##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
AlwI Aor51HI AscI AseI AsnI
AspEI AviII BamHI BbeI BbvCI
BcgI BglI BglII BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BstEII BstPI
BstYI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HpaI KasI KspI MamI MfeI
MflI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SmaI
SnaBI SphI Sse8387I SwaI XbaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1502 GGTACC G^GTACC
AclI 701 AACGTT AA^CGTT
Asp700I 304 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1502 GGTACC G^GTACC
AspI 2896 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4283 CACGTG CAC^GTG
BbsI 4148 GAAGAC GAAGAC(2/6)
BciVI 1118 GTATCC GTATCC(6/5)
BclI 4245 TGATCA T^GATCA
BpuAI 4148 GAAGAC GAAGAC(2/6)
BsaI 3677 GAGACC GGTCTC(1/5)
Bsp1407I 4097 TGTACA T^GTACA
BspLU11I 3429 ACATGT A^CATGT
BspMI 3791 ACCTGC ACCTGC(4/8)
BsrDI 2180 GCAATG GCAATG(2/0)
BsrGI 4097 TGTACA T^GTACA
Bst1107I 557 GTATAC GTA^TAC
BstZ17I 557 GTATAC GTA^TAC
DraI 2982 TTTAAA TTT^AAA
DrdI 1828 GACTTCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4201 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 4245 TGATCA T^GATCA
HaeII 1011 AGCGCC [GA]GCGC^[CT]
HindIII 1314 AAGCTT A^AGCTT
KpnI 1502 GGTACC GGTAC^C
NcoI 535 CCATGG C^CATGG
PflFI 2896 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4283 CACGTG CAC^GTG
PmlI 4283 CACGTG CAC^GTG
Psp1406I 701 AACGTT AA^CGTT
SacI 1676 GAGCTC GAGCT^C
SapI 1678 GCTCTTC GCTCTTC(1/4)
SgrAI 54 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3228 ACTAGT A^CTAGT
SspBI 4097 TGTACA T^GTACA
SspI 2846 AATATT AAT^ATT
StuI 939 AGGCCT AGG^CCT
Tth111I 2896 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 304 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 557 GTATAC GT^[AC][GT]AC
3904 GTCTAC
AflIII 3429 ACATGT A^C[GA][CT]GT
4282 ACACGT
Alw44I 2770 GTGCAC G^TGCAC
3594 GTGCAC
ApaLI 2770 GTGCAC G^TGCAC
3594 GTGCAC
AvrII 266 CCTAGG C^CTAGG
3918 CCTAGG
BanI 1502 GGTACC G^G[CT][GA]CC
1963 GGCACC
BlnI 266 CCTAGG C^CTAGG
3918 CCTAGG
BsaAI 3591 TACGTG [CT]AC^GT[GA]
4283 CACGTG
BspHI 504 TCATGA T^CATGA
4248 TCATGA
BsrBI 605 GAGCGG CCGCTC(-3/-3)
749 GAGCGG
BsrFI 55 GCCGGC [GA]^CCGG[CT]
913 GCCGGC
BssSI 1113 CACGAG CACGAG(-5/-1)
1795 CACGAG
BstBI 1471 TTCGAA TT^CGAA
1734 TTCGAA
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
913 GCCGGC
DpnII 502 GATC ^GATC
4246 GATC
DraIII 1965 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2571 CACATTGTG
MboI 502 GATC ^GATC
4246 GATC
NaeI 55 GCCGGC GCC^GGC
913 GCCGGC
NdeII 502 GATC ^GATC
4246 GATC
NgoMIV 55 GCCGGC G^CCGGC
913 GCCGGC
NspV 1471 TTCGAA TT^CGAA
1734 TTCGAA
PvuII 1512 CAGCTG CAG^CTG
1752 CAGCTG
RcaI 504 TCATGA T^CATGA
4248 TCATGA
Sau3AI 502 GATC ^GATC
4246 GATC
SexAI 1966 ACCAGGT A^CC[AT]GGT
2263 ACCAGGT
SfuI 1471 TTCGAA TT^CGAA
1734 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BfaI BfrI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI