##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AocI
ApaI AscI AseI AsnI Asp718I
AspI AviII BamHI BbeI BbrPI
BciVI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspMI BsrBI BssHII BstBI
Bsu36I CelII ClaI CpoI DrdI
Eco81I EcoNI EcoRI EcoRV FspI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI Sse8387I SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 5 GGCGTC G[GA]^CG[CT]C
AflII 2603 CTTAAG C^TTAAG
AgeI 3216 ACCGGT A^CCGGT
AhdI 311 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3220 GTGCAC G^TGCAC
Aor51HI 1287 AGCGCT AGC^GCT
ApaLI 3220 GTGCAC G^TGCAC
AspEI 311 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3673 CTCGGG C^[CT]CG[GA]G
AvaII 1275 GGTCC G^G[AT]CC
BanII 1939 GGGCTC G[GA]GC[CT]^C
BbvCI 147 CCTCAGC CCTCAGC(-5/-2)
BfrI 2603 CTTAAG C^TTAAG
BsaHI 5 GGCGTC G[GA]^CG[CT]C
BsaI 1756 GGTCTC GGTCTC(1/5)
BsaWI 3216 ACCGGT [AT]^CCGG[AT]
BsoBI 3673 CTCGGG C^[CT]CG[GA]G
BssSI 787 CACGAG CACGAG(-5/-1)
Bst1107I 2097 GTATAC GTA^TAC
BstEII 1675 GGTAACC G^GT[ACGT]ACC
BstPI 1675 GGTAACC G^GT[ACGT]ACC
BstXI 510 CCAACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2097 GTATAC GTA^TAC
DraII 1598 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 994 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 311 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1287 AGCGCT AGC^GCT
EcoO109I 1598 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1675 GGTAACC G^GT[ACGT]ACC
HaeII 1287 AGCGCT [GA]GCGC^[CT]
Hin1I 5 GGCGTC G[GA]^CG[CT]C
HpaI 2299 GTTAAC GTT^AAC
KspI 161 CCGCGG CCGC^GG
PinAI 3216 ACCGGT A^CCGGT
SacII 161 CCGCGG CCGC^GG
SalI 1296 GTCGAC G^TCGAC
SfcI 2673 CTACAG C^T[GA][CT]AG
SphI 2449 GCATGC GCATG^C
XbaI 1151 TCTAGA T^CTAGA
XcmI 632 CCAAGAGTTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 2461 ACATGT A^C[GA][CT]GT
3476 ACATGT
AlwNI 286 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
3636 CAGCCACTG
AvrII 179 CCTAGG C^CTAGG
968 CCTAGG
BanI 918 GGCACC G^G[CT][GA]CC
3677 GGTGCC
BcgI 689 CGAAAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1103 CGAGGCCAATGC
BclI 3091 TGATCA T^GATCA
3373 TGATCA
BglI 190 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
194 GCCGGGCAGGC
BglII 349 AGATCT A^GATCT
2723 AGATCT
BlnI 179 CCTAGG C^CTAGG
968 CCTAGG
BsaAI 1690 TACGTA [CT]AC^GT[GA]
3322 TACGTA
Bsp1407I 2458 TGTACA T^GTACA
3893 TGTACA
BspLU11I 2461 ACATGT A^CATGT
3476 ACATGT
BsrGI 2458 TGTACA T^GTACA
3893 TGTACA
BstAPI 1173 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3587 GCAAGCATTGC
EcoT22I 3492 ATGCAT ATGCA^T
3986 ATGCAT
FbaI 3091 TGATCA T^GATCA
3373 TGATCA
FseI 28 GGCCGGCC GGCCGG^CC
189 GGCCGGCC
HgaI 73 GACGC GACGC(5/10)
6 GCGTC
HindIII 2606 AAGCTT A^AGCTT
2966 AAGCTT
MslI 893 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2450 CATGCAAGTG
NheI 1406 GCTAGC G^CTAGC
1902 GCTAGC
NsiI 3492 ATGCAT ATGCA^T
3986 ATGCAT
PvuII 1014 CAGCTG CAG^CTG
1401 CAGCTG
SnaBI 1690 TACGTA TAC^GTA
3322 TACGTA
SpeI 1257 ACTAGT A^CTAGT
2136 ACTAGT
SspBI 2458 TGTACA T^GTACA
3893 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
BalI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EaeI
EagI EarI EclXI Eco52I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI