##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I ApaLI AscI Asp700I AspI
AviII BalI BbeI BciVI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiWI
BspEI BsrBI BssHII BstAPI BstEII
BstPI CelII CpoI EaeI EagI
EclXI Eco52I EcoO65I FauI FseI
FspI Hin1I HindIII KasI KspI
MluI MluNI MroI MscI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PflFI PinAI PmeI PshAI
Psp1406I RsrII SacII SalI SapI
SbfI SfoI SgrAI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1560 GGTACC G^GTACC
AccII 1385 CGCG CG^CG
Aor51HI 290 AGCGCT AGC^GCT
ApaI 1430 GGGCCC GGGCC^C
Asp718I 1560 GGTACC G^GTACC
AspHI 459 GAGCTC G[AT]GC[AT]^C
AvrII 3328 CCTAGG C^CTAGG
BamHI 581 GGATCC G^GATCC
BbrPI 1078 CACGTG CAC^GTG
BcgI 30 GCAATCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1819 TGATCA T^GATCA
BglI 686 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2055 AGATCT A^GATCT
BlnI 3328 CCTAGG C^CTAGG
BsgI 2237 CTGCAC GTGCAG(16/14)
BsiEI 72 CGATCG CG[GA][CT]^CG
BsiHKAI 459 GAGCTC G[AT]GC[AT]^C
BsmBI 171 CGTCTC CGTCTC(1/5)
BsmI 1231 GAATGC GAATGC(1/-1)
Bsp1407I 3311 TGTACA T^GTACA
BsrDI 921 GCAATG GCAATG(2/0)
BsrFI 653 GCCGGT [GA]^CCGG[CT]
BsrGI 3311 TGTACA T^GTACA
Bst1107I 1579 GTATAC GTA^TAC
BstUI 1385 CGCG CG^CG
BstZ17I 1579 GTATAC GTA^TAC
Cfr10I 653 GCCGGT [GA]^CCGG[CT]
DraIII 2593 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3266 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3241 GAAGAG CTCTTC(1/4)
Eco47III 290 AGCGCT AGC^GCT
EcoNI 1041 CCTCCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 986 GATATC GAT^ATC
EcoT22I 1158 ATGCAT ATGCA^T
FbaI 1819 TGATCA T^GATCA
HaeII 290 AGCGCT [GA]GCGC^[CT]
HgaI 2253 GCGTC GACGC(5/10)
HincII 2246 GTTAAC GT[CT]^[GA]AC
HindII 2246 GTTAAC GT[CT]^[GA]AC
HpaI 2246 GTTAAC GTT^AAC
KpnI 1560 GGTACC GGTAC^C
Ksp632I 3241 GAAGAG CTCTTC(1/4)
MfeI 3451 CAATTG C^AATTG
MunI 3451 CAATTG C^AATTG
MvnI 1385 CGCG CG^CG
NsiI 1158 ATGCAT ATGCA^T
PmaCI 1078 CACGTG CAC^GTG
PmlI 1078 CACGTG CAC^GTG
PvuI 72 CGATCG CGAT^CG
SacI 459 GAGCTC GAGCT^C
ScaI 2929 AGTACT AGT^ACT
SfiI 685 GGCCTAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 734 CCCGGG CCC^GGG
SspBI 3311 TGTACA T^GTACA
XmaCI 734 CCCGGG C^CCGGG
XmaI 734 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 2374 CTTAAG C^TTAAG
2695 CTTAAG
AocI 1509 CCTCAGG CC^T[ACGT]AGG
3250 CCTTAGG
AseI 1488 ATTAAT AT^TAAT
2420 ATTAAT
AsnI 1488 ATTAAT AT^TAAT
2420 ATTAAT
BanI 1560 GGTACC G^G[CT][GA]CC
3133 GGCACC
BbvCI 777 CCTCAGC CCTCAGC(-5/-2)
783 CCTCAGC
BfrI 2374 CTTAAG C^TTAAG
2695 CTTAAG
BsaAI 1078 CACGTG [CT]AC^GT[GA]
2166 CACGTA
BsaBI 569 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2056 GATCTTAATC
BspDI 70 ATCGAT AT^CGAT
413 ATCGAT
BspHI 463 TCATGA T^CATGA
1169 TCATGA
BspLU11I 620 ACATGT A^CATGT
2231 ACATGT
BssSI 1354 CACGAG CACGAG(-5/-1)
1012 CTCGTG
BstBI 2888 TTCGAA TT^CGAA
3463 TTCGAA
BstXI 1396 CCAGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCAGGGTCCTGG
Bsu36I 1509 CCTCAGG CC^T[ACGT]AGG
3250 CCTTAGG
ClaI 70 ATCGAT AT^CGAT
413 ATCGAT
Eco81I 1509 CCTCAGG CC^T[ACGT]AGG
3250 CCTTAGG
EcoRI 2 GAATTC G^AATTC
850 GAATTC
MamI 569 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2056 GATCTTAATC
MslI 941 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1164 CATGGTCATG
NcoI 64 CCATGG C^CATGG
1330 CCATGG
NheI 1692 GCTAGC G^CTAGC
3478 GCTAGC
NspV 2888 TTCGAA TT^CGAA
3463 TTCGAA
PaeR7I 1045 CTCGAG C^TCGAG
1309 CTCGAG
PflMI 930 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
945 CCAGTGGTTGG
PshBI 1488 ATTAAT AT^TAAT
2420 ATTAAT
PstI 552 CTGCAG CTGCA^G
877 CTGCAG
RcaI 463 TCATGA T^CATGA
1169 TCATGA
SexAI 896 ACCTGGT A^CC[AT]GGT
2594 ACCTGGT
SfuI 2888 TTCGAA TT^CGAA
3463 TTCGAA
Van91I 930 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
945 CCAGTGGTTGG
XcmI 1120 CCAGGGGCTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1910 CCAGAAATCCACTGG
XhoI 1045 CTCGAG C^TCGAG
1309 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
AspEI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI Eam1105I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII