##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AcsI AflIII AgeI
AhdI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
BclI BsaBI BsiWI BsmI BspDI
BspLU11I Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI Eam1105I
Eco81I EcoRI FbaI FseI HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NruI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 3623 CTTAAG C^TTAAG
Alw44I 1628 GTGCAC G^TGCAC
Aor51HI 21 AGCGCT AGC^GCT
ApaLI 1628 GTGCAC G^TGCAC
BbrPI 1073 CACGTG CAC^GTG
BbvCI 3365 GCTGAGG CCTCAGC(-5/-2)
BfrI 3623 CTTAAG C^TTAAG
BsiEI 1459 CGGCCG CG[GA][CT]^CG
Bsp1407I 2046 TGTACA T^GTACA
BspHI 570 TCATGA T^CATGA
BsrDI 3457 CATTGC GCAATG(2/0)
BsrGI 2046 TGTACA T^GTACA
BssHII 395 GCGCGC G^CGCGC
DraI 4690 TTTAAA TTT^AAA
EagI 1459 CGGCCG C^GGCCG
EclXI 1459 CGGCCG C^GGCCG
Eco47III 21 AGCGCT AGC^GCT
Eco52I 1459 CGGCCG C^GGCCG
EcoT22I 4228 ATGCAT ATGCA^T
HincII 2926 GTCGAC GT[CT]^[GA]AC
HindII 2926 GTCGAC GT[CT]^[GA]AC
HindIII 4695 AAGCTT A^AGCTT
NaeI 1797 GCCGGC GCC^GGC
NgoMIV 1797 GCCGGC G^CCGGC
NotI 1458 GCGGCCGC GC^GGCCGC
NsiI 4228 ATGCAT ATGCA^T
PaeR7I 3401 CTCGAG C^TCGAG
PmaCI 1073 CACGTG CAC^GTG
PmlI 1073 CACGTG CAC^GTG
RcaI 570 TCATGA T^CATGA
SalI 2926 GTCGAC G^TCGAC
ScaI 1092 AGTACT AGT^ACT
SexAI 3908 ACCTGGT A^CC[AT]GGT
SspBI 2046 TGTACA T^GTACA
XhoI 3401 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 246 GACGTC GACGT^C
1151 GACGTC
AccIII 1504 TCCGGA T^CCGGA
4668 TCCGGA
AviII 738 TGCGCA TGC^GCA
3687 TGCGCA
AvrII 3199 CCTAGG C^CTAGG
3658 CCTAGG
BamHI 536 GGATCC G^GATCC
3370 GGATCC
BbsI 1196 GAAGAC GAAGAC(2/6)
1292 GTCTTC
BciVI 2783 GGATAC GTATCC(6/5)
3195 GTATCC
BglII 606 AGATCT A^GATCT
3069 AGATCT
BlnI 3199 CCTAGG C^CTAGG
3658 CCTAGG
BpuAI 1196 GAAGAC GAAGAC(2/6)
1292 GTCTTC
BsaWI 1504 TCCGGA [AT]^CCGG[AT]
4668 TCCGGA
BseAI 1504 TCCGGA T^CCGGA
4668 TCCGGA
BspEI 1504 TCCGGA T^CCGGA
4668 TCCGGA
BsrFI 1797 GCCGGC [GA]^CCGG[CT]
2088 ACCGGC
Cfr10I 1797 GCCGGC [GA]^CCGG[CT]
2088 ACCGGC
EcoNI 2716 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4102 CCTTTGGCAGG
FspI 738 TGCGCA TGC^GCA
3687 TGCGCA
MroI 1504 TCCGGA T^CCGGA
4668 TCCGGA
NcoI 2580 CCATGG C^CATGG
2913 CCATGG
SmaI 478 CCCGGG CCC^GGG
2653 CCCGGG
TfiI 157 GAATC G^A[AT]TC
375 GAATC
Tsp509I 233 AATT ^AATT
4500 AATT
XmaCI 478 CCCGGG C^CCGGG
2653 CCCGGG
XmaI 478 CCCGGG C^CCGGG
2653 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII