##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AocI Aor51HI ApaI AscI Asp718I
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BcnI BglI BglII BlnI BsaAI
BsaWI BseAI BsgI BsiWI BsoBI
Bsp1407I BspEI BspHI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FseI FspI HgaI KasI
KpnI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3231 GACGTC GACGT^C
AcyI 3231 GACGTC G[GA]^CG[CT]C
AhdI 1580 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4306 GTGCAC G^TGCAC
ApaLI 4306 GTGCAC G^TGCAC
AspEI 1580 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 3665 GGTGCC G^G[CT][GA]CC
BbvCI 1952 GCTGAGG CCTCAGC(-5/-2)
BlpI 1235 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1235 GCTGAGC GC^T[ACGT]AGC
BsaHI 3231 GACGTC G[GA]^CG[CT]C
BseRI 1379 GAGGAG GAGGAG(10/8)
BsiEI 1931 CGGTCG CG[GA][CT]^CG
BsmBI 33 GAGACG CGTCTC(1/5)
BsmFI 2446 GGGAC GGGAC(10/14)
BsmI 1750 GAATGC GAATGC(1/-1)
BspDI 2297 ATCGAT AT^CGAT
BsrBI 3008 GAGCGG CCGCTC(-3/-3)
BsrDI 3532 CATTGC GCAATG(2/0)
BsrFI 2371 GCCGGT [GA]^CCGG[CT]
BstYI 3642 AGATCC [GA]^GATC[CT]
CelII 1235 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2371 GCCGGT [GA]^CCGG[CT]
ClaI 2297 ATCGAT AT^CGAT
DraII 2687 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1545 TGGCCG [CT]^GGCC[GA]
Eam1105I 1580 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2687 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 57 CCAAGG C^C[AT][AT]GG
HapII 2372 CCGG C^CGG
Hin1I 3231 GACGTC G[GA]^CG[CT]C
HpaI 1321 GTTAAC GTT^AAC
HpaII 2372 CCGG C^CGG
MflI 3642 AGATCC [GA]^GATC[CT]
MspI 2372 CCGG C^CGG
NheI 4505 GCTAGC G^CTAGC
PpuMI 2687 AGGACCC [GA]G^G[AT]CC[CT]
ScaI 674 AGTACT AGT^ACT
SexAI 2592 ACCAGGT A^CC[AT]GGT
StyI 57 CCAAGG C^C[AT][AT]GG
XcmI 3717 CCAATAATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3642 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1856 GTAGAC GT^[AC][GT]AC
2360 GTAGAC
AflIII 292 ACATGT A^C[GA][CT]GT
4472 ACATGT
BsaBI 3637 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3806 GATTCTGATC
BsaI 3503 GAGACC GGTCTC(1/5)
3487 GGTCTC
BspLU11I 292 ACATGT A^CATGT
4472 ACATGT
BspMI 462 ACCTGC ACCTGC(4/8)
4130 GCAGGT
BssSI 2706 CACGAG CACGAG(-5/-1)
4119 CTCGTG
BstBI 486 TTCGAA TT^CGAA
3880 TTCGAA
DsaI 1209 CCACGG C^C[GA][CT]GG
2485 CCACGG
EcoRI 184 GAATTC G^AATTC
592 GAATTC
HaeII 418 GGCGCT [GA]GCGC^[CT]
2019 AGCGCC
HphI 2374 GGTGA GGTGA(8/7)
3373 GGTGA
MamI 3637 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3806 GATTCTGATC
MslI 287 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1703 CATGCCAGTG
NspV 486 TTCGAA TT^CGAA
3880 TTCGAA
PflMI 361 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2485 CCACGGCCTGG
PstI 2258 CTGCAG CTGCA^G
4128 CTGCAG
SapI 2636 GAAGAGC GCTCTTC(1/4)
2699 GCTCTTC
SfuI 486 TTCGAA TT^CGAA
3880 TTCGAA
SpeI 769 ACTAGT A^CTAGT
3152 ACTAGT
SwaI 2055 ATTTAAAT ATTT^AAAT
2130 ATTTAAAT
Van91I 361 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2485 CCACGGCCTGG
XbaI 91 TCTAGA T^CTAGA
841 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvI BclI BfaI BfrI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
FbaI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MspA1I MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI