTG              
MluNI        2032  TGGCCA               TGG^CCA
             3280  TGGCCA              
MroI          159  TCCGGA               T^CCGGA
             1456  TCCGGA              
MscI         2032  TGGCCA               TGG^CCA
             3280  TGGCCA              
MunI          532  CAATTG               C^AATTG
             3636  CAATTG              
PmaCI          84  CACGTG               CAC^GTG
             1637  CACGTG              
PmlI           84  CACGTG               CAC^GTG
             1637  CACGTG              
SacI          720  GAGCTC               GAGCT^C
             3945  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AcyI        AfaI        AflIII      AluI        AlwI        
AlwNI       ApoI        Asp718I     AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvCI       
BbvI        BciVI       BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XmaCI       XmaI