##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BbrPI
BbvCI BclI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BspDI BspEI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SexAI
SfiI SfuI SgrAI SnaBI SphI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 539 GTCTAC GT^[AC][GT]AC
AcsI 2502 AAATTT [GA]^AATT[CT]
AhdI 996 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2276 GTGCAC G^TGCAC
AlwNI 1814 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 877 CCTGAGG CC^T[ACGT]AGG
ApaI 255 GGGCCC GGGCC^C
ApaLI 2276 GTGCAC G^TGCAC
ApoI 2502 AAATTT [GA]^AATT[CT]
Asp700I 2003 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 996 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2585 TGCGCA TGC^GCA
BcgI 2213 CGAGTTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1808 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1834 AGATCT A^GATCT
BsiEI 125 CGGCCG CG[GA][CT]^CG
BsmI 1086 GAATGC GAATGC(1/-1)
BspMI 711 ACCTGC ACCTGC(4/8)
BsrBI 128 CCGCTC CCGCTC(-3/-3)
BsrDI 2582 CATTGC GCAATG(2/0)
BssHII 228 GCGCGC G^CGCGC
BssSI 22 CTCGTG CACGAG(-5/-1)
BstAPI 176 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1406 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 877 CCTGAGG CC^T[ACGT]AGG
DraII 432 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 2646 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 45 GACCGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 125 CGGCCG [CT]^GGCC[GA]
EagI 125 CGGCCG C^GGCCG
Eam1105I 996 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 125 CGGCCG C^GGCCG
Eco52I 125 CGGCCG C^GGCCG
Eco81I 877 CCTGAGG CC^T[ACGT]AGG
EcoO109I 432 AGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 2585 TGCGCA TGC^GCA
KspI 1547 CCGCGG CCGC^GG
NheI 676 GCTAGC G^CTAGC
SacII 1547 CCGCGG CCGC^GG
SbfI 1810 CCTGCAGG CCTGCA^GG
ScaI 3258 AGTACT AGT^ACT
SpeI 2675 ACTAGT A^CTAGT
Sse8387I 1810 CCTGCAGG CCTGCA^GG
SspI 3188 AATATT AAT^ATT
StuI 179 AGGCCT AGG^CCT
XbaI 885 TCTAGA T^CTAGA
XcmI 3186 CCAATATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2003 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 2139 ACATGT A^C[GA][CT]GT
3300 ACATGT
AvaI 411 CCCGGG C^[CT]CG[GA]G
1621 CCCGGG
AvaII 303 GGTCC G^G[AT]CC
1003 GGTCC
BanI 154 GGCGCC G^G[CT][GA]CC
215 GGCGCC
BbeI 154 GGCGCC GGCGC^C
215 GGCGCC
BbsI 1180 GAAGAC GAAGAC(2/6)
115 GTCTTC
BpuAI 1180 GAAGAC GAAGAC(2/6)
115 GTCTTC
BsoBI 411 CCCGGG C^[CT]CG[GA]G
1621 CCCGGG
Bsp1407I 1169 TGTACA T^GTACA
1419 TGTACA
BspHI 2289 TCATGA T^CATGA
2390 TCATGA
BspLU11I 2139 ACATGT A^CATGT
3300 ACATGT
BsrFI 123 GCCGGC [GA]^CCGG[CT]
2109 GCCGGC
BsrGI 1169 TGTACA T^GTACA
1419 TGTACA
Cfr10I 123 GCCGGC [GA]^CCGG[CT]
2109 GCCGGC
DraI 2667 TTTAAA TTT^AAA
3234 TTTAAA
HincII 1756 GTTGAC GT[CT]^[GA]AC
1915 GTTGAC
HindII 1756 GTTGAC GT[CT]^[GA]AC
1915 GTTGAC
HindIII 1240 AAGCTT A^AGCTT
1584 AAGCTT
KasI 154 GGCGCC G^GCGCC
215 GGCGCC
NaeI 123 GCCGGC GCC^GGC
2109 GCCGGC
NarI 154 GGCGCC GG^CGCC
215 GGCGCC
NgoMIV 123 GCCGGC G^CCGGC
2109 GCCGGC
PflMI 599 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1122 CCAGCCCGTGG
RcaI 2289 TCATGA T^CATGA
2390 TCATGA
SfoI 154 GGCGCC GGC^GCC
215 GGCGCC
SmaI 411 CCCGGG CCC^GGG
1621 CCCGGG
SspBI 1169 TGTACA T^GTACA
1419 TGTACA
TaqI 505 TCGA T^CGA
1842 TCGA
Tsp509I 2503 AATT ^AATT
2971 AATT
TthHB8I 505 TCGA T^CGA
1842 TCGA
Van91I 599 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1122 CCAGCCCGTGG
XmaCI 411 CCCGGG C^CCGGG
1621 CCCGGG
XmaI 411 CCCGGG C^CCGGG
1621 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AluI AlwI AspHI BanII BbvI
BciVI BcnI BfaI BfrI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I TspRI XhoII