################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AvaI AviII BamHI BbeI BfrI BlpI Bpu1102I BsaHI BsiEI BsiWI BsoBI BspDI BspMI BssHII BssSI BstBI BstEII BstPI CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI Hin1I HincII HindII HpaI KasI KspI MluI NarI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 426 CGCG CG^CG AccIII 765 TCCGGA T^CCGGA AhdI 2350 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1670 GGGCCC GGGCC^C AspEI 2350 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 2867 CACGTG CAC^GTG BcgI 2670 GCATTGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 168 TGATCA T^GATCA BcnI 4354 CCGGG CC^[GC]GG BglI 2032 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2867 CACGTG [CT]AC^GT[GA] BsaWI 765 TCCGGA [AT]^CCGG[AT] BseAI 765 TCCGGA T^CCGGA BsgI 2337 CTGCAC GTGCAG(16/14) BspEI 765 TCCGGA T^CCGGA BsrBI 36 CCGCTC CCGCTC(-3/-3) BsrFI 379 GCCGGC [GA]^CCGG[CT] BstAPI 2271 GCATAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 426 CGCG CG^CG Cfr10I 379 GCCGGC [GA]^CCGG[CT] Eam1105I 2350 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1058 GATATC GAT^ATC FbaI 168 TGATCA T^GATCA HaeII 717 AGCGCC [GA]GCGC^[CT] HgaI 1587 GCGTC GACGC(5/10) HindIII 5018 AAGCTT A^AGCTT MroI 765 TCCGGA T^CCGGA MvnI 426 CGCG CG^CG NaeI 379 GCCGGC GCC^GGC NciI 4354 CCGGG CC^[GC]GG NcoI 1340 CCATGG C^CATGG NgoMIV 379 GCCGGC G^CCGGC PflFI 1721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2867 CACGTG CAC^GTG PmlI 2867 CACGTG CAC^GTG PshAI 1483 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 4542 AGTACT AGT^ACT SexAI 1366 ACCTGGT A^CC[AT]GGT SphI 2276 GCATGC GCATG^C Tth111I 1721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 857 GGTACC G^GTACC 4957 GGTACC AcsI 1181 AAATTT [GA]^AATT[CT] 2754 AAATTT AocI 4031 CCTCAGG CC^T[ACGT]AGG 4393 CCTAAGG ApoI 1181 AAATTT [GA]^AATT[CT] 2754 AAATTT Asp718I 857 GGTACC G^GTACC 4957 GGTACC AvrII 2396 CCTAGG C^CTAGG 4818 CCTAGG BalI 840 TGGCCA TGG^CCA 4146 TGGCCA BbsI 2476 GTCTTC GAAGAC(2/6) 3951 GTCTTC BciVI 2012 GGATAC GTATCC(6/5) 4273 GTATCC BglII 471 AGATCT A^GATCT 868 AGATCT BlnI 2396 CCTAGG C^CTAGG 4818 CCTAGG BpuAI 2476 GTCTTC GAAGAC(2/6) 3951 GTCTTC BsaBI 661 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3805 GATTTGAATC BsaI 5 GAGACC GGTCTC(1/5) 3221 GGTCTC BsmBI 722 CGTCTC CGTCTC(1/5) 1612 CGTCTC BspLU11I 60 ACATGT A^CATGT 2747 ACATGT Bst1107I 2489 GTATAC GTA^TAC 2511 GTATAC BstZ17I 2489 GTATAC GTA^TAC 2511 GTATAC Bsu36I 4031 CCTCAGG CC^T[ACGT]AGG 4393 CCTAAGG CfoI 461 GCGC GCG^C 718 GCGC DraIII 2497 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4656 CACCCTGTG DrdI 107 GACCCGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1978 GACAGTTATGTC Eco81I 4031 CCTCAGG CC^T[ACGT]AGG 4393 CCTAAGG EcoNI 4515 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4528 CCTCTCCAAGG HhaI 461 GCGC GCG^C 718 GCGC HinP1I 461 GCGC G^CGC 718 GCGC KpnI 857 GGTACC GGTAC^C 4957 GGTACC MamI 661 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3805 GATTTGAATC MfeI 2731 CAATTG C^AATTG 4942 CAATTG MluNI 840 TGGCCA TGG^CCA 4146 TGGCCA MscI 840 TGGCCA TGG^CCA 4146 TGGCCA MunI 2731 CAATTG C^AATTG 4942 CAATTG NheI 46 GCTAGC G^CTAGC 4484 GCTAGC PstI 1690 CTGCAG CTGCA^G 3890 CTGCAG TaqI 3 TCGA T^CGA 75 TCGA TthHB8I 3 TCGA T^CGA 75 TCGA XbaI 657 TCTAGA T^CTAGA 1523 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AfaI AflIII AluI AlwI AlwNI Asp700I AspHI AvaII BanI BanII BbvCI BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmnI