##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvaI AviII BbrPI BcgI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiWI BsoBI Bsp1407I BspDI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI Eco47III EcoNI
EcoO65I EcoRI EcoRV FseI FspI
KpnI MamI MfeI MluI MunI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 3202 GTAGAC GT^[AC][GT]AC
AccIII 158 TCCGGA T^CCGGA
AhdI 2673 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3901 CCTTAGG CC^T[ACGT]AGG
AspEI 2673 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4439 CCTAGG C^CTAGG
BalI 4266 TGGCCA TGG^CCA
BamHI 3321 GGATCC G^GATCC
BanI 201 GGCGCC G^G[CT][GA]CC
BbeI 201 GGCGCC GGCGC^C
BciVI 2357 GGATAC GTATCC(6/5)
BglII 4179 AGATCT A^GATCT
BlnI 4439 CCTAGG C^CTAGG
BseAI 158 TCCGGA T^CCGGA
BsgI 1268 GTGCAG GTGCAG(16/14)
BsiEI 179 CGGCCG CG[GA][CT]^CG
BsmBI 1368 CGTCTC CGTCTC(1/5)
BsmI 1032 GAATGC GAATGC(1/-1)
BspEI 158 TCCGGA T^CCGGA
BspMI 1714 GCAGGT ACCTGC(4/8)
BsrFI 217 GCCGGC [GA]^CCGG[CT]
BstXI 2234 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3901 CCTTAGG CC^T[ACGT]AGG
Cfr10I 217 GCCGGC [GA]^CCGG[CT]
DraIII 3119 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4120 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 179 CGGCCG C^GGCCG
Eam1105I 2673 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 179 CGGCCG C^GGCCG
Eco52I 179 CGGCCG C^GGCCG
Eco81I 3901 CCTTAGG CC^T[ACGT]AGG
EcoT22I 3337 ATGCAT ATGCA^T
HaeII 201 GGCGCC [GA]GCGC^[CT]
HpaI 4329 GTTAAC GTT^AAC
KasI 201 GGCGCC G^GCGCC
KspI 192 CCGCGG CCGC^GG
MaeII 435 ACGT A^CGT
MluNI 4266 TGGCCA TGG^CCA
MroI 158 TCCGGA T^CCGGA
MscI 4266 TGGCCA TGG^CCA
MspA1I 192 CCGCGG C[AC]G^C[GT]G
NaeI 217 GCCGGC GCC^GGC
NarI 201 GGCGCC GG^CGCC
NdeI 4843 CATATG CA^TATG
NgoMIV 217 GCCGGC G^CCGGC
NheI 3727 GCTAGC G^CTAGC
NsiI 3337 ATGCAT ATGCA^T
PpuMI 3660 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 192 CCGCGG CCGC^GG
SfcI 759 CTACAG C^T[GA][CT]AG
SfoI 201 GGCGCC GGC^GCC
SpeI 3770 ACTAGT A^CTAGT
StuI 3001 AGGCCT AGG^CCT
SwaI 4046 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 42 GACGCC G[GA]^CG[CT]C
201 GGCGCC
AflIII 873 ACATGT A^C[GA][CT]GT
4575 ACATGT
AlwNI 88 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
2773 CAGAGCCTG
AseI 4089 ATTAAT AT^TAAT
4553 ATTAAT
AsnI 4089 ATTAAT AT^TAAT
4553 ATTAAT
AspHI 412 GAGCTC G[AT]GC[AT]^C
1000 GAGCTC
BbsI 1440 GTCTTC GAAGAC(2/6)
3106 GTCTTC
BbvCI 1535 CCTCAGC CCTCAGC(-5/-2)
2555 CCTCAGC
BclI 731 TGATCA T^GATCA
4677 TGATCA
BglI 51 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
164 GCCTGCTGGGC
BpmI 1761 CTCCAG CTGGAG(16/14)
2078 CTCCAG
BpuAI 1440 GTCTTC GAAGAC(2/6)
3106 GTCTTC
BsaHI 42 GACGCC G[GA]^CG[CT]C
201 GGCGCC
BsaWI 158 TCCGGA [AT]^CCGG[AT]
404 ACCGGA
BsiHKAI 412 GAGCTC G[AT]GC[AT]^C
1000 GAGCTC
BspLU11I 873 ACATGT A^CATGT
4575 ACATGT
BstAPI 952 GCATTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1159 GCAGAGGATGC
DraII 1634 GGGGCCT [GA]G^G[ACGT]CC[CT]
3660 GGGTCCC
EaeI 179 CGGCCG [CT]^GGCC[GA]
4266 TGGCCA
EcoO109I 1634 GGGGCCT [GA]G^G[ACGT]CC[CT]
3660 GGGTCCC
FbaI 731 TGATCA T^GATCA
4677 TGATCA
HgaI 42 GACGC GACGC(5/10)
310 GACGC
Hin1I 42 GACGCC G[GA]^CG[CT]C
201 GGCGCC
HincII 238 GTTGAC GT[CT]^[GA]AC
4329 GTTAAC
HindII 238 GTTGAC GT[CT]^[GA]AC
4329 GTTAAC
HindIII 667 AAGCTT A^AGCTT
3290 AAGCTT
PshBI 4089 ATTAAT AT^TAAT
4553 ATTAAT
SacI 412 GAGCTC GAGCT^C
1000 GAGCTC
SapI 742 GAAGAGC GCTCTTC(1/4)
414 GCTCTTC
ScaI 1895 AGTACT AGT^ACT
2410 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI Asp700I AvaII
BanII BbvI BcnI BfaI BfrI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI