################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AviII BalI BamHI BbrPI BfrI BglI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FauI FseI FspI HindIII KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NcoI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 848 GGTACC G^GTACC AflIII 782 ACATGT A^C[GA][CT]GT Alw44I 2393 GTGCAC G^TGCAC ApaLI 2393 GTGCAC G^TGCAC Asp718I 848 GGTACC G^GTACC AspI 2775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3418 CCTAGG C^CTAGG BbeI 68 GGCGCC GGCGC^C BcgI 111 CGAGTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2753 GGATAC GTATCC(6/5) BclI 3542 TGATCA T^GATCA BlnI 3418 CCTAGG C^CTAGG BsaAI 258 TACGTG [CT]AC^GT[GA] BsaI 3735 GAGACC GGTCTC(1/5) BspHI 3470 TCATGA T^CATGA BspLU11I 782 ACATGT A^CATGT Bst1107I 680 GTATAC GTA^TAC BstAPI 1794 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 197 CCATTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 680 GTATAC GTA^TAC EcoRI 3921 GAATTC G^AATTC EcoRV 2546 GATATC GAT^ATC FbaI 3542 TGATCA T^GATCA HpaI 3332 GTTAAC GTT^AAC KasI 68 GGCGCC G^GCGCC KpnI 848 GGTACC GGTAC^C NarI 68 GGCGCC GG^CGCC NheI 2522 GCTAGC G^CTAGC PflFI 2775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2026 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1811 CAGCTG CAG^CTG RcaI 3470 TCATGA T^CATGA SfoI 68 GGCGCC GGC^GCC SphI 421 GCATGC GCATG^C SwaI 442 ATTTAAAT ATTT^AAAT Tth111I 2775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 68 GGCGCC G[GA]^CG[CT]C 1720 GGCGTC AocI 1110 CCTAAGG CC^T[ACGT]AGG 4455 CCTTAGG BbvCI 573 CCTCAGC CCTCAGC(-5/-2) 3409 CCTCAGC BlpI 1813 GCTGAGC GC^T[ACGT]AGC 2709 GCTGAGC Bpu1102I 1813 GCTGAGC GC^T[ACGT]AGC 2709 GCTGAGC BsaBI 2530 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2546 GATATCTATC BsaHI 68 GGCGCC G[GA]^CG[CT]C 1720 GGCGTC BsgI 626 GTGCAG GTGCAG(16/14) 2483 GTGCAG BsmI 1138 GAATGC GAATGC(1/-1) 4261 GAATGC BspMI 1466 ACCTGC ACCTGC(4/8) 3150 ACCTGC BsrDI 555 CATTGC GCAATG(2/0) 4551 GCAATG BssSI 2240 CTCGTG CACGAG(-5/-1) 4015 CTCGTG Bsu36I 1110 CCTAAGG CC^T[ACGT]AGG 4455 CCTTAGG CelII 1813 GCTGAGC GC^T[ACGT]AGC 2709 GCTGAGC Eco81I 1110 CCTAAGG CC^T[ACGT]AGG 4455 CCTTAGG EcoT14I 704 CCAAGG C^C[AT][AT]GG 3418 CCTAGG HaeII 68 GGCGCC [GA]GCGC^[CT] 3798 AGCGCC HgaI 4548 GACGC GACGC(5/10) 1721 GCGTC Hin1I 68 GGCGCC G[GA]^CG[CT]C 1720 GGCGTC HincII 3332 GTTAAC GT[CT]^[GA]AC 4243 GTTGAC HindII 3332 GTTAAC GT[CT]^[GA]AC 4243 GTTGAC MamI 2530 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2546 GATATCTATC NdeI 2110 CATATG CA^TATG 2303 CATATG PaeR7I 2347 CTCGAG C^TCGAG 3629 CTCGAG PleI 2081 GACTC GAGTC(4/5) 2488 GACTC PstI 1160 CTGCAG CTGCA^G 4567 CTGCAG SmaI 1282 CCCGGG CCC^GGG 3434 CCCGGG StyI 704 CCAAGG C^C[AT][AT]GG 3418 CCTAGG Tsp45I 2843 GTCAC ^GT[GC]AC 4202 GTGAC XcmI 1753 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3578 CCAAGAAGAATGTGG XhoI 2347 CTCGAG C^TCGAG 3629 CTCGAG XmaCI 1282 CCCGGG C^CCGGG 3434 CCCGGG XmaI 1282 CCCGGG C^CCGGG 3434 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII EarI EcoO109I EcoRII EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PpuMI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI