################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AclI AcsI AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI BclI BsaBI BsiWI BsmI BspDI BspLU11I Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraIII DrdI Eam1105I Eco81I EcoRI FbaI FseI HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NruI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflII 3623 CTTAAG C^TTAAG Alw44I 1628 GTGCAC G^TGCAC Aor51HI 21 AGCGCT AGC^GCT ApaLI 1628 GTGCAC G^TGCAC BbrPI 1073 CACGTG CAC^GTG BbvCI 3365 GCTGAGG CCTCAGC(-5/-2) BfrI 3623 CTTAAG C^TTAAG BsiEI 1459 CGGCCG CG[GA][CT]^CG Bsp1407I 2046 TGTACA T^GTACA BspHI 570 TCATGA T^CATGA BsrDI 3457 CATTGC GCAATG(2/0) BsrGI 2046 TGTACA T^GTACA BssHII 395 GCGCGC G^CGCGC DraI 4690 TTTAAA TTT^AAA EagI 1459 CGGCCG C^GGCCG EclXI 1459 CGGCCG C^GGCCG Eco47III 21 AGCGCT AGC^GCT Eco52I 1459 CGGCCG C^GGCCG EcoT22I 4228 ATGCAT ATGCA^T HincII 2926 GTCGAC GT[CT]^[GA]AC HindII 2926 GTCGAC GT[CT]^[GA]AC HindIII 4695 AAGCTT A^AGCTT NaeI 1797 GCCGGC GCC^GGC NgoMIV 1797 GCCGGC G^CCGGC NotI 1458 GCGGCCGC GC^GGCCGC NsiI 4228 ATGCAT ATGCA^T PaeR7I 3401 CTCGAG C^TCGAG PmaCI 1073 CACGTG CAC^GTG PmlI 1073 CACGTG CAC^GTG RcaI 570 TCATGA T^CATGA SalI 2926 GTCGAC G^TCGAC ScaI 1092 AGTACT AGT^ACT SexAI 3908 ACCTGGT A^CC[AT]GGT SspBI 2046 TGTACA T^GTACA XhoI 3401 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 246 GACGTC GACGT^C 1151 GACGTC AccIII 1504 TCCGGA T^CCGGA 4668 TCCGGA AviII 738 TGCGCA TGC^GCA 3687 TGCGCA AvrII 3199 CCTAGG C^CTAGG 3658 CCTAGG BamHI 536 GGATCC G^GATCC 3370 GGATCC BbsI 1196 GAAGAC GAAGAC(2/6) 1292 GTCTTC BciVI 2783 GGATAC GTATCC(6/5) 3195 GTATCC BglII 606 AGATCT A^GATCT 3069 AGATCT BlnI 3199 CCTAGG C^CTAGG 3658 CCTAGG BpuAI 1196 GAAGAC GAAGAC(2/6) 1292 GTCTTC BsaWI 1504 TCCGGA [AT]^CCGG[AT] 4668 TCCGGA BseAI 1504 TCCGGA T^CCGGA 4668 TCCGGA BspEI 1504 TCCGGA T^CCGGA 4668 TCCGGA BsrFI 1797 GCCGGC [GA]^CCGG[CT] 2088 ACCGGC Cfr10I 1797 GCCGGC [GA]^CCGG[CT] 2088 ACCGGC EcoNI 2716 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4102 CCTTTGGCAGG FspI 738 TGCGCA TGC^GCA 3687 TGCGCA MroI 1504 TCCGGA T^CCGGA 4668 TCCGGA NcoI 2580 CCATGG C^CATGG 2913 CCATGG SmaI 478 CCCGGG CCC^GGG 2653 CCCGGG TfiI 157 GAATC G^A[AT]TC 375 GAATC Tsp509I 233 AATT ^AATT 4500 AATT XmaCI 478 CCCGGG C^CCGGG 2653 CCCGGG XmaI 478 CCCGGG C^CCGGG 2653 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII