##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp718I
BbeI BbvCI BcgI BciVI BclI
BfrI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
HaeII HpaI KasI KpnI MamI
MluI MroI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PstI
RcaI RsrII SalI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflIII 1083 ACACGT A^C[GA][CT]GT
AgeI 775 ACCGGT A^CCGGT
AhdI 1675 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2144 CCTCAGG CC^T[ACGT]AGG
Asp700I 1462 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1675 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2965 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 650 CCTAGG C^CTAGG
BamHI 2080 GGATCC G^GATCC
BbrPI 981 CACGTG CAC^GTG
BlnI 650 CCTAGG C^CTAGG
BlpI 143 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 143 GCTGAGC GC^T[ACGT]AGC
BsmBI 502 GAGACG CGTCTC(1/5)
BsmI 3227 GAATGC GAATGC(1/-1)
BssSI 2607 CTCGTG CACGAG(-5/-1)
Bsu36I 2144 CCTCAGG CC^T[ACGT]AGG
CelII 143 GCTGAGC GC^T[ACGT]AGC
DraI 3410 TTTAAA TTT^AAA
DraIII 636 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1675 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2144 CCTCAGG CC^T[ACGT]AGG
HincII 1268 GTCAAC GT[CT]^[GA]AC
HindII 1268 GTCAAC GT[CT]^[GA]AC
KspI 204 CCGCGG CCGC^GG
MfeI 2339 CAATTG C^AATTG
MunI 2339 CAATTG C^AATTG
PaeR7I 249 CTCGAG C^TCGAG
PflFI 2965 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3050 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 775 ACCGGT A^CCGGT
PmaCI 981 CACGTG CAC^GTG
PmlI 981 CACGTG CAC^GTG
PpuMI 1808 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 77 CGATCG CGAT^CG
PvuII 3002 CAGCTG CAG^CTG
SacII 204 CCGCGG CCGC^GG
ScaI 657 AGTACT AGT^ACT
SexAI 637 ACCTGGT A^CC[AT]GGT
SfiI 42 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2928 CCCGGG CCC^GGG
Tth111I 2965 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3050 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 249 CTCGAG C^TCGAG
XmaCI 2928 CCCGGG C^CCGGG
XmaI 2928 CCCGGG C^CCGGG
XmnI 1462 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 771 GTAGAC GT^[AC][GT]AC
2512 GTCTAC
AcyI 757 GACGCC G[GA]^CG[CT]C
868 GACGCC
Alw44I 596 GTGCAC G^TGCAC
707 GTGCAC
AlwNI 1522 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
2703 CAGCATCTG
ApaLI 596 GTGCAC G^TGCAC
707 GTGCAC
AviII 693 TGCGCA TGC^GCA
1113 TGCGCA
BalI 804 TGGCCA TGG^CCA
1699 TGGCCA
BsaHI 757 GACGCC G[GA]^CG[CT]C
868 GACGCC
BsrBI 4 CCGCTC CCGCTC(-3/-3)
3524 CCGCTC
BsrDI 452 GCAATG GCAATG(2/0)
2306 GCAATG
BstAPI 2542 GCACATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2998 GCATCAGCTGC
FseI 47 GGCCGGCC GGCCGG^CC
176 GGCCGGCC
FspI 693 TGCGCA TGC^GCA
1113 TGCGCA
Hin1I 757 GACGCC G[GA]^CG[CT]C
868 GACGCC
HindIII 1775 AAGCTT A^AGCTT
2586 AAGCTT
MluNI 804 TGGCCA TGG^CCA
1699 TGGCCA
MscI 804 TGGCCA TGG^CCA
1699 TGGCCA
NaeI 48 GCCGGC GCC^GGC
177 GCCGGC
NgoMIV 48 GCCGGC G^CCGGC
177 GCCGGC
SacI 1097 GAGCTC GAGCT^C
2409 GAGCTC
SapI 1999 GCTCTTC GCTCTTC(1/4)
2411 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII