##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AviII BalI
BamHI BbrPI BfrI BglI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BstBI
BstEII BstPI BstUI Cfr10I ClaI
CpoI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FseI FspI HindIII
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NcoI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 848 GGTACC G^GTACC
AflIII 782 ACATGT A^C[GA][CT]GT
Alw44I 2393 GTGCAC G^TGCAC
ApaLI 2393 GTGCAC G^TGCAC
Asp718I 848 GGTACC G^GTACC
AspI 2775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3418 CCTAGG C^CTAGG
BbeI 68 GGCGCC GGCGC^C
BcgI 111 CGAGTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2753 GGATAC GTATCC(6/5)
BclI 3542 TGATCA T^GATCA
BlnI 3418 CCTAGG C^CTAGG
BsaAI 258 TACGTG [CT]AC^GT[GA]
BsaI 3735 GAGACC GGTCTC(1/5)
BspHI 3470 TCATGA T^CATGA
BspLU11I 782 ACATGT A^CATGT
Bst1107I 680 GTATAC GTA^TAC
BstAPI 1794 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 197 CCATTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 680 GTATAC GTA^TAC
EcoRI 3921 GAATTC G^AATTC
EcoRV 2546 GATATC GAT^ATC
FbaI 3542 TGATCA T^GATCA
HpaI 3332 GTTAAC GTT^AAC
KasI 68 GGCGCC G^GCGCC
KpnI 848 GGTACC GGTAC^C
NarI 68 GGCGCC GG^CGCC
NheI 2522 GCTAGC G^CTAGC
PflFI 2775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2026 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1811 CAGCTG CAG^CTG
RcaI 3470 TCATGA T^CATGA
SfoI 68 GGCGCC GGC^GCC
SphI 421 GCATGC GCATG^C
SwaI 442 ATTTAAAT ATTT^AAAT
Tth111I 2775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 68 GGCGCC G[GA]^CG[CT]C
1720 GGCGTC
AocI 1110 CCTAAGG CC^T[ACGT]AGG
4455 CCTTAGG
BbvCI 573 CCTCAGC CCTCAGC(-5/-2)
3409 CCTCAGC
BlpI 1813 GCTGAGC GC^T[ACGT]AGC
2709 GCTGAGC
Bpu1102I 1813 GCTGAGC GC^T[ACGT]AGC
2709 GCTGAGC
BsaBI 2530 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2546 GATATCTATC
BsaHI 68 GGCGCC G[GA]^CG[CT]C
1720 GGCGTC
BsgI 626 GTGCAG GTGCAG(16/14)
2483 GTGCAG
BsmI 1138 GAATGC GAATGC(1/-1)
4261 GAATGC
BspMI 1466 ACCTGC ACCTGC(4/8)
3150 ACCTGC
BsrDI 555 CATTGC GCAATG(2/0)
4551 GCAATG
BssSI 2240 CTCGTG CACGAG(-5/-1)
4015 CTCGTG
Bsu36I 1110 CCTAAGG CC^T[ACGT]AGG
4455 CCTTAGG
CelII 1813 GCTGAGC GC^T[ACGT]AGC
2709 GCTGAGC
Eco81I 1110 CCTAAGG CC^T[ACGT]AGG
4455 CCTTAGG
EcoT14I 704 CCAAGG C^C[AT][AT]GG
3418 CCTAGG
HaeII 68 GGCGCC [GA]GCGC^[CT]
3798 AGCGCC
HgaI 4548 GACGC GACGC(5/10)
1721 GCGTC
Hin1I 68 GGCGCC G[GA]^CG[CT]C
1720 GGCGTC
HincII 3332 GTTAAC GT[CT]^[GA]AC
4243 GTTGAC
HindII 3332 GTTAAC GT[CT]^[GA]AC
4243 GTTGAC
MamI 2530 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2546 GATATCTATC
NdeI 2110 CATATG CA^TATG
2303 CATATG
PaeR7I 2347 CTCGAG C^TCGAG
3629 CTCGAG
PleI 2081 GACTC GAGTC(4/5)
2488 GACTC
PstI 1160 CTGCAG CTGCA^G
4567 CTGCAG
SmaI 1282 CCCGGG CCC^GGG
3434 CCCGGG
StyI 704 CCAAGG C^C[AT][AT]GG
3418 CCTAGG
Tsp45I 2843 GTCAC ^GT[GC]AC
4202 GTGAC
XcmI 1753 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3578 CCAAGAAGAATGTGG
XhoI 2347 CTCGAG C^TCGAG
3629 CTCGAG
XmaCI 1282 CCCGGG C^CCGGG
3434 CCCGGG
XmaI 1282 CCCGGG C^CCGGG
3434 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EarI EcoO109I EcoRII EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PpuMI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI