##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII BamHI
BbeI BbvCI BciVI BsaBI BsiWI
BsoBI BspDI BsrFI BssHII BssSI
BstBI BstEII BstPI BstXI Bsu36I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI FseI FspI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NspV PaeR7I PinAI PmeI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 441 TCCGGA T^CCGGA
AcyI 115 GACGCC G[GA]^CG[CT]C
AflII 3454 CTTAAG C^TTAAG
AflIII 3607 ACATGT A^C[GA][CT]GT
AlwNI 2095 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 2010 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2917 CCTAGG C^CTAGG
BanI 503 GGCACC G^G[CT][GA]CC
BanII 2543 GAGCTC G[GA]GC[CT]^C
BbrPI 2792 CACGTG CAC^GTG
BcgI 1708 GCATTGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1296 TGATCA T^GATCA
BfrI 3454 CTTAAG C^TTAAG
BglI 4204 GCCAAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2917 CCTAGG C^CTAGG
BlpI 1021 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1021 GCTGAGC GC^T[ACGT]AGC
BsaAI 2792 CACGTG [CT]AC^GT[GA]
BsaHI 115 GACGCC G[GA]^CG[CT]C
BsaI 644 GAGACC GGTCTC(1/5)
BsaWI 441 TCCGGA [AT]^CCGG[AT]
BseAI 441 TCCGGA T^CCGGA
BsiEI 1399 CGACCG CG[GA][CT]^CG
BsmBI 1395 GAGACG CGTCTC(1/5)
BsmI 2103 GAATGC GAATGC(1/-1)
BspEI 441 TCCGGA T^CCGGA
BspLU11I 3607 ACATGT A^CATGT
CelII 1021 GCTGAGC GC^T[ACGT]AGC
DrdI 4266 GACTTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1131 GAAGAG CTCTTC(1/4)
EcoNI 3405 CCTCTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 607 ATGCAT ATGCA^T
FbaI 1296 TGATCA T^GATCA
HaeII 127 GGCGCT [GA]GCGC^[CT]
HgaI 115 GACGC GACGC(5/10)
Hin1I 115 GACGCC G[GA]^CG[CT]C
HindIII 856 AAGCTT A^AGCTT
HpaI 1733 GTTAAC GTT^AAC
Ksp632I 1131 GAAGAG CTCTTC(1/4)
MroI 441 TCCGGA T^CCGGA
NcoI 174 CCATGG C^CATGG
NheI 3280 GCTAGC G^CTAGC
NruI 187 TCGCGA TCG^CGA
NsiI 607 ATGCAT ATGCA^T
PflFI 2010 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2792 CACGTG CAC^GTG
PmlI 2792 CACGTG CAC^GTG
PshAI 145 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 93 CTGCAG CTGCA^G
PvuII 1738 CAGCTG CAG^CTG
SacI 2543 GAGCTC GAGCT^C
SapI 1131 GAAGAGC GCTCTTC(1/4)
StuI 328 AGGCCT AGG^CCT
TaqI 2008 TCGA T^CGA
Tth111I 2010 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2008 TCGA T^CGA
XcmI 1601 CCAGTGCCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 81 CGCG CG^CG
188 CGCG
AclI 380 AACGTT AA^CGTT
1770 AACGTT
AlwI 553 GATCC GGATC(4/5)
4146 GGATC
AseI 1163 ATTAAT AT^TAAT
2853 ATTAAT
AsnI 1163 ATTAAT AT^TAAT
2853 ATTAAT
Asp700I 3570 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3987 GAATAATTTC
BalI 68 TGGCCA TGG^CCA
261 TGGCCA
BcnI 52 CCCGG CC^[GC]GG
2374 CCCGG
BsgI 3634 CTGCAC GTGCAG(16/14)
3725 CTGCAC
Bsp1407I 804 TGTACA T^GTACA
2444 TGTACA
BspHI 1673 TCATGA T^CATGA
3672 TCATGA
BsrBI 18 CCGCTC CCGCTC(-3/-3)
38 CCGCTC
BsrGI 804 TGTACA T^GTACA
2444 TGTACA
BstAPI 600 GCAAAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
986 GCATCCATTGC
BstUI 81 CGCG CG^CG
188 CGCG
DraIII 1823 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2031 CACTTTGTG
DsaI 174 CCATGG C^C[GA][CT]GG
510 CCACGG
EaeI 68 TGGCCA [CT]^GGCC[GA]
261 TGGCCA
EcoRV 3229 GATATC GAT^ATC
3719 GATATC
FauI 17 CCCGC CCCGC(4/6)
48 CCCGC
MluNI 68 TGGCCA TGG^CCA
261 TGGCCA
MscI 68 TGGCCA TGG^CCA
261 TGGCCA
MvnI 81 CGCG CG^CG
188 CGCG
NciI 52 CCCGG CC^[GC]GG
2374 CCCGG
PacI 1160 TTAATTAA TTAAT^TAA
2850 TTAATTAA
PpuMI 1050 AGGTCCT [GA]G^G[AT]CC[CT]
1465 AGGACCT
PshBI 1163 ATTAAT AT^TAAT
2853 ATTAAT
Psp1406I 380 AACGTT AA^CGTT
1770 AACGTT
RcaI 1673 TCATGA T^CATGA
3672 TCATGA
ScaI 2362 AGTACT AGT^ACT
3619 AGTACT
SexAI 2779 ACCAGGT A^CC[AT]GGT
4239 ACCTGGT
SspBI 804 TGTACA T^GTACA
2444 TGTACA
SspI 1406 AATATT AAT^ATT
2709 AATATT
XbaI 232 TCTAGA T^CTAGA
2002 TCTAGA
XmnI 3570 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3987 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AspHI AvaII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI Bst1107I BstF5I BstNI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII