################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AviII AvrII BalI BamHI BglI BlnI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BstAPI BstBI ClaI CpoI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoRV FseI FspI HgaI KpnI KspI MluI MluNI MroI MscI NheI NotI NruI NspV PacI PinAI PmeI PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 3655 AACGTT AA^CGTT AcyI 1261 GGCGCC G[GA]^CG[CT]C Asp700I 3146 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 1261 GGCGCC G^G[CT][GA]CC BbeI 1261 GGCGCC GGCGC^C BbrPI 3221 CACGTG CAC^GTG BcgI 1639 GCAAGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 367 TGATCA T^GATCA BglII 679 AGATCT A^GATCT BsaHI 1261 GGCGCC G[GA]^CG[CT]C BsaI 4018 GGTCTC GGTCTC(1/5) BsgI 1631 CTGCAC GTGCAG(16/14) Bsp1407I 4559 TGTACA T^GTACA BspHI 2815 TCATGA T^CATGA BsrFI 8 GCCGGC [GA]^CCGG[CT] BsrGI 4559 TGTACA T^GTACA BssHII 2866 GCGCGC G^CGCGC BssSI 524 CACGAG CACGAG(-5/-1) BstEII 2050 GGTTACC G^GT[ACGT]ACC BstPI 2050 GGTTACC G^GT[ACGT]ACC BstXI 3940 CCAACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 8 GCCGGC [GA]^CCGG[CT] DraIII 1795 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 65 GACCCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2050 GGTTACC G^GT[ACGT]ACC EcoRI 1166 GAATTC G^AATTC FbaI 367 TGATCA T^GATCA Hin1I 1261 GGCGCC G[GA]^CG[CT]C HindIII 2494 AAGCTT A^AGCTT KasI 1261 GGCGCC G^GCGCC NaeI 8 GCCGGC GCC^GGC NarI 1261 GGCGCC GG^CGCC NgoMIV 8 GCCGGC G^CCGGC PaeR7I 3256 CTCGAG C^TCGAG PflMI 1252 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3221 CACGTG CAC^GTG PmlI 3221 CACGTG CAC^GTG PshAI 257 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3655 AACGTT AA^CGTT RcaI 2815 TCATGA T^CATGA SalI 74 GTCGAC G^TCGAC SfoI 1261 GGCGCC GGC^GCC SspBI 4559 TGTACA T^GTACA Van91I 1252 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 3256 CTCGAG C^TCGAG XmnI 3146 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 2865 CGCG CG^CG 2867 CGCG AflII 3205 CTTAAG C^TTAAG 4027 CTTAAG Aor51HI 481 AGCGCT AGC^GCT 3288 AGCGCT AseI 1105 ATTAAT AT^TAAT 2670 ATTAAT AsnI 1105 ATTAAT AT^TAAT 2670 ATTAAT AspHI 1243 GAGCTC G[AT]GC[AT]^C 4307 GAGCTC BbvCI 3432 CCTCAGC CCTCAGC(-5/-2) 3825 GCTGAGG BciVI 584 GGATAC GTATCC(6/5) 3959 GGATAC BfrI 3205 CTTAAG C^TTAAG 4027 CTTAAG BsaBI 332 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 362 GATTGTGATC BsiHKAI 1243 GAGCTC G[AT]GC[AT]^C 4307 GAGCTC BstUI 2865 CGCG CG^CG 2867 CGCG DsaI 4893 CCATGG C^C[GA][CT]GG 5002 CCATGG Eco47III 481 AGCGCT AGC^GCT 3288 AGCGCT EcoT22I 2621 ATGCAT ATGCA^T 4266 ATGCAT FauI 68 CCCGC CCCGC(4/6) 4690 GCGGG HpaI 1614 GTTAAC GTT^AAC 4363 GTTAAC MamI 332 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 362 GATTGTGATC MfeI 1462 CAATTG C^AATTG 4124 CAATTG MunI 1462 CAATTG C^AATTG 4124 CAATTG MvnI 2865 CGCG CG^CG 2867 CGCG NcoI 4893 CCATGG C^CATGG 5002 CCATGG NsiI 2621 ATGCAT ATGCA^T 4266 ATGCAT PshBI 1105 ATTAAT AT^TAAT 2670 ATTAAT SacI 1243 GAGCTC GAGCT^C 4307 GAGCTC SmaI 731 CCCGGG CCC^GGG 4883 CCCGGG SnaBI 3084 TACGTA TAC^GTA 3679 TACGTA StuI 1537 AGGCCT AGG^CCT 2310 AGGCCT XmaCI 731 CCCGGG C^CCGGG 4883 CCCGGG XmaI 731 CCCGGG C^CCGGG 4883 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspI AvaI AvaII BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XbaI XcmI XhoII