##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AvaI AviII BamHI BbeI
BfrI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsoBI BspDI BspMI BssHII
BssSI BstBI BstEII BstPI CelII
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI FseI FspI
Hin1I HincII HindII HpaI KasI
KspI MluI NarI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 426 CGCG CG^CG
AccIII 765 TCCGGA T^CCGGA
AhdI 2350 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1670 GGGCCC GGGCC^C
AspEI 2350 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2867 CACGTG CAC^GTG
BcgI 2670 GCATTGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 168 TGATCA T^GATCA
BcnI 4354 CCGGG CC^[GC]GG
BglI 2032 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2867 CACGTG [CT]AC^GT[GA]
BsaWI 765 TCCGGA [AT]^CCGG[AT]
BseAI 765 TCCGGA T^CCGGA
BsgI 2337 CTGCAC GTGCAG(16/14)
BspEI 765 TCCGGA T^CCGGA
BsrBI 36 CCGCTC CCGCTC(-3/-3)
BsrFI 379 GCCGGC [GA]^CCGG[CT]
BstAPI 2271 GCATAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 426 CGCG CG^CG
Cfr10I 379 GCCGGC [GA]^CCGG[CT]
Eam1105I 2350 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1058 GATATC GAT^ATC
FbaI 168 TGATCA T^GATCA
HaeII 717 AGCGCC [GA]GCGC^[CT]
HgaI 1587 GCGTC GACGC(5/10)
HindIII 5018 AAGCTT A^AGCTT
MroI 765 TCCGGA T^CCGGA
MvnI 426 CGCG CG^CG
NaeI 379 GCCGGC GCC^GGC
NciI 4354 CCGGG CC^[GC]GG
NcoI 1340 CCATGG C^CATGG
NgoMIV 379 GCCGGC G^CCGGC
PflFI 1721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2867 CACGTG CAC^GTG
PmlI 2867 CACGTG CAC^GTG
PshAI 1483 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 4542 AGTACT AGT^ACT
SexAI 1366 ACCTGGT A^CC[AT]GGT
SphI 2276 GCATGC GCATG^C
Tth111I 1721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 857 GGTACC G^GTACC
4957 GGTACC
AcsI 1181 AAATTT [GA]^AATT[CT]
2754 AAATTT
AocI 4031 CCTCAGG CC^T[ACGT]AGG
4393 CCTAAGG
ApoI 1181 AAATTT [GA]^AATT[CT]
2754 AAATTT
Asp718I 857 GGTACC G^GTACC
4957 GGTACC
AvrII 2396 CCTAGG C^CTAGG
4818 CCTAGG
BalI 840 TGGCCA TGG^CCA
4146 TGGCCA
BbsI 2476 GTCTTC GAAGAC(2/6)
3951 GTCTTC
BciVI 2012 GGATAC GTATCC(6/5)
4273 GTATCC
BglII 471 AGATCT A^GATCT
868 AGATCT
BlnI 2396 CCTAGG C^CTAGG
4818 CCTAGG
BpuAI 2476 GTCTTC GAAGAC(2/6)
3951 GTCTTC
BsaBI 661 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3805 GATTTGAATC
BsaI 5 GAGACC GGTCTC(1/5)
3221 GGTCTC
BsmBI 722 CGTCTC CGTCTC(1/5)
1612 CGTCTC
BspLU11I 60 ACATGT A^CATGT
2747 ACATGT
Bst1107I 2489 GTATAC GTA^TAC
2511 GTATAC
BstZ17I 2489 GTATAC GTA^TAC
2511 GTATAC
Bsu36I 4031 CCTCAGG CC^T[ACGT]AGG
4393 CCTAAGG
CfoI 461 GCGC GCG^C
718 GCGC
DraIII 2497 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4656 CACCCTGTG
DrdI 107 GACCCGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1978 GACAGTTATGTC
Eco81I 4031 CCTCAGG CC^T[ACGT]AGG
4393 CCTAAGG
EcoNI 4515 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4528 CCTCTCCAAGG
HhaI 461 GCGC GCG^C
718 GCGC
HinP1I 461 GCGC G^CGC
718 GCGC
KpnI 857 GGTACC GGTAC^C
4957 GGTACC
MamI 661 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3805 GATTTGAATC
MfeI 2731 CAATTG C^AATTG
4942 CAATTG
MluNI 840 TGGCCA TGG^CCA
4146 TGGCCA
MscI 840 TGGCCA TGG^CCA
4146 TGGCCA
MunI 2731 CAATTG C^AATTG
4942 CAATTG
NheI 46 GCTAGC G^CTAGC
4484 GCTAGC
PstI 1690 CTGCAG CTGCA^G
3890 CTGCAG
TaqI 3 TCGA T^CGA
75 TCGA
TthHB8I 3 TCGA T^CGA
75 TCGA
XbaI 657 TCTAGA T^CTAGA
1523 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AfaI AflIII AluI
AlwI AlwNI Asp700I AspHI AvaII
BanI BanII BbvCI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmnI