################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AcyI AgeI Alw44I ApaLI AscI Asp700I AspI AviII BalI BbeI BciVI BlpI Bpu1102I BsaHI BsaWI BseAI BsiWI BspEI BsrBI BssHII BstAPI BstEII BstPI CelII CpoI EaeI EagI EclXI Eco52I EcoO65I FauI FseI FspI Hin1I HindIII KasI KspI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NotI NruI PacI PflFI PinAI PmeI PshAI Psp1406I RsrII SacII SalI SapI SbfI SfoI SgrAI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 1560 GGTACC G^GTACC AccII 1385 CGCG CG^CG Aor51HI 290 AGCGCT AGC^GCT ApaI 1430 GGGCCC GGGCC^C Asp718I 1560 GGTACC G^GTACC AspHI 459 GAGCTC G[AT]GC[AT]^C AvrII 3328 CCTAGG C^CTAGG BamHI 581 GGATCC G^GATCC BbrPI 1078 CACGTG CAC^GTG BcgI 30 GCAATCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1819 TGATCA T^GATCA BglI 686 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2055 AGATCT A^GATCT BlnI 3328 CCTAGG C^CTAGG BsgI 2237 CTGCAC GTGCAG(16/14) BsiEI 72 CGATCG CG[GA][CT]^CG BsiHKAI 459 GAGCTC G[AT]GC[AT]^C BsmBI 171 CGTCTC CGTCTC(1/5) BsmI 1231 GAATGC GAATGC(1/-1) Bsp1407I 3311 TGTACA T^GTACA BsrDI 921 GCAATG GCAATG(2/0) BsrFI 653 GCCGGT [GA]^CCGG[CT] BsrGI 3311 TGTACA T^GTACA Bst1107I 1579 GTATAC GTA^TAC BstUI 1385 CGCG CG^CG BstZ17I 1579 GTATAC GTA^TAC Cfr10I 653 GCCGGT [GA]^CCGG[CT] DraIII 2593 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3266 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 3241 GAAGAG CTCTTC(1/4) Eco47III 290 AGCGCT AGC^GCT EcoNI 1041 CCTCCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 986 GATATC GAT^ATC EcoT22I 1158 ATGCAT ATGCA^T FbaI 1819 TGATCA T^GATCA HaeII 290 AGCGCT [GA]GCGC^[CT] HgaI 2253 GCGTC GACGC(5/10) HincII 2246 GTTAAC GT[CT]^[GA]AC HindII 2246 GTTAAC GT[CT]^[GA]AC HpaI 2246 GTTAAC GTT^AAC KpnI 1560 GGTACC GGTAC^C Ksp632I 3241 GAAGAG CTCTTC(1/4) MfeI 3451 CAATTG C^AATTG MunI 3451 CAATTG C^AATTG MvnI 1385 CGCG CG^CG NsiI 1158 ATGCAT ATGCA^T PmaCI 1078 CACGTG CAC^GTG PmlI 1078 CACGTG CAC^GTG PvuI 72 CGATCG CGAT^CG SacI 459 GAGCTC GAGCT^C ScaI 2929 AGTACT AGT^ACT SfiI 685 GGCCTAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 734 CCCGGG CCC^GGG SspBI 3311 TGTACA T^GTACA XmaCI 734 CCCGGG C^CCGGG XmaI 734 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflII 2374 CTTAAG C^TTAAG 2695 CTTAAG AocI 1509 CCTCAGG CC^T[ACGT]AGG 3250 CCTTAGG AseI 1488 ATTAAT AT^TAAT 2420 ATTAAT AsnI 1488 ATTAAT AT^TAAT 2420 ATTAAT BanI 1560 GGTACC G^G[CT][GA]CC 3133 GGCACC BbvCI 777 CCTCAGC CCTCAGC(-5/-2) 783 CCTCAGC BfrI 2374 CTTAAG C^TTAAG 2695 CTTAAG BsaAI 1078 CACGTG [CT]AC^GT[GA] 2166 CACGTA BsaBI 569 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2056 GATCTTAATC BspDI 70 ATCGAT AT^CGAT 413 ATCGAT BspHI 463 TCATGA T^CATGA 1169 TCATGA BspLU11I 620 ACATGT A^CATGT 2231 ACATGT BssSI 1354 CACGAG CACGAG(-5/-1) 1012 CTCGTG BstBI 2888 TTCGAA TT^CGAA 3463 TTCGAA BstXI 1396 CCAGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1472 CCAGGGTCCTGG Bsu36I 1509 CCTCAGG CC^T[ACGT]AGG 3250 CCTTAGG ClaI 70 ATCGAT AT^CGAT 413 ATCGAT Eco81I 1509 CCTCAGG CC^T[ACGT]AGG 3250 CCTTAGG EcoRI 2 GAATTC G^AATTC 850 GAATTC MamI 569 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2056 GATCTTAATC MslI 941 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1164 CATGGTCATG NcoI 64 CCATGG C^CATGG 1330 CCATGG NheI 1692 GCTAGC G^CTAGC 3478 GCTAGC NspV 2888 TTCGAA TT^CGAA 3463 TTCGAA PaeR7I 1045 CTCGAG C^TCGAG 1309 CTCGAG PflMI 930 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 945 CCAGTGGTTGG PshBI 1488 ATTAAT AT^TAAT 2420 ATTAAT PstI 552 CTGCAG CTGCA^G 877 CTGCAG RcaI 463 TCATGA T^CATGA 1169 TCATGA SexAI 896 ACCTGGT A^CC[AT]GGT 2594 ACCTGGT SfuI 2888 TTCGAA TT^CGAA 3463 TTCGAA Van91I 930 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 945 CCAGTGGTTGG XcmI 1120 CCAGGGGCTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1910 CCAGAAATCCACTGG XhoI 1045 CTCGAG C^TCGAG 1309 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI ApoI AspEI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI Eam1105I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII