################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AocI Aor51HI ApaI AscI Asp718I AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BcnI BglI BglII BlnI BsaAI BsaWI BseAI BsgI BsiWI BsoBI Bsp1407I BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HgaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 3231 GACGTC GACGT^C AcyI 3231 GACGTC G[GA]^CG[CT]C AhdI 1580 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4306 GTGCAC G^TGCAC ApaLI 4306 GTGCAC G^TGCAC AspEI 1580 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 3665 GGTGCC G^G[CT][GA]CC BbvCI 1952 GCTGAGG CCTCAGC(-5/-2) BlpI 1235 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1235 GCTGAGC GC^T[ACGT]AGC BsaHI 3231 GACGTC G[GA]^CG[CT]C BseRI 1379 GAGGAG GAGGAG(10/8) BsiEI 1931 CGGTCG CG[GA][CT]^CG BsmBI 33 GAGACG CGTCTC(1/5) BsmFI 2446 GGGAC GGGAC(10/14) BsmI 1750 GAATGC GAATGC(1/-1) BspDI 2297 ATCGAT AT^CGAT BsrBI 3008 GAGCGG CCGCTC(-3/-3) BsrDI 3532 CATTGC GCAATG(2/0) BsrFI 2371 GCCGGT [GA]^CCGG[CT] BstYI 3642 AGATCC [GA]^GATC[CT] CelII 1235 GCTGAGC GC^T[ACGT]AGC Cfr10I 2371 GCCGGT [GA]^CCGG[CT] ClaI 2297 ATCGAT AT^CGAT DraII 2687 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 1545 TGGCCG [CT]^GGCC[GA] Eam1105I 1580 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 2687 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 57 CCAAGG C^C[AT][AT]GG HapII 2372 CCGG C^CGG Hin1I 3231 GACGTC G[GA]^CG[CT]C HpaI 1321 GTTAAC GTT^AAC HpaII 2372 CCGG C^CGG MflI 3642 AGATCC [GA]^GATC[CT] MspI 2372 CCGG C^CGG NheI 4505 GCTAGC G^CTAGC PpuMI 2687 AGGACCC [GA]G^G[AT]CC[CT] ScaI 674 AGTACT AGT^ACT SexAI 2592 ACCAGGT A^CC[AT]GGT StyI 57 CCAAGG C^C[AT][AT]GG XcmI 3717 CCAATAATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 3642 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1856 GTAGAC GT^[AC][GT]AC 2360 GTAGAC AflIII 292 ACATGT A^C[GA][CT]GT 4472 ACATGT BsaBI 3637 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3806 GATTCTGATC BsaI 3503 GAGACC GGTCTC(1/5) 3487 GGTCTC BspLU11I 292 ACATGT A^CATGT 4472 ACATGT BspMI 462 ACCTGC ACCTGC(4/8) 4130 GCAGGT BssSI 2706 CACGAG CACGAG(-5/-1) 4119 CTCGTG BstBI 486 TTCGAA TT^CGAA 3880 TTCGAA DsaI 1209 CCACGG C^C[GA][CT]GG 2485 CCACGG EcoRI 184 GAATTC G^AATTC 592 GAATTC HaeII 418 GGCGCT [GA]GCGC^[CT] 2019 AGCGCC HphI 2374 GGTGA GGTGA(8/7) 3373 GGTGA MamI 3637 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3806 GATTCTGATC MslI 287 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1703 CATGCCAGTG NspV 486 TTCGAA TT^CGAA 3880 TTCGAA PflMI 361 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2485 CCACGGCCTGG PstI 2258 CTGCAG CTGCA^G 4128 CTGCAG SapI 2636 GAAGAGC GCTCTTC(1/4) 2699 GCTCTTC SfuI 486 TTCGAA TT^CGAA 3880 TTCGAA SpeI 769 ACTAGT A^CTAGT 3152 ACTAGT SwaI 2055 ATTTAAAT ATTT^AAAT 2130 ATTTAAAT Van91I 361 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2485 CCACGGCCTGG XbaI 91 TCTAGA T^CTAGA 841 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BanII BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII FbaI Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HindIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MnlI MseI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI