##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI AvrII BbeI BbrPI
BbvCI BcgI BfrI BglI BlnI
BsaBI BsaHI BsiEI BsiWI BsmI
BspDI BsrBI BsrFI BssHII BstAPI
BstBI BstUI Bsu36I Cfr10I ClaI
CpoI EagI EclXI Eco52I Eco81I
EcoRI FauI FseI Hin1I HindIII
KasI KspI MamI MluI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Aor51HI 414 AGCGCT AGC^GCT
ApaI 3104 GGGCCC GGGCC^C
AspI 2851 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 710 TGCGCA TGC^GCA
BamHI 599 GGATCC G^GATCC
BciVI 2550 GGATAC GTATCC(6/5)
BclI 3057 TGATCA T^GATCA
BlpI 1399 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1399 GCTGAGC GC^T[ACGT]AGC
BspMI 1937 ACCTGC ACCTGC(4/8)
BstEII 3372 GGTAACC G^GT[ACGT]ACC
BstPI 3372 GGTAACC G^GT[ACGT]ACC
CelII 1399 GCTGAGC GC^T[ACGT]AGC
DraI 3937 TTTAAA TTT^AAA
DraIII 1535 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3430 GACATCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3206 CCATGG C^C[GA][CT]GG
Eco47III 414 AGCGCT AGC^GCT
EcoO65I 3372 GGTAACC G^GT[ACGT]ACC
EcoRV 4222 GATATC GAT^ATC
EcoT22I 907 ATGCAT ATGCA^T
FbaI 3057 TGATCA T^GATCA
FspI 710 TGCGCA TGC^GCA
HgaI 2676 GCGTC GACGC(5/10)
MfeI 1463 CAATTG C^AATTG
MunI 1463 CAATTG C^AATTG
NcoI 3206 CCATGG C^CATGG
NsiI 907 ATGCAT ATGCA^T
PflFI 2851 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2802 AGGACCT [GA]G^G[AT]CC[CT]
SbfI 1938 CCTGCAGG CCTGCA^GG
SmlI 2617 CTCAAG C^T[CT][GA]AG
SpeI 190 ACTAGT A^CTAGT
Sse8387I 1938 CCTGCAGG CCTGCA^GG
SspI 4343 AATATT AAT^ATT
Tth111I 2851 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1506 GGTACC G^GTACC
3227 GGTACC
AccIII 854 TCCGGA T^CCGGA
2927 TCCGGA
AhdI 423 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
800 GACAGGAGGTC
Asp700I 88 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3043 GAATCTCTTC
Asp718I 1506 GGTACC G^GTACC
3227 GGTACC
AspEI 423 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
800 GACAGGAGGTC
BanI 1506 GGTACC G^G[CT][GA]CC
3227 GGTACC
BsaAI 932 TACGTG [CT]AC^GT[GA]
3520 TACGTG
BseAI 854 TCCGGA T^CCGGA
2927 TCCGGA
BsmBI 3038 GAGACG CGTCTC(1/5)
3485 GAGACG
Bsp1407I 1832 TGTACA T^GTACA
4281 TGTACA
BspEI 854 TCCGGA T^CCGGA
2927 TCCGGA
BspLU11I 1876 ACATGT A^CATGT
3533 ACATGT
BsrGI 1832 TGTACA T^GTACA
4281 TGTACA
BssSI 2624 CACGAG CACGAG(-5/-1)
2738 CACGAG
Bst1107I 1472 GTATAC GTA^TAC
2455 GTATAC
BstXI 2399 CCATCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3515 CCATCTACGTGG
BstZ17I 1472 GTATAC GTA^TAC
2455 GTATAC
Eam1105I 423 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
800 GACAGGAGGTC
EcoNI 795 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1782 CCTCTCAGAGG
HpaI 4167 GTTAAC GTT^AAC
4257 GTTAAC
KpnI 1506 GGTACC GGTAC^C
3227 GGTACC
MroI 854 TCCGGA T^CCGGA
2927 TCCGGA
NheI 1723 GCTAGC G^CTAGC
2307 GCTAGC
PvuII 2171 CAGCTG CAG^CTG
3505 CAGCTG
SacI 1063 GAGCTC GAGCT^C
3142 GAGCTC
SapI 2671 GAAGAGC GCTCTTC(1/4)
3391 GAAGAGC
SmaI 2642 CCCGGG CCC^GGG
3455 CCCGGG
SspBI 1832 TGTACA T^GTACA
4281 TGTACA
XbaI 486 TCTAGA T^CTAGA
2386 TCTAGA
XmaCI 2642 CCCGGG C^CCGGG
3455 CCCGGG
XmaI 2642 CCCGGG C^CCGGG
3455 CCCGGG
XmnI 88 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3043 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PshAI PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII