##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AcsI AcyI AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I AviII BbeI BbrPI BcgI
BclI BsaAI BsaBI BsaHI BsiEI
BsiWI BspDI BspHI BsrDI BssHII
BssSI BstBI ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoT22I FbaI FseI FspI
Hin1I HpaI KasI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1257 TCCGGA T^CCGGA
AhdI 415 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2313 GTGCAC G^TGCAC
ApaI 749 GGGCCC GGGCC^C
ApaLI 2313 GTGCAC G^TGCAC
AspEI 415 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 114 CCCGAG C^[CT]CG[GA]G
AvrII 4181 CCTAGG C^CTAGG
BamHI 3037 GGATCC G^GATCC
BglII 3495 AGATCT A^GATCT
BlnI 4181 CCTAGG C^CTAGG
BlpI 4219 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4219 GCTGAGC GC^T[ACGT]AGC
BseAI 1257 TCCGGA T^CCGGA
BsmBI 173 GAGACG CGTCTC(1/5)
BsmI 793 GAATGC GAATGC(1/-1)
BsoBI 114 CCCGAG C^[CT]CG[GA]G
BspEI 1257 TCCGGA T^CCGGA
BsrBI 3947 CCGCTC CCGCTC(-3/-3)
BsrFI 3159 GCCGGT [GA]^CCGG[CT]
Bst1107I 4490 GTATAC GTA^TAC
BstZ17I 4490 GTATAC GTA^TAC
CelII 4219 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3159 GCCGGT [GA]^CCGG[CT]
DraI 4547 TTTAAA TTT^AAA
DsaI 1753 CCATGG C^C[GA][CT]GG
Eam1105I 415 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3999 GATATC GAT^ATC
HaeII 1209 AGCGCC [GA]GCGC^[CT]
MroI 1257 TCCGGA T^CCGGA
NcoI 1753 CCATGG C^CATGG
NdeI 2590 CATATG CA^TATG
NheI 1169 GCTAGC G^CTAGC
SalI 336 GTCGAC G^TCGAC
SbfI 533 CCTGCAGG CCTGCA^GG
SfiI 3763 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 533 CCTGCAGG CCTGCA^GG
StuI 4071 AGGCCT AGG^CCT
XbaI 1822 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2807 GGTACC G^GTACC
3976 GGTACC
AflII 2341 CTTAAG C^TTAAG
4417 CTTAAG
Asp718I 2807 GGTACC G^GTACC
3976 GGTACC
BbvCI 2209 GCTGAGG CCTCAGC(-5/-2)
3360 GCTGAGG
BciVI 1679 GGATAC GTATCC(6/5)
4123 GGATAC
BfrI 2341 CTTAAG C^TTAAG
4417 CTTAAG
Bsp1407I 858 TGTACA T^GTACA
2152 TGTACA
BspMI 688 GCAGGT ACCTGC(4/8)
2163 GCAGGT
BsrGI 858 TGTACA T^GTACA
2152 TGTACA
BstAPI 23 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2259 GCAGGGTCTGC
BstEII 3318 GGTGACC G^GT[ACGT]ACC
3687 GGTGACC
BstPI 3318 GGTGACC G^GT[ACGT]ACC
3687 GGTGACC
EcoO65I 3318 GGTGACC G^GT[ACGT]ACC
3687 GGTGACC
HgaI 175 GACGC GACGC(5/10)
1244 GACGC
HincII 336 GTCGAC GT[CT]^[GA]AC
1250 GTCAAC
HindII 336 GTCGAC GT[CT]^[GA]AC
1250 GTCAAC
HindIII 611 AAGCTT A^AGCTT
3871 AAGCTT
KpnI 2807 GGTACC GGTAC^C
3976 GGTACC
MaeII 1182 ACGT A^CGT
1632 ACGT
SacI 1853 GAGCTC GAGCT^C
4222 GAGCTC
SspBI 858 TGTACA T^GTACA
2152 TGTACA
SspI 3931 AATATT AAT^ATT
4460 AATATT
TaqI 93 TCGA T^CGA
337 TCGA
TthHB8I 93 TCGA T^CGA
337 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AspI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI XhoII