##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AcyI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbeI BbrPI BsaHI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BstAPI BstBI
BstUI CfoI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 198 GGTACC G^GTACC
AclI 3838 AACGTT AA^CGTT
AcsI 1261 AAATTC [GA]^AATT[CT]
AflII 3826 CTTAAG C^TTAAG
AgeI 2952 ACCGGT A^CCGGT
AocI 1648 CCTCAGG CC^T[ACGT]AGG
ApoI 1261 AAATTC [GA]^AATT[CT]
Asp700I 1489 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 198 GGTACC G^GTACC
AspI 932 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1213 CGAGGATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 15 TGATCA T^GATCA
BfrI 3826 CTTAAG C^TTAAG
BglI 1391 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 558 AGATCT A^GATCT
BsaAI 3487 TACGTG [CT]AC^GT[GA]
BsaBI 1292 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2952 ACCGGT [AT]^CCGG[AT]
BsmBI 2136 CGTCTC CGTCTC(1/5)
BsmI 631 GAATGC GAATGC(1/-1)
BspHI 4013 TCATGA T^CATGA
BspMI 3471 ACCTGC ACCTGC(4/8)
BssSI 296 CACGAG CACGAG(-5/-1)
Bst1107I 2836 GTATAC GTA^TAC
BstEII 3160 GGTCACC G^GT[ACGT]ACC
BstPI 3160 GGTCACC G^GT[ACGT]ACC
BstXI 1776 CCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2836 GTATAC GTA^TAC
Bsu36I 1648 CCTCAGG CC^T[ACGT]AGG
Eco81I 1648 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3160 GGTCACC G^GT[ACGT]ACC
FbaI 15 TGATCA T^GATCA
HindIII 1697 AAGCTT A^AGCTT
KpnI 198 GGTACC GGTAC^C
MamI 1292 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 826 GCTAGC G^CTAGC
PaeR7I 359 CTCGAG C^TCGAG
PflFI 932 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2952 ACCGGT A^CCGGT
Psp1406I 3838 AACGTT AA^CGTT
RcaI 4013 TCATGA T^CATGA
SapI 3193 GAAGAGC GCTCTTC(1/4)
SbfI 2540 CCTGCAGG CCTGCA^GG
ScaI 661 AGTACT AGT^ACT
Sse8387I 2540 CCTGCAGG CCTGCA^GG
SspI 2889 AATATT AAT^ATT
Tth111I 932 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 226 TCTAGA T^CTAGA
XhoI 359 CTCGAG C^TCGAG
XmnI 1489 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2836 GTATAC GT^[AC][GT]AC
3042 GTAGAC
AflIII 3488 ACGTGT A^C[GA][CT]GT
4018 ACGTGT
Alw44I 2204 GTGCAC G^TGCAC
3254 GTGCAC
ApaLI 2204 GTGCAC G^TGCAC
3254 GTGCAC
BamHI 686 GGATCC G^GATCC
1139 GGATCC
BanI 198 GGTACC G^G[CT][GA]CC
1014 GGCACC
BbsI 1054 GAAGAC GAAGAC(2/6)
1922 GAAGAC
BbvCI 3422 CCTCAGC CCTCAGC(-5/-2)
868 GCTGAGG
BciVI 3524 GGATAC GTATCC(6/5)
751 GTATCC
BlpI 3207 GCTGAGC GC^T[ACGT]AGC
3747 GCTCAGC
Bpu1102I 3207 GCTGAGC GC^T[ACGT]AGC
3747 GCTCAGC
BpuAI 1054 GAAGAC GAAGAC(2/6)
1922 GAAGAC
BsaI 3937 GAGACC GGTCTC(1/5)
2459 GGTCTC
BsrDI 1407 GCAATG GCAATG(2/0)
3728 GCAATG
CelII 3207 GCTGAGC GC^T[ACGT]AGC
3747 GCTCAGC
DraI 2874 TTTAAA TTT^AAA
3557 TTTAAA
DraIII 1046 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2744 CACACTGTG
MslI 1777 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3668 CATCCAAGTG
PflMI 1427 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1976 CCAGAGTCTGG
SmaI 120 CCCGGG CCC^GGG
791 CCCGGG
TaqI 360 TCGA T^CGA
1680 TCGA
TthHB8I 360 TCGA T^CGA
1680 TCGA
Van91I 1427 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1976 CCAGAGTCTGG
XmaCI 120 CCCGGG C^CCGGG
791 CCCGGG
XmaI 120 CCCGGG C^CCGGG
791 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII AvrII
BalI BanII BbvI BcnI BfaI
BlnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SphI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII