##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AscI Asp718I AspEI BamHI BclI
BfrI BglII BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
DraI DrdI Eam1105I EcoRV EcoT22I
FbaI FseI HindIII KpnI MamI
MluI NdeI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PvuI RcaI SbfI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1805 GTCGAC GT^[AC][GT]AC
AclI 3013 AACGTT AA^CGTT
AflIII 3944 ACGTGT A^C[GA][CT]GT
Alw44I 2790 GTGCAC G^TGCAC
AocI 2289 CCTGAGG CC^T[ACGT]AGG
Aor51HI 45 AGCGCT AGC^GCT
ApaLI 2790 GTGCAC G^TGCAC
AseI 3546 ATTAAT AT^TAAT
AsnI 3546 ATTAAT AT^TAAT
Asp700I 2105 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3534 CCTAGG C^CTAGG
BbrPI 3943 CACGTG CAC^GTG
BlnI 3534 CCTAGG C^CTAGG
BlpI 1922 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1922 GCTGAGC GC^T[ACGT]AGC
BsaAI 3943 CACGTG [CT]AC^GT[GA]
BsmBI 51 GAGACG CGTCTC(1/5)
BssSI 250 CTCGTG CACGAG(-5/-1)
BstEII 1562 GGTGACC G^GT[ACGT]ACC
BstPI 1562 GGTGACC G^GT[ACGT]ACC
BstYI 3936 GGATCT [GA]^GATC[CT]
Bsu36I 2289 CCTGAGG CC^T[ACGT]AGG
CelII 1922 GCTGAGC GC^T[ACGT]AGC
CpoI 2711 CGGTCCG CG^G[AT]CCG
DraIII 425 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 45 AGCGCT AGC^GCT
Eco81I 2289 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1562 GGTGACC G^GT[ACGT]ACC
EcoRI 1059 GAATTC G^AATTC
HpaI 4051 GTTAAC GTT^AAC
MfeI 4102 CAATTG C^AATTG
MflI 3936 GGATCT [GA]^GATC[CT]
MunI 4102 CAATTG C^AATTG
NcoI 2722 CCATGG C^CATGG
NheI 2358 GCTAGC G^CTAGC
NotI 1320 GCGGCCGC GC^GGCCGC
PmaCI 3943 CACGTG CAC^GTG
PmlI 3943 CACGTG CAC^GTG
PshBI 3546 ATTAAT AT^TAAT
Psp1406I 3013 AACGTT AA^CGTT
RsrII 2711 CGGTCCG CG^G[AT]CCG
SacI 594 GAGCTC GAGCT^C
SalI 1805 GTCGAC G^TCGAC
SapI 639 GCTCTTC GCTCTTC(1/4)
SphI 979 GCATGC GCATG^C
SspI 3474 AATATT AAT^ATT
XcmI 538 CCAAGAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3936 GGATCT [GA]^GATC[CT]
XmnI 2105 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 61 TCCGGA T^CCGGA
2960 TCCGGA
AspI 1799 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2070 GACGCGGTC
AviII 1936 TGCGCA TGC^GCA
2476 TGCGCA
BalI 2164 TGGCCA TGG^CCA
2974 TGGCCA
BbsI 946 GAAGAC GAAGAC(2/6)
3739 GTCTTC
BpuAI 946 GAAGAC GAAGAC(2/6)
3739 GTCTTC
BsaI 3235 GAGACC GGTCTC(1/5)
2075 GGTCTC
BseAI 61 TCCGGA T^CCGGA
2960 TCCGGA
BsgI 2006 CTGCAC GTGCAG(16/14)
2461 GTGCAG
BspEI 61 TCCGGA T^CCGGA
2960 TCCGGA
BsrBI 1786 CCGCTC CCGCTC(-3/-3)
108 GAGCGG
BsrDI 2137 GCAATG GCAATG(2/0)
2878 GCAATG
EcoNI 773 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
851 CCTCCCGTAGG
FspI 1936 TGCGCA TGC^GCA
2476 TGCGCA
HincII 1805 GTCGAC GT[CT]^[GA]AC
4051 GTTAAC
HindII 1805 GTCGAC GT[CT]^[GA]AC
4051 GTTAAC
MluNI 2164 TGGCCA TGG^CCA
2974 TGGCCA
MroI 61 TCCGGA T^CCGGA
2960 TCCGGA
MscI 2164 TGGCCA TGG^CCA
2974 TGGCCA
PflFI 1799 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2070 GACGCGGTC
PstI 153 CTGCAG CTGCA^G
1270 CTGCAG
SfiI 271 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3098 GGCCCAGGGGGCC
StuI 1845 AGGCCT AGG^CCT
1909 AGGCCT
TfiI 393 GAATC G^A[AT]TC
2964 GATTC
Tsp45I 1563 GTGAC ^GT[GC]AC
2837 GTCAC
Tth111I 1799 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2070 GACGCGGTC
XbaI 3081 TCTAGA T^CTAGA
3292 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI