################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Aor51HI AscI AseI AsnI Asp718I AspI AvrII BalI BamHI BbrPI BbvCI BclI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SexAI SfiI SfuI SgrAI SnaBI SphI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 539 GTCTAC GT^[AC][GT]AC AcsI 2502 AAATTT [GA]^AATT[CT] AhdI 996 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2276 GTGCAC G^TGCAC AlwNI 1814 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 877 CCTGAGG CC^T[ACGT]AGG ApaI 255 GGGCCC GGGCC^C ApaLI 2276 GTGCAC G^TGCAC ApoI 2502 AAATTT [GA]^AATT[CT] Asp700I 2003 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 996 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2585 TGCGCA TGC^GCA BcgI 2213 CGAGTTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1808 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1834 AGATCT A^GATCT BsiEI 125 CGGCCG CG[GA][CT]^CG BsmI 1086 GAATGC GAATGC(1/-1) BspMI 711 ACCTGC ACCTGC(4/8) BsrBI 128 CCGCTC CCGCTC(-3/-3) BsrDI 2582 CATTGC GCAATG(2/0) BssHII 228 GCGCGC G^CGCGC BssSI 22 CTCGTG CACGAG(-5/-1) BstAPI 176 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1406 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 877 CCTGAGG CC^T[ACGT]AGG DraII 432 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 2646 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 45 GACCGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 125 CGGCCG [CT]^GGCC[GA] EagI 125 CGGCCG C^GGCCG Eam1105I 996 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 125 CGGCCG C^GGCCG Eco52I 125 CGGCCG C^GGCCG Eco81I 877 CCTGAGG CC^T[ACGT]AGG EcoO109I 432 AGGCCCT [GA]G^G[ACGT]CC[CT] FspI 2585 TGCGCA TGC^GCA KspI 1547 CCGCGG CCGC^GG NheI 676 GCTAGC G^CTAGC SacII 1547 CCGCGG CCGC^GG SbfI 1810 CCTGCAGG CCTGCA^GG ScaI 3258 AGTACT AGT^ACT SpeI 2675 ACTAGT A^CTAGT Sse8387I 1810 CCTGCAGG CCTGCA^GG SspI 3188 AATATT AAT^ATT StuI 179 AGGCCT AGG^CCT XbaI 885 TCTAGA T^CTAGA XcmI 3186 CCAATATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2003 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 2139 ACATGT A^C[GA][CT]GT 3300 ACATGT AvaI 411 CCCGGG C^[CT]CG[GA]G 1621 CCCGGG AvaII 303 GGTCC G^G[AT]CC 1003 GGTCC BanI 154 GGCGCC G^G[CT][GA]CC 215 GGCGCC BbeI 154 GGCGCC GGCGC^C 215 GGCGCC BbsI 1180 GAAGAC GAAGAC(2/6) 115 GTCTTC BpuAI 1180 GAAGAC GAAGAC(2/6) 115 GTCTTC BsoBI 411 CCCGGG C^[CT]CG[GA]G 1621 CCCGGG Bsp1407I 1169 TGTACA T^GTACA 1419 TGTACA BspHI 2289 TCATGA T^CATGA 2390 TCATGA BspLU11I 2139 ACATGT A^CATGT 3300 ACATGT BsrFI 123 GCCGGC [GA]^CCGG[CT] 2109 GCCGGC BsrGI 1169 TGTACA T^GTACA 1419 TGTACA Cfr10I 123 GCCGGC [GA]^CCGG[CT] 2109 GCCGGC DraI 2667 TTTAAA TTT^AAA 3234 TTTAAA HincII 1756 GTTGAC GT[CT]^[GA]AC 1915 GTTGAC HindII 1756 GTTGAC GT[CT]^[GA]AC 1915 GTTGAC HindIII 1240 AAGCTT A^AGCTT 1584 AAGCTT KasI 154 GGCGCC G^GCGCC 215 GGCGCC NaeI 123 GCCGGC GCC^GGC 2109 GCCGGC NarI 154 GGCGCC GG^CGCC 215 GGCGCC NgoMIV 123 GCCGGC G^CCGGC 2109 GCCGGC PflMI 599 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1122 CCAGCCCGTGG RcaI 2289 TCATGA T^CATGA 2390 TCATGA SfoI 154 GGCGCC GGC^GCC 215 GGCGCC SmaI 411 CCCGGG CCC^GGG 1621 CCCGGG SspBI 1169 TGTACA T^GTACA 1419 TGTACA TaqI 505 TCGA T^CGA 1842 TCGA Tsp509I 2503 AATT ^AATT 2971 AATT TthHB8I 505 TCGA T^CGA 1842 TCGA Van91I 599 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1122 CCAGCCCGTGG XmaCI 411 CCCGGG C^CCGGG 1621 CCCGGG XmaI 411 CCCGGG C^CCGGG 1621 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcyI AfaI AflII AluI AlwI AspHI BanII BbvI BciVI BcnI BfaI BfrI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI XhoII