##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbrPI BfrI BsaAI BseAI
BsiWI BsmBI Bsp1407I BspEI BspHI
BsrGI BssHII Bst1107I BstZ17I CpoI
Eam1105I Eco47III EcoRI EcoRV FseI
FspI HpaI MluI MroI NaeI
NdeI NgoMIV NheI NruI PacI
PaeR7I PinAI PmaCI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SexAI SgrAI SnaBI SpeI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 620 GACGTC GACGT^C
Acc65I 2208 GGTACC G^GTACC
AcsI 425 AAATTC [GA]^AATT[CT]
ApoI 425 AAATTC [GA]^AATT[CT]
Asp718I 2208 GGTACC G^GTACC
AspI 307 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3950 CCTAGG C^CTAGG
BamHI 3274 GGATCC G^GATCC
BciVI 2327 GTATCC GTATCC(6/5)
BclI 2712 TGATCA T^GATCA
BglII 2899 AGATCT A^GATCT
BlnI 3950 CCTAGG C^CTAGG
BsaWI 2589 ACCGGA [AT]^CCGG[AT]
BsiEI 10 CGGCCG CG[GA][CT]^CG
BsmI 640 GAATGC GAATGC(1/-1)
BspDI 2594 ATCGAT AT^CGAT
BsrFI 3366 ACCGGC [GA]^CCGG[CT]
BssSI 1957 CACGAG CACGAG(-5/-1)
BstAPI 4068 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3030 TTCGAA TT^CGAA
Cfr10I 3366 ACCGGC [GA]^CCGG[CT]
ClaI 2594 ATCGAT AT^CGAT
DraI 2381 TTTAAA TTT^AAA
EagI 10 CGGCCG C^GGCCG
EclXI 10 CGGCCG C^GGCCG
Eco52I 10 CGGCCG C^GGCCG
EcoT22I 3392 ATGCAT ATGCA^T
FbaI 2712 TGATCA T^GATCA
HindIII 734 AAGCTT A^AGCTT
KpnI 2208 GGTACC GGTAC^C
KspI 13 CCGCGG CCGC^GG
MfeI 4142 CAATTG C^AATTG
MunI 4142 CAATTG C^AATTG
NotI 9 GCGGCCGC GC^GGCCGC
NsiI 3392 ATGCAT ATGCA^T
NspV 3030 TTCGAA TT^CGAA
PflFI 307 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 2380 GTTTAAAC GTTT^AAAC
SacII 13 CCGCGG CCGC^GG
SalI 2084 GTCGAC G^TCGAC
SbfI 2119 CCTGCAGG CCTGCA^GG
ScaI 2523 AGTACT AGT^ACT
SfuI 3030 TTCGAA TT^CGAA
SmaI 1524 CCCGGG CCC^GGG
Sse8387I 2119 CCTGCAGG CCTGCA^GG
Tth111I 307 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1524 CCCGGG C^CCGGG
XmaI 1524 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 3132 ACATGT A^C[GA][CT]GT
3501 ACATGT
Alw44I 4080 GTGCAC G^TGCAC
4331 GTGCAC
ApaI 29 GGGCCC GGGCC^C
4166 GGGCCC
ApaLI 4080 GTGCAC G^TGCAC
4331 GTGCAC
Asp700I 454 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4201 GAAGTTCTTC
BbeI 102 GGCGCC GGCGC^C
1427 GGCGCC
BbvCI 1330 CCTCAGC CCTCAGC(-5/-2)
2047 CCTCAGC
BcgI 1708 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3645 GCATTTCCTTCG
BlpI 1628 GCTGAGC GC^T[ACGT]AGC
2541 GCTCAGC
Bpu1102I 1628 GCTGAGC GC^T[ACGT]AGC
2541 GCTCAGC
BsaBI 985 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2900 GATCTGGATC
BspLU11I 3132 ACATGT A^CATGT
3501 ACATGT
CelII 1628 GCTGAGC GC^T[ACGT]AGC
2541 GCTCAGC
DraIII 4069 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4388 CACACTGTG
DrdI 218 GACTCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2216 GACAATGTGGTC
EcoNI 1054 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3950 CCTAGGCCAGG
KasI 102 GGCGCC G^GCGCC
1427 GGCGCC
MamI 985 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2900 GATCTGGATC
NarI 102 GGCGCC GG^CGCC
1427 GGCGCC
NcoI 1413 CCATGG C^CATGG
2755 CCATGG
PflMI 270 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2404 CCAGTCCTTGG
SapI 1949 GAAGAGC GCTCTTC(1/4)
2471 GAAGAGC
SfiI 751 GGCCAACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
816 GGCCTATCCGGCC
SfoI 102 GGCGCC GGC^GCC
1427 GGCGCC
SphI 465 GCATGC GCATG^C
1887 GCATGC
StuI 127 AGGCCT AGG^CCT
815 AGGCCT
Van91I 270 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2404 CCAGTCCTTGG
XbaI 4187 TCTAGA T^CTAGA
4351 TCTAGA
XmnI 454 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4201 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII