##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BalI BamHI
BglI BlnI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BstAPI BstBI ClaI CpoI EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoRV FseI FspI HgaI KpnI
KspI MluI MluNI MroI MscI
NheI NotI NruI NspV PacI
PinAI PmeI PvuI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SpeI SphI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 3655 AACGTT AA^CGTT
AcyI 1261 GGCGCC G[GA]^CG[CT]C
Asp700I 3146 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1261 GGCGCC G^G[CT][GA]CC
BbeI 1261 GGCGCC GGCGC^C
BbrPI 3221 CACGTG CAC^GTG
BcgI 1639 GCAAGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 367 TGATCA T^GATCA
BglII 679 AGATCT A^GATCT
BsaHI 1261 GGCGCC G[GA]^CG[CT]C
BsaI 4018 GGTCTC GGTCTC(1/5)
BsgI 1631 CTGCAC GTGCAG(16/14)
Bsp1407I 4559 TGTACA T^GTACA
BspHI 2815 TCATGA T^CATGA
BsrFI 8 GCCGGC [GA]^CCGG[CT]
BsrGI 4559 TGTACA T^GTACA
BssHII 2866 GCGCGC G^CGCGC
BssSI 524 CACGAG CACGAG(-5/-1)
BstEII 2050 GGTTACC G^GT[ACGT]ACC
BstPI 2050 GGTTACC G^GT[ACGT]ACC
BstXI 3940 CCAACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
DraIII 1795 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 65 GACCCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2050 GGTTACC G^GT[ACGT]ACC
EcoRI 1166 GAATTC G^AATTC
FbaI 367 TGATCA T^GATCA
Hin1I 1261 GGCGCC G[GA]^CG[CT]C
HindIII 2494 AAGCTT A^AGCTT
KasI 1261 GGCGCC G^GCGCC
NaeI 8 GCCGGC GCC^GGC
NarI 1261 GGCGCC GG^CGCC
NgoMIV 8 GCCGGC G^CCGGC
PaeR7I 3256 CTCGAG C^TCGAG
PflMI 1252 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3221 CACGTG CAC^GTG
PmlI 3221 CACGTG CAC^GTG
PshAI 257 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3655 AACGTT AA^CGTT
RcaI 2815 TCATGA T^CATGA
SalI 74 GTCGAC G^TCGAC
SfoI 1261 GGCGCC GGC^GCC
SspBI 4559 TGTACA T^GTACA
Van91I 1252 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3256 CTCGAG C^TCGAG
XmnI 3146 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 2865 CGCG CG^CG
2867 CGCG
AflII 3205 CTTAAG C^TTAAG
4027 CTTAAG
Aor51HI 481 AGCGCT AGC^GCT
3288 AGCGCT
AseI 1105 ATTAAT AT^TAAT
2670 ATTAAT
AsnI 1105 ATTAAT AT^TAAT
2670 ATTAAT
AspHI 1243 GAGCTC G[AT]GC[AT]^C
4307 GAGCTC
BbvCI 3432 CCTCAGC CCTCAGC(-5/-2)
3825 GCTGAGG
BciVI 584 GGATAC GTATCC(6/5)
3959 GGATAC
BfrI 3205 CTTAAG C^TTAAG
4027 CTTAAG
BsaBI 332 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
362 GATTGTGATC
BsiHKAI 1243 GAGCTC G[AT]GC[AT]^C
4307 GAGCTC
BstUI 2865 CGCG CG^CG
2867 CGCG
DsaI 4893 CCATGG C^C[GA][CT]GG
5002 CCATGG
Eco47III 481 AGCGCT AGC^GCT
3288 AGCGCT
EcoT22I 2621 ATGCAT ATGCA^T
4266 ATGCAT
FauI 68 CCCGC CCCGC(4/6)
4690 GCGGG
HpaI 1614 GTTAAC GTT^AAC
4363 GTTAAC
MamI 332 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
362 GATTGTGATC
MfeI 1462 CAATTG C^AATTG
4124 CAATTG
MunI 1462 CAATTG C^AATTG
4124 CAATTG
MvnI 2865 CGCG CG^CG
2867 CGCG
NcoI 4893 CCATGG C^CATGG
5002 CCATGG
NsiI 2621 ATGCAT ATGCA^T
4266 ATGCAT
PshBI 1105 ATTAAT AT^TAAT
2670 ATTAAT
SacI 1243 GAGCTC GAGCT^C
4307 GAGCTC
SmaI 731 CCCGGG CCC^GGG
4883 CCCGGG
SnaBI 3084 TACGTA TAC^GTA
3679 TACGTA
StuI 1537 AGGCCT AGG^CCT
2310 AGGCCT
XmaCI 731 CCCGGG C^CCGGG
4883 CCCGGG
XmaI 731 CCCGGG C^CCGGG
4883 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI Bst1107I BstF5I
BstNI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoII