##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BalI        BamHI       BbrPI       
BbvCI       BclI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BspDI       BspEI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HpaI        KpnI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NcoI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SalI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SphI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          539  GTCTAC               GT^[AC][GT]AC
AcsI         2502  AAATTT               [GA]^AATT[CT]
AhdI          996  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2276  GTGCAC               G^TGCAC
AlwNI        1814  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          877  CCTGAGG              CC^T[ACGT]AGG
ApaI          255  GGGCCC               GGGCC^C
ApaLI        2276  GTGCAC               G^TGCAC
ApoI         2502  AAATTT               [GA]^AATT[CT]
Asp700I      2003  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         996  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2585  TGCGCA               TGC^GCA
BcgI         2213  CGAGTTCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1808  GCCCTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1834  AGATCT               A^GATCT
BsiEI         125  CGGCCG               CG[GA][CT]^CG
BsmI         1086  GAATGC               GAATGC(1/-1)
BspMI         711  ACCTGC               ACCTGC(4/8)
BsrBI         128  CCGCTC               CCGCTC(-3/-3)
BsrDI        2582  CATTGC               GCAATG(2/0)
BssHII        228  GCGCGC               G^CGCGC
BssSI          22  CTCGTG               CACGAG(-5/-1)
BstAPI        176  GCAAGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1406  CCAACATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        877  CCTGAGG              CC^T[ACGT]AGG
DraII         432  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII       2646  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           45  GACCGGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          125  CGGCCG               [CT]^GGCC[GA]
EagI          125  CGGCCG               C^GGCCG
Eam1105I      996  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         125  CGGCCG               C^GGCCG
Eco52I        125  CGGCCG               C^GGCCG
Eco81I        877  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      432  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FspI         2585  TGCGCA               TGC^GCA
KspI         1547  CCGCGG               CCGC^GG
NheI          676  GCTAGC               G^CTAGC
SacII        1547  CCGCGG               CCGC^GG
SbfI         1810  CCTGCAGG             CCTGCA^GG
ScaI         3258  AGTACT               AGT^ACT
SpeI         2675  ACTAGT               A^CTAGT
Sse8387I     1810  CCTGCAGG             CCTGCA^GG
SspI         3188  AATATT               AAT^ATT
StuI          179  AGGCCT               AGG^CCT
XbaI          885  TCTAGA               T^CTAGA
XcmI         3186  CCAATATTATTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2003  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflIII       2139  ACATGT               A^C[GA][CT]GT
             3300  ACATGT              
AvaI          411  CCCGGG               C^[CT]CG[GA]G
             1621  CCCGGG              
AvaII         303  GGTCC                G^G[AT]CC
             1003  GGTCC               
BanI          154  GGCGCC               G^G[CT][GA]CC
              215  GGCGCC              
BbeI          154  GGCGCC               GGCGC^C
              215  GGCGCC              
BbsI         1180  GAAGAC               GAAGAC(2/6)
              115  GTCTTC              
BpuAI        1180  GAAGAC               GAAGAC(2/6)
              115  GTCTTC              
BsoBI         411  CCCGGG               C^[CT]CG[GA]G
             1621  CCCGGG              
Bsp1407I     1169  TGTACA               T^GTACA
             1419  TGTACA              
BspHI        2289  TCATGA               T^CATGA
             2390  TCATGA              
BspLU11I     2139  ACATGT               A^CATGT
             3300  ACATGT              
BsrFI         123  GCCGGC               [GA]^CCGG[CT]
             2109  GCCGGC              
BsrGI        1169  TGTACA               T^GTACA
             1419  TGTACA              
Cfr10I        123  GCCGGC               [GA]^CCGG[CT]
             2109  GCCGGC              
DraI         2667  TTTAAA               TTT^AAA
             3234  TTTAAA              
HincII       1756  GTTGAC               GT[CT]^[GA]AC
             1915  GTTGAC              
HindII       1756  GTTGAC               GT[CT]^[GA]AC
             1915  GTTGAC              
HindIII      1240  AAGCTT               A^AGCTT
             1584  AAGCTT              
KasI          154  GGCGCC               G^GCGCC
              215  GGCGCC              
NaeI          123  GCCGGC               GCC^GGC
             2109  GCCGGC              
NarI          154  GGCGCC               GG^CGCC
              215  GGCGCC              
NgoMIV        123  GCCGGC               G^CCGGC
             2109  GCCGGC              
PflMI         599  CCAACAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1122  CCAGCCCGTGG         
RcaI         2289  TCATGA               T^CATGA
             2390  TCATGA              
SfoI          154  GGCGCC               GGC^GCC
              215  GGCGCC              
SmaI          411  CCCGGG               CCC^GGG
             1621  CCCGGG              
SspBI        1169  TGTACA               T^GTACA
             1419  TGTACA              
TaqI          505  TCGA                 T^CGA
             1842  TCGA                
Tsp509I      2503  AATT                 ^AATT
             2971  AATT                
TthHB8I       505  TCGA                 T^CGA
             1842  TCGA                
Van91I        599  CCAACAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1122  CCAGCCCGTGG         
XmaCI         411  CCCGGG               C^CCGGG
             1621  CCCGGG              
XmaI          411  CCCGGG               C^CCGGG
             1621  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflII       
AluI        AlwI        AspHI       BanII       BbvI        
BciVI       BcnI        BfaI        BfrI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EarI        
EcoNI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       XhoII