##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AviII
AvrII BamHI BclI BlnI BlpI
Bpu1102I BsaBI BsaI BsiWI BsmI
BspDI BspHI BssHII CelII ClaI
CpoI Eco47III EcoRI FbaI FseI
FspI HpaI MamI MluI NcoI
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SbfI
SexAI SfiI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 433 GACGTC GACGT^C
AflII 3524 CTTAAG C^TTAAG
AhdI 462 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2702 GGGCCC GGGCC^C
Asp700I 3293 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 462 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2798 GGCGCC GGCGC^C
BcgI 2341 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3524 CTTAAG C^TTAAG
BglII 3834 AGATCT A^GATCT
BsiEI 1077 CGGCCG CG[GA][CT]^CG
Bsp1407I 2588 TGTACA T^GTACA
BspLU11I 787 ACATGT A^CATGT
BsrDI 3194 GCAATG GCAATG(2/0)
BsrGI 2588 TGTACA T^GTACA
BssSI 386 CACGAG CACGAG(-5/-1)
Bst1107I 3814 GTATAC GTA^TAC
BstAPI 1095 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 496 TTCGAA TT^CGAA
BstEII 4080 GGTGACC G^GT[ACGT]ACC
BstPI 4080 GGTGACC G^GT[ACGT]ACC
BstXI 2174 CCATGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3834 AGATCT [GA]^GATC[CT]
BstZ17I 3814 GTATAC GTA^TAC
DrdI 3901 GACAGGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1077 CGGCCG C^GGCCG
Eam1105I 462 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1077 CGGCCG C^GGCCG
Eco52I 1077 CGGCCG C^GGCCG
EcoNI 818 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4080 GGTGACC G^GT[ACGT]ACC
EcoT22I 3622 ATGCAT ATGCA^T
HincII 2564 GTCGAC GT[CT]^[GA]AC
HindII 2564 GTCGAC GT[CT]^[GA]AC
KasI 2798 GGCGCC G^GCGCC
KspI 75 CCGCGG CCGC^GG
MflI 3834 AGATCT [GA]^GATC[CT]
NaeI 1075 GCCGGC GCC^GGC
NarI 2798 GGCGCC GG^CGCC
NdeI 3715 CATATG CA^TATG
NgoMIV 1075 GCCGGC G^CCGGC
NsiI 3622 ATGCAT ATGCA^T
NspV 496 TTCGAA TT^CGAA
PshAI 3496 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 75 CCGCGG CCGC^GG
SalI 2564 GTCGAC G^TCGAC
SapI 1265 GCTCTTC GCTCTTC(1/4)
ScaI 449 AGTACT AGT^ACT
SfoI 2798 GGCGCC GGC^GCC
SfuI 496 TTCGAA TT^CGAA
SphI 1082 GCATGC GCATG^C
SspBI 2588 TGTACA T^GTACA
XbaI 4174 TCTAGA T^CTAGA
XhoII 3834 AGATCT [GA]^GATC[CT]
XmnI 3293 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 159 TCCGGA T^CCGGA
1456 TCCGGA
AocI 2149 CCTCAGG CC^T[ACGT]AGG
3302 CCTTAGG
BalI 2032 TGGCCA TGG^CCA
3280 TGGCCA
BbrPI 84 CACGTG CAC^GTG
1637 CACGTG
BsaAI 84 CACGTG [CT]AC^GT[GA]
1637 CACGTG
BsaWI 159 TCCGGA [AT]^CCGG[AT]
1456 TCCGGA
BseAI 159 TCCGGA T^CCGGA
1456 TCCGGA
BspEI 159 TCCGGA T^CCGGA
1456 TCCGGA
Bsu36I 2149 CCTCAGG CC^T[ACGT]AGG
3302 CCTTAGG
DraIII 1096 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1810 CACATGGTG
Eco81I 2149 CCTCAGG CC^T[ACGT]AGG
3302 CCTTAGG
EcoRV 1595 GATATC GAT^ATC
4316 GATATC
HaeII 70 AGCGCC [GA]GCGC^[CT]
2798 GGCGCC
HgaI 744 GACGC GACGC(5/10)
1153 GCGTC
HindIII 212 AAGCTT A^AGCTT
3451 AAGCTT
MfeI 532 CAATTG C^AATTG
3636 CAATTG
MluNI 2032 TGGCCA TGG^CCA
3280 TGGCCA
MroI 159 TCCGGA T^CCGGA
1456 TCCGGA
MscI 2032 TGGCCA TGG^CCA
3280 TGGCCA
MunI 532 CAATTG C^AATTG
3636 CAATTG
PmaCI 84 CACGTG CAC^GTG
1637 CACGTG
PmlI 84 CACGTG CAC^GTG
1637 CACGTG
SacI 720 GAGCTC GAGCT^C
3945 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI ApoI Asp718I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI