##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccI AccIII AflII Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AviII AvrII BbeI BbrPI
BcgI BfrI BglII BlnI BsaAI
BseAI BsiEI BsiWI BspEI BspMI
BssHII Bst1107I BstXI BstZ17I Bsu36I
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRV FseI FspI
HgaI HindIII HpaI KasI KspI
MfeI MluI MroI MunI NarI
NdeI NotI PacI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 4859 AACGTT AA^CGTT
AgeI 690 ACCGGT A^CCGGT
AhdI 4440 GACTAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2183 GGGCCC GGGCC^C
AspEI 4440 GACTAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4404 GGATCC G^GATCC
BglI 3875 GCCTACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 859 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2783 GAATGC GAATGC(1/-1)
Bsp1407I 3003 TGTACA T^GTACA
BspDI 2366 ATCGAT AT^CGAT
BspHI 1284 TCATGA T^CATGA
BsrGI 3003 TGTACA T^GTACA
BstAPI 2695 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4433 TTCGAA TT^CGAA
BstEII 3607 GGTCACC G^GT[ACGT]ACC
BstPI 3607 GGTCACC G^GT[ACGT]ACC
ClaI 2366 ATCGAT AT^CGAT
Eam1105I 4440 GACTAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3607 GGTCACC G^GT[ACGT]ACC
EcoRI 1316 GAATTC G^AATTC
EcoT22I 860 ATGCAT ATGCA^T
HaeII 3395 AGCGCC [GA]GCGC^[CT]
HincII 377 GTCAAC GT[CT]^[GA]AC
HindII 377 GTCAAC GT[CT]^[GA]AC
MamI 859 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2653 CCATGG C^CATGG
NheI 3233 GCTAGC G^CTAGC
NruI 1041 TCGCGA TCG^CGA
NsiI 860 ATGCAT ATGCA^T
NspV 4433 TTCGAA TT^CGAA
PaeR7I 1854 CTCGAG C^TCGAG
PflMI 2299 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 690 ACCGGT A^CCGGT
Psp1406I 4859 AACGTT AA^CGTT
RcaI 1284 TCATGA T^CATGA
SfuI 4433 TTCGAA TT^CGAA
SpeI 373 ACTAGT A^CTAGT
SspBI 3003 TGTACA T^GTACA
SspI 4134 AATATT AAT^ATT
StuI 1749 AGGCCT AGG^CCT
Van91I 2299 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1854 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 2221 GACGTC GACGT^C
2353 GACGTC
AcyI 2221 GACGTC G[GA]^CG[CT]C
2353 GACGTC
AspI 1859 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3446 GACACAGTC
BbvCI 3651 CCTCAGC CCTCAGC(-5/-2)
4974 CCTCAGC
BciVI 304 GGATAC GTATCC(6/5)
3974 GTATCC
BlpI 2402 GCTGAGC GC^T[ACGT]AGC
3391 GCTGAGC
Bpu1102I 2402 GCTGAGC GC^T[ACGT]AGC
3391 GCTGAGC
BsaHI 2221 GACGTC G[GA]^CG[CT]C
2353 GACGTC
BsaI 3926 GAGACC GGTCTC(1/5)
4780 GAGACC
BsaWI 498 TCCGGT [AT]^CCGG[AT]
690 ACCGGT
BspLU11I 2791 ACATGT A^CATGT
3009 ACATGT
BsrBI 1591 CCGCTC CCGCTC(-3/-3)
3561 CCGCTC
CelII 2402 GCTGAGC GC^T[ACGT]AGC
3391 GCTGAGC
DsaI 2196 CCGTGG C^C[GA][CT]GG
2653 CCATGG
Hin1I 2221 GACGTC G[GA]^CG[CT]C
2353 GACGTC
NaeI 3115 GCCGGC GCC^GGC
3398 GCCGGC
NgoMIV 3115 GCCGGC G^CCGGC
3398 GCCGGC
PflFI 1859 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3446 GACACAGTC
SacI 1463 GAGCTC GAGCT^C
2987 GAGCTC
SapI 766 GAAGAGC GCTCTTC(1/4)
3172 GCTCTTC
ScaI 2757 AGTACT AGT^ACT
4113 AGTACT
SmaI 1419 CCCGGG CCC^GGG
1511 CCCGGG
Tth111I 1859 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3446 GACACAGTC
XmaCI 1419 CCCGGG C^CCGGG
1511 CCCGGG
XmaI 1419 CCCGGG C^CCGGG
1511 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII