################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AflIII AgeI ApaI AscI Asp718I AspI AviII AvrII BbeI BbrPI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrFI BssHII Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraIII EagI EclXI Eco52I EcoNI EcoO65I FseI FspI KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1117 GTCTAC GT^[AC][GT]AC AccII 5276 CGCG CG^CG AclI 179 AACGTT AA^CGTT AcyI 3216 GACGCC G[GA]^CG[CT]C AflII 3376 CTTAAG C^TTAAG Aor51HI 1485 AGCGCT AGC^GCT BamHI 478 GGATCC G^GATCC BbsI 3323 GAAGAC GAAGAC(2/6) BfrI 3376 CTTAAG C^TTAAG BlpI 5502 GCTTAGC GC^T[ACGT]AGC Bpu1102I 5502 GCTTAGC GC^T[ACGT]AGC BpuAI 3323 GAAGAC GAAGAC(2/6) BsaHI 3216 GACGCC G[GA]^CG[CT]C BsaI 1925 GGTCTC GGTCTC(1/5) BsmBI 5370 CGTCTC CGTCTC(1/5) Bsp1407I 3760 TGTACA T^GTACA BsrGI 3760 TGTACA T^GTACA BssSI 2367 CTCGTG CACGAG(-5/-1) BstAPI 626 GCATTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 5276 CGCG CG^CG BstXI 2818 CCAGGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 5502 GCTTAGC GC^T[ACGT]AGC DrdI 4674 GACGGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1485 AGCGCT AGC^GCT EcoRI 3918 GAATTC G^AATTC Hin1I 3216 GACGCC G[GA]^CG[CT]C HindIII 5500 AAGCTT A^AGCTT MfeI 1539 CAATTG C^AATTG MspA1I 380 CAGCTG C[AC]G^C[GT]G MunI 1539 CAATTG C^AATTG MvnI 5276 CGCG CG^CG PshAI 2210 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 179 AACGTT AA^CGTT PvuII 380 CAGCTG CAG^CTG SmaI 4132 CCCGGG CCC^GGG SspBI 3760 TGTACA T^GTACA SwaI 2991 ATTTAAAT ATTT^AAAT XcmI 4545 CCATCTCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 4132 CCCGGG C^CCGGG XmaI 4132 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AhdI 2635 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4440 GACAGAGAGTC Alw44I 3074 GTGCAC G^TGCAC 5487 GTGCAC AocI 2384 CCTCAGG CC^T[ACGT]AGG 3220 CCTCAGG ApaLI 3074 GTGCAC G^TGCAC 5487 GTGCAC AspEI 2635 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4440 GACAGAGAGTC BalI 847 TGGCCA TGG^CCA 2341 TGGCCA BanI 2279 GGTGCC G^G[CT][GA]CC 4665 GGTGCC BbvCI 4707 CCTCAGC CCTCAGC(-5/-2) 1154 GCTGAGG BclI 1167 TGATCA T^GATCA 4309 TGATCA BglII 35 AGATCT A^GATCT 2773 AGATCT BsrBI 532 GAGCGG CCGCTC(-3/-3) 4069 GAGCGG BstBI 1860 TTCGAA TT^CGAA 1881 TTCGAA Bsu36I 2384 CCTCAGG CC^T[ACGT]AGG 3220 CCTCAGG EaeI 847 TGGCCA [CT]^GGCC[GA] 2341 TGGCCA Eam1105I 2635 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4440 GACAGAGAGTC Eco81I 2384 CCTCAGG CC^T[ACGT]AGG 3220 CCTCAGG EcoRV 2675 GATATC GAT^ATC 4979 GATATC EcoT22I 137 ATGCAT ATGCA^T 1270 ATGCAT FbaI 1167 TGATCA T^GATCA 4309 TGATCA MluNI 847 TGGCCA TGG^CCA 2341 TGGCCA MscI 847 TGGCCA TGG^CCA 2341 TGGCCA NcoI 1581 CCATGG C^CATGG 4554 CCATGG NsiI 137 ATGCAT ATGCA^T 1270 ATGCAT NspV 1860 TTCGAA TT^CGAA 1881 TTCGAA PpuMI 1197 GGGACCC [GA]G^G[AT]CC[CT] 5259 GGGACCT SacI 3286 GAGCTC GAGCT^C 3927 GAGCTC SfuI 1860 TTCGAA TT^CGAA 1881 TTCGAA ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanII BbvI BcgI BciVI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI