##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AflIII AgeI
ApaI AscI Asp718I AspI AviII
AvrII BbeI BbrPI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrFI BssHII
Bst1107I BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco52I EcoNI EcoO65I FseI FspI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1117 GTCTAC GT^[AC][GT]AC
AccII 5276 CGCG CG^CG
AclI 179 AACGTT AA^CGTT
AcyI 3216 GACGCC G[GA]^CG[CT]C
AflII 3376 CTTAAG C^TTAAG
Aor51HI 1485 AGCGCT AGC^GCT
BamHI 478 GGATCC G^GATCC
BbsI 3323 GAAGAC GAAGAC(2/6)
BfrI 3376 CTTAAG C^TTAAG
BlpI 5502 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5502 GCTTAGC GC^T[ACGT]AGC
BpuAI 3323 GAAGAC GAAGAC(2/6)
BsaHI 3216 GACGCC G[GA]^CG[CT]C
BsaI 1925 GGTCTC GGTCTC(1/5)
BsmBI 5370 CGTCTC CGTCTC(1/5)
Bsp1407I 3760 TGTACA T^GTACA
BsrGI 3760 TGTACA T^GTACA
BssSI 2367 CTCGTG CACGAG(-5/-1)
BstAPI 626 GCATTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5276 CGCG CG^CG
BstXI 2818 CCAGGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 5502 GCTTAGC GC^T[ACGT]AGC
DrdI 4674 GACGGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1485 AGCGCT AGC^GCT
EcoRI 3918 GAATTC G^AATTC
Hin1I 3216 GACGCC G[GA]^CG[CT]C
HindIII 5500 AAGCTT A^AGCTT
MfeI 1539 CAATTG C^AATTG
MspA1I 380 CAGCTG C[AC]G^C[GT]G
MunI 1539 CAATTG C^AATTG
MvnI 5276 CGCG CG^CG
PshAI 2210 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 179 AACGTT AA^CGTT
PvuII 380 CAGCTG CAG^CTG
SmaI 4132 CCCGGG CCC^GGG
SspBI 3760 TGTACA T^GTACA
SwaI 2991 ATTTAAAT ATTT^AAAT
XcmI 4545 CCATCTCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4132 CCCGGG C^CCGGG
XmaI 4132 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 2635 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4440 GACAGAGAGTC
Alw44I 3074 GTGCAC G^TGCAC
5487 GTGCAC
AocI 2384 CCTCAGG CC^T[ACGT]AGG
3220 CCTCAGG
ApaLI 3074 GTGCAC G^TGCAC
5487 GTGCAC
AspEI 2635 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4440 GACAGAGAGTC
BalI 847 TGGCCA TGG^CCA
2341 TGGCCA
BanI 2279 GGTGCC G^G[CT][GA]CC
4665 GGTGCC
BbvCI 4707 CCTCAGC CCTCAGC(-5/-2)
1154 GCTGAGG
BclI 1167 TGATCA T^GATCA
4309 TGATCA
BglII 35 AGATCT A^GATCT
2773 AGATCT
BsrBI 532 GAGCGG CCGCTC(-3/-3)
4069 GAGCGG
BstBI 1860 TTCGAA TT^CGAA
1881 TTCGAA
Bsu36I 2384 CCTCAGG CC^T[ACGT]AGG
3220 CCTCAGG
EaeI 847 TGGCCA [CT]^GGCC[GA]
2341 TGGCCA
Eam1105I 2635 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4440 GACAGAGAGTC
Eco81I 2384 CCTCAGG CC^T[ACGT]AGG
3220 CCTCAGG
EcoRV 2675 GATATC GAT^ATC
4979 GATATC
EcoT22I 137 ATGCAT ATGCA^T
1270 ATGCAT
FbaI 1167 TGATCA T^GATCA
4309 TGATCA
MluNI 847 TGGCCA TGG^CCA
2341 TGGCCA
MscI 847 TGGCCA TGG^CCA
2341 TGGCCA
NcoI 1581 CCATGG C^CATGG
4554 CCATGG
NsiI 137 ATGCAT ATGCA^T
1270 ATGCAT
NspV 1860 TTCGAA TT^CGAA
1881 TTCGAA
PpuMI 1197 GGGACCC [GA]G^G[AT]CC[CT]
5259 GGGACCT
SacI 3286 GAGCTC GAGCT^C
3927 GAGCTC
SfuI 1860 TTCGAA TT^CGAA
1881 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanII BbvI
BcgI BciVI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI