################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AclI AcsI AcyI AgeI Aor51HI ApoI AscI AseI AsnI Asp700I AviII BbeI BbrPI BcgI BclI BsaAI BsaBI BsaHI BsiEI BsiWI BspDI BspHI BsrDI BssHII BssSI BstBI ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoT22I FbaI FseI FspI Hin1I HpaI KasI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 1257 TCCGGA T^CCGGA AhdI 415 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2313 GTGCAC G^TGCAC ApaI 749 GGGCCC GGGCC^C ApaLI 2313 GTGCAC G^TGCAC AspEI 415 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 114 CCCGAG C^[CT]CG[GA]G AvrII 4181 CCTAGG C^CTAGG BamHI 3037 GGATCC G^GATCC BglII 3495 AGATCT A^GATCT BlnI 4181 CCTAGG C^CTAGG BlpI 4219 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4219 GCTGAGC GC^T[ACGT]AGC BseAI 1257 TCCGGA T^CCGGA BsmBI 173 GAGACG CGTCTC(1/5) BsmI 793 GAATGC GAATGC(1/-1) BsoBI 114 CCCGAG C^[CT]CG[GA]G BspEI 1257 TCCGGA T^CCGGA BsrBI 3947 CCGCTC CCGCTC(-3/-3) BsrFI 3159 GCCGGT [GA]^CCGG[CT] Bst1107I 4490 GTATAC GTA^TAC BstZ17I 4490 GTATAC GTA^TAC CelII 4219 GCTGAGC GC^T[ACGT]AGC Cfr10I 3159 GCCGGT [GA]^CCGG[CT] DraI 4547 TTTAAA TTT^AAA DsaI 1753 CCATGG C^C[GA][CT]GG Eam1105I 415 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 3999 GATATC GAT^ATC HaeII 1209 AGCGCC [GA]GCGC^[CT] MroI 1257 TCCGGA T^CCGGA NcoI 1753 CCATGG C^CATGG NdeI 2590 CATATG CA^TATG NheI 1169 GCTAGC G^CTAGC SalI 336 GTCGAC G^TCGAC SbfI 533 CCTGCAGG CCTGCA^GG SfiI 3763 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 533 CCTGCAGG CCTGCA^GG StuI 4071 AGGCCT AGG^CCT XbaI 1822 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 2807 GGTACC G^GTACC 3976 GGTACC AflII 2341 CTTAAG C^TTAAG 4417 CTTAAG Asp718I 2807 GGTACC G^GTACC 3976 GGTACC BbvCI 2209 GCTGAGG CCTCAGC(-5/-2) 3360 GCTGAGG BciVI 1679 GGATAC GTATCC(6/5) 4123 GGATAC BfrI 2341 CTTAAG C^TTAAG 4417 CTTAAG Bsp1407I 858 TGTACA T^GTACA 2152 TGTACA BspMI 688 GCAGGT ACCTGC(4/8) 2163 GCAGGT BsrGI 858 TGTACA T^GTACA 2152 TGTACA BstAPI 23 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2259 GCAGGGTCTGC BstEII 3318 GGTGACC G^GT[ACGT]ACC 3687 GGTGACC BstPI 3318 GGTGACC G^GT[ACGT]ACC 3687 GGTGACC EcoO65I 3318 GGTGACC G^GT[ACGT]ACC 3687 GGTGACC HgaI 175 GACGC GACGC(5/10) 1244 GACGC HincII 336 GTCGAC GT[CT]^[GA]AC 1250 GTCAAC HindII 336 GTCGAC GT[CT]^[GA]AC 1250 GTCAAC HindIII 611 AAGCTT A^AGCTT 3871 AAGCTT KpnI 2807 GGTACC GGTAC^C 3976 GGTACC MaeII 1182 ACGT A^CGT 1632 ACGT SacI 1853 GAGCTC GAGCT^C 4222 GAGCTC SspBI 858 TGTACA T^GTACA 2152 TGTACA SspI 3931 AATATT AAT^ATT 4460 AATATT TaqI 93 TCGA T^CGA 337 TCGA TthHB8I 93 TCGA T^CGA 337 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI AlwI AlwNI AocI AspHI AspI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XhoII